LOCUS       sequence-322134-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid encoding chPylRS(IPYE) and pylT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Bryson DI, Fan C, Guo LT, Miller C, Soll D, Liu DR
  TITLE     Continuous directed evolution of aminoacyl-tRNA synthetases.
  JOURNAL   Nat Chem Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474.
  PUBMED    29035361
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474."
COMMENT     Sequence Label: pTECH-chPylRS(IPYE)
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atattctcaa cataaaaaac tttgtgtaat acttgtaacg ctacatggag attaactcaa
       61 tctagagggt aactagtatg gataagaagc cgctggatgt tctgatctct gcgaccggtc
      121 tgtggatgtc ccgtaccggc acgctgcaca agatcaagca ctatgagatt tctcgttcta
      181 aaatctacat cgaaatggcg tgtggtgacc atctggttgt gaacaactct cgttcttgtc
      241 gtcccgcacg tgcattccgt tatcataaat accgtaaaac ctgcaaacgt tgtcgtgttt
      301 ctgacgaaga tatcaacaac ttcctgaccc gttctaccga aggcaaaacc tctgttaaag
      361 ttaaagttgt ttctgagccg aaagtgaaaa aagcgatgcc gaaatctgtt tctcgtgcgc
      421 cgaaaccgct ggaaaatccg gtttctgcga aagcgtctac cgacacctct cgttctgttc
      481 cgtctccggc gaaatctacc ccgaactctc cggttccgac ctctgcaagt gcccccgcac
      541 ttacgaagag ccagactgac aggcttgaag tcctgttaaa cccaaaagat gagatttccc
      601 tgaattccgg caagcctttc agggagcttg agtccgaatt gctctctcgc agaaaaaaag
      661 acctgcagca gatctacgcg gaagaaaggg agaattatct ggggaaactc gagcgtgaaa
      721 ttaccaggtt ctttgtggac aggggttttc tggaaataaa atccccgatc ctgatccctc
      781 ttgagtatat cgaaaggatg ggcattgata atgataccga actttcaaaa cagatcttca
      841 gggttgacaa gaacttctgc ctgagacc
//
