LOCUS       sequence-322138-       11537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium
            tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum 
            and recX..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11537)
  AUTHORS   Yan MY, Li SS, Ding XY, Guo XP, Jin Q, Sun YC
  TITLE     A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient 
            Genome Editing in Mycobacterium tuberculosis.
  JOURNAL   mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 
            10.1128/mBio.02364-19.
  PUBMED    31992616
REFERENCE   2  (bases 1 to 11537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2020-01-28"; volume: "11(1). pii: mBio.02364-19. doi"; pages:
            " 10.1128/mBio.02364-19"
COMMENT     Sequence Label: pNHEJ-recX-sacB
FEATURES             Location/Qualifiers
     source          1..11537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(123..142)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             134..949
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(207..226)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        1237..1682
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(1683..1769,1770..3104)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     rep_origin      3201..3789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3690..3709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8014..8025
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccacctgacg tcgggggggg gggaaagcca cgttgtgtct caaaatctct gatgttacat
       61 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
      121 tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa
      181 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
      241 aggtgcgaca atctatcgct tgtatgggaa gccccatgcg ccagagttgt ttctgaaaca
      301 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
      361 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
      421 actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat atcctgattc
      481 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
      541 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
      601 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
      661 acaagtctgg aaagaaatgc ataatctttt gccattctca ccggattcag tcgtcactca
      721 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
      781 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
      841 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
      901 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
      961 taattggttg taacactggc agagcattac gctgacttga cgggacggcg gctttgttga
     1021 ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac
     1081 cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc
     1141 accaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc ctggtatgag
     1201 tcagcaacac cttcttcacg aggcagacct caccatcaca tatacctgcc gttcactatt
     1261 atttagtgaa atgagatatt atgatatttt ctgaattgtg attaaaaagg caactttatg
     1321 cccatgcaac agaaactata aaaaatacag agaatgaaaa gaaacagata gattttttag
     1381 ttctttaggc ccgtagtctg caaatccttt tatgattttc tatcaaacaa aagaggaaaa
     1441 tagaccagtt gcaatccaaa cgagagtcta atagaatgag gtcgaaaagt aaatcgcgcg
     1501 ggtttgttac tgataaagca ggcaagacct aaaatgtgta aagggcaaag tgtatacttt
     1561 ggcgtcaccc cttacatatt ttaggtcttt ttttattgtg cgtaactaac ttgccatctt
     1621 caaacaggag ggctggaaga agcagaccgc taacacagta cataaaaaag gagacatgaa
     1681 cgatgaacat caaaaagttt gcaaaacaag caacagtatt aacctttact accgcactgc
     1741 tggcaggagg cgcaactcaa gcgtttgcga aagaaacgaa ccaaaagcca tataaggaaa
     1801 catacggcat ttcccatatt acacgccatg atatgctgca aatccctgaa cagcaaaaaa
     1861 atgaaaaata tcaagttcct gaattcgatt cgtccacaat taaaaatatc tcttctgcaa
     1921 aaggcctgga cgtttgggac agctggccat tacaaaacgc tgacggcact gtcgcaaact
     1981 atcacggcta ccacatcgtc tttgcattag ccggagatcc taaaaatgcg gatgacacat
     2041 cgatttacat gttctatcaa aaagtcggcg aaacttctat tgacagctgg aaaaacgctg
     2101 gccgcgtctt taaagacagc gacaaattcg atgcaaatga ttctatccta aaagaccaaa
     2161 cacaagaatg gtcaggttca gccacattta catctgacgg aaaaatccgt ttattctaca
     2221 ctgatttctc cggtaaacat tacggcaaac aaacactgac aactgcacaa gttaacgtat
     2281 cagcatcaga cagctctttg aacatcaacg gtgtagagga ttataaatca atctttgacg
     2341 gtgacggaaa aacgtatcaa aatgtacagc agttcatcga tgaaggcaac tacagctcag
     2401 gcgacaacca tacgctgaga gatcctcact acgtagaaga taaaggccac aaatacttag
     2461 tatttgaagc aaacactgga actgaagatg gctaccaagg cgaagaatct ttatttaaca
     2521 aagcatacta tggcaaaagc acatcattct tccgtcaaga aagtcaaaaa cttctgcaaa
     2581 gcgataaaaa acgcacggct gagttagcaa acggcgctct cggtatgatt gagctaaacg
     2641 atgattacac actgaaaaaa gtgatgaaac cgctgattgc atctaacaca gtaacagatg
     2701 aaattgaacg cgcgaacgtc tttaaaatga acggcaaatg gtacctgttc actgactccc
     2761 gcggatcaaa aatgacgatt gacggcatta cgtctaacga tatttacatg cttggttatg
     2821 tttctaattc tttaactggc ccatacaagc cgctgaacaa aactggcctt gtgttaaaaa
     2881 tggatcttga tcctaacgat gtaaccttta cttactcaca cttcgctgta cctcaagcga
     2941 aaggaaacaa tgtcgtgatt acaagctata tgacaaacag aggattctac gcagacaaac
     3001 aatcaacgtt tgcgccaagc ttcctgctga acatcaaagg caagaaaaca tctgttgtca
     3061 aagacagcat ccttgaacaa ggacaattaa cagttaacaa ataaaaacgc aaaagaaaat
     3121 gccgatnncc ggtttattga ctaccggaag cagtgtgttc catgagcgtc agaccccgta
     3181 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3241 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3301 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3361 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3421 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3481 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3541 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3601 agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3661 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3721 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3781 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3841 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3901 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg agcccaccag
     3961 ctccgtaagt tcgggcgctg tgtggctcgt acccgcgcat tcaggcggca gggggtctaa
     4021 cgggtctaag gcggcgtgta cggccgccac agcggctctc agcggcccgg aaacgtcctc
     4081 gaaacgacgc atgtgttcct cctggttggt acaggtggtt gggggtgctc ggctgtcgct
     4141 ggtgttccac caccagggct cgacgggaga gcgggggagt gtgcagttgt ggggtggccc
     4201 ctcagcgaaa tatctgactt ggagctcgtg tcggaccata caccggtgat taatcgtggt
     4261 ctactaccaa gcgtgagcca cgtcgccgac gaatttgagc agctctggct gccgtactgg
     4321 ccgctggcaa gcgacgatct gctcgagggg atctaccgcc aaagccgcgc gtcggcccta
     4381 ggccgccggt acatcgaggc gaacccaaca gcgctggcaa acctgctggt cgtggacgta
     4441 gaccatccag acgcagcgct ccgagcgctc agcgcccggg ggtcccatcc gctgcccaac
     4501 gcgatcgtgg gcaatcgcgc caacggccac gcacacgcag tgtgggcact caacgcccct
     4561 gttccacgca ccgaatacgc gcggcgtaag ccgctcgcat acatggcggc gtgcgccgaa
     4621 ggccttcggc gcgccgtcga tggcgaccgc agttactcag gcctcatgac caaaaacccc
     4681 ggccacatcg cctgggaaac ggaatggctc cactcagatc tctacacact cagccacatc
     4741 gaggccgagc tcggcgcgaa catgccaccg ccgcgctggc gtcagcagac cacgtacaaa
     4801 gcggctccga cgccgctagg gcggaattgc gcactgttcg attccgtcag gttgtgggcc
     4861 tatcgtcccg ccctcatgcg gatctacctg ccgacccgga acgtggacgg actcggccgc
     4921 gcgatctatg ccgagtgcca cgcgcgaaac gccgaatttc cgtgcaacga cgtgtgtccc
     4981 ggaccgctac cggacagcga ggtccgcgcc atcgccaaca gcatttggcg ttggatcaca
     5041 accaagtcgc gcatttgggc ggacgggatc gtggtctacg aggccacact cagtgcgcgc
     5101 cagtcggcca tctcgcggaa gggcgcagca gcgcgcacgg cggcgagcac agttgcgcgg
     5161 cgcgcaaagt ccgcgtcagc catggaggca ttgctatgag cgacggctac agcgacggct
     5221 acaaccggca gccgactgtc cgcaaaaagc ggcgcgtgac cgccgccgaa ggcgctcgaa
     5281 tcaccggact atccgaacgc cacgtcgtcc ggctcgtggc gcaggaacgc agcgagtggc
     5341 tcgccgagca ggctgcacgc cgcgaacgca tccgcgccta tcacgacgac gagggccact
     5401 cttggccgca aacggccaaa catttcgggc tgcatctgga caccgttaag cgactcggct
     5461 atcgggcgag gaaagagcgt gcggcagaac aggaagcggc tcaaaaggcc cacaacgaag
     5521 ccgacaatcc accgctgttc taacgcaatt ggggagcggg tgtcgcgggg gttccgtggg
     5581 gggttccgtt gcaacgggtc ggacaggtaa aagtcctggt agacgctagt tttctggttt
     5641 gggccatgcc tgtctcgttg cgtgtttcgt tgcgcccgtt ttgaatacca gccagacgag
     5701 acggggttct acgaatcttg gtcgatacca agccatttcc gctgaatatc ggggagctca
     5761 ccgccagaat cggtggttgt ggtgatgtac gtggcgaact ccgttgtagt gcctgtggtg
     5821 gcatccgtgg cgcggccgcg gtacgcagtc gatcgtacgc tagttaacta cgtcgacaga
     5881 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
     5941 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
     6001 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga acccacccgg
     6061 ggtgggcgag tttgtcctgc gtgtgctcgg tcgagtaggc tctgggagta cccgtgtgta
     6121 cgaccagcac ggcatacatc atttcgacgc cgagagattc gccgcccgaa atgagcacga
     6181 tccgcatgct tataacagaa aggaggttaa taatgacggt gtcctgcccg cccccgtcga
     6241 cttctgagcg cgaagagcag gcgcgggcac tgtgcctgcg cctgctcacc gcgcgatccc
     6301 gcacccgcgc cgagttagcc ggccagctgg ccaagcgcgg ctaccccgaa gacatcggca
     6361 accgggtatt ggatcggctg gccgccgttg gcctggtgga tgacaccgac ttcgccgaac
     6421 aatgggttca gtccaggcgg gcgaacgcag caaagagcaa gcgcgcgttg gctgccgagc
     6481 tgcacgccaa gggcgtcgac gacgacgtga tcaccacggt gctcgggggc atcgacgccg
     6541 gtgccgaacg ggggcgggcg gaaaagctgg tacgggccag gctgcggcgg gaggtgctga
     6601 tcgacgacgg caccgacgaa gcgcgggtga gccgcaggct ggtggcgatg ttggcgcgcc
     6661 gtgggtacgg ccagaccttg gcgtgcgagg tggttatcgc cgagctggcc gccgagcggg
     6721 agcgccgacg cgtctaataa cgccgaaaac cccgcttcgg cggggttttg ccgcggtctg
     6781 cgttgtcggg aagatttaaa ataaaaaagg ggacctctag ggtccccaaa agcttggcgt
     6841 gaattcataa cgtacccaag aagcacagtg agtcgcggat cgatggcggg cgcggtatcg
     6901 gcgccgccaa cgtcacctcg tcgagagcca ccacctcact cggtgagcgc aagacgtttt
     6961 caccggcttc gcgtaatccg tcggcgccgc gcttgatcaa atcgagcagg gcgactcccg
     7021 gcgccatggc gtagatcgaa ccgtcggaca acaccccgac acgctcaccg tccgcacttc
     7081 gatacgtcac ccatttcatt cgcgcaccac ctcactcggg tttttgtccg ggcgcaaccc
     7141 acgccagctg gattgccgca atcggtcatc gggggtccac tcgctatagc gcacctcccc
     7201 gaccagcgtc ggcgcgacga acgtaacacc cttggcctcg cgcgccggaa gcgcagcgtc
     7261 gaatggggat tcgtcggtat gcagcggcgc cagcgtcttc ttgaggttgg ccaggtcacg
     7321 ttcggtgaag ccggttccaa cacggccggc gaagtgcaaa ccgctcgggc cgggaatgcc
     7381 catgagcaac gatccaatcc cgctgctgcg cccaccctcc ccaactcgcc aaccgccgat
     7441 gacgacttcc tgggtgttcc agtgtttgtc cttgatccac gccgacgagc gccgcccggg
     7501 ttgatagccg gagtcgcggc gcttggcaat gactccctcc caaccgcgct tgtgcgagta
     7561 ggccagcgcc tcggcgccgt caccgggcaa caactccgga acgatcagat tgcccgcact
     7621 gcccagggtt tccagtagct tgcgccggtc gcggtatctg gcccgcagta gcgagcgacc
     7681 gtccagaaag agcaagtcga acgcccagaa ctcgatgcgg gtggcgcgca cccggttctg
     7741 catctcgttg aagctgggca ctccggagga gtcgagggcc accaattccc cgtcgagcac
     7801 cacgtggtga tcagcgagat cggcggccaa tgaacgtaat tgggggtatt ccgcggtgac
     7861 gtcacgtccg ctgcgggagc gcagtcgcag cgcgccatgg tcggcttcga ccagcagccg
     7921 gtagccgtcc cacttgcctt cgaacgccca ctgacccgac tcgagacctg ccaccgaacc
     7981 gtgggaggtg agcatgggcg cgagggtgtc gaaatcgaag gccgtctggt ccttcatgcg
     8041 atgcgccagc cattggtccc cgttggtctg aatcagcgcg taccgccccg agattcgact
     8101 gccgtgcagg ttgacaatca cctccccctt ttccgaaccg tttctcgagt cgtcgcggaa
     8161 cttctcggtg tcataggtgc cggaatccca gatgatcacc ttgccggcgc cgtactcccc
     8221 tttcgggatg ttgccctcga acgtcgcgta ttccagcgga tgatcttcgg tgtgcacggc
     8281 caggtggttc accgacgtgg tttccggcag gtttttcggc accgcccatg acaccagtac
     8341 cccatcacgc tccagcctga agtcgtaatg cagacggcgc gcgtgatgct cttggatcac
     8401 gaaggtatta ccctgcccga caacgggtct ggctcggggc accggctccg gcgttttcga
     8461 cgcgttgcgc atgctgcgat atttgagcag ccggtcgtcg ccggggaggt ccgcgtccag
     8521 cgccgcaatc agatcgccat cgcgggtgag gcgggcaagc acctcgtcgt aggtcagctg
     8581 gcgcagcccc gggtcgtcga gctcctccca gctgcgtggt gcggcgacgg tcggttgttc
     8641 gcgcccacgc agcgaatacg gcgcgatggt ggtcttcgaa ccgctgtttt ggctccagtc
     8701 caggaacact ttcccggccc gcagactctt ggtcatggtc gcggtgacca acgccggcat
     8761 caccgtttcc agttgctgcg cgacccgttt ggccaacacc gtggcgccct tgctgctcac
     8821 cggctcgtcg agcggggtgt agaggtgcag gcccttgcta ccgctggtga gcgggagggt
     8881 actcaggccg atgtcggcga gcagatcgcg caccgcccgc gccacctgcg cgagttgggc
     8941 catcgtcacg ccctcgcccg ggtccaggtc gaacaccaat cgggtggccg gcccgggcgt
     9001 gaggatttcg cctttcgacg tccactcggc aacgaaccgc cactgcggga cgtgcacctc
     9061 gagggcggcc tgttgcgcga tccaggccag cccggtggcg ctgtcgatga tcggataggt
     9121 cgtcgttccc gaccggtgca ccacgctggc acgcggcagc cagtccggcg ccgacgacgc
     9181 cagctgcttt tcgaaaaagg acgcctgatc gacgccgttg ggccagcgtt tgcgagtcgc
     9241 cgggcgcccg gcgatatgcg ggatcatcac ctcggcgatg ccggtgtagt aaccgaagac
     9301 gtcagacttg gtggttccgg tagccgggta cagcaccttg tccgcgttgg tcagtgtcac
     9361 ctgcggacca ttcgcctgtc caggaggcca ggcctgcgcc gaacccatat ggccaacgta
     9421 acctgtcgcc atggcacgtg ggctggtgat cggtgaagcc ctgatcgaca tcgtcgatgg
     9481 gccggacccg gccgagtatg tcgggggcag ccccctcaac gtcgcggtcg gactggccag
     9541 attgggccgc gacgtcgatc tgctgaccca catcggccgc gacgcccgcg gacgacggat
     9601 cgccgagtac attgaatcct caggtgtaca acttgtttcg ggaagccaga cggctgatcg
     9661 gacgcccacc gcgacggcca ggatcggccc cgacggctcg gccacctacg cgttcgacct
     9721 ggaatggcag atacccgaca caccccccgt ggcgccgccg cttctcgtgc acaccggttc
     9781 catcgccgcc gcgcgggaac caggatgctt ggcggtcgcg gcgctgctcg acgcctatcg
     9841 ggccgcggcc acggtgagct tcgatcccaa tgtgcgtccg tcgctgagcg ccgacccgga
     9901 cctgactcgg gaacgcatcc aacgcctcgt cgagcgcagc gacatcatca aggccagtgc
     9961 cgaagacttg cactggatcg accccaccca gcctccggag cagaccgcgc gggcctggct
    10021 ggcctgcggc ccggcgattg tcgcgctgac gttgggcgat cagggcgcag tggcgttctg
    10081 cgcggccggc ccggcaagtg tgcccgccca gccggttcgc gtggtcgata ccgtcggcgc
    10141 cggagacgcg ttcatggccg gtctgctcga cacgctgtgg gagcagggat tgttaggtgc
    10201 cgaccggcgc accgagttgc gcaagatcgg cgtgtcggcg ctgacctctg cactcgaagt
    10261 cgcggccctg acctcggcgt tgaccgttgc ccgtgccggc gccgatctgc cctatcgggc
    10321 cgacctgcgg cagtcgcgat gaccaccgca cgcccgcaat cgggcccggc gagggtgcac
    10381 gaaatgccgc gtgatgcggc gtgtcgccgt gccgacacgc acgctcgcga gctgaccggg
    10441 gctggggcag gcagcaggac tccggtgccg cggcgcactg ttaaccgacc cgggttctgg
    10501 gcatactgac tccatgcgct ccatctggaa gggttcaatc gctttcgggc tggtgaatgt
    10561 gccggtcaag gtgtacagcg ccactcagga ccacgacatc aagttccatc aggtgcacgc
    10621 caaggacaac ggacgcatcc ggtacaagcg ggtatgcgag gtctgtggcg aggtggtcga
    10681 atactccgac ctggcccgcg ccttcgaatc cgacgatggc cagatggtga tcatcaacga
    10741 cgacgacctc gccacgttgc ccgaagatcg cagccgcgaa atcgaggtgc tggaattcgt
    10801 cccagccgaa gaagtcgatc cgatgatgat tgaccgcagc tacttcctgg agcccgactc
    10861 gaagtcctct aaatcgtatg tgctgctggc caagacgctc gccgagacgg agcggatggc
    10921 gatcgtgcat ttcacgctgc gcagcaagac gcggctggcg gcgttgcggg tcaaggactt
    10981 cggcaagcgc gaggtcatgg tggtgcatac cctgttgtgg ccagacgaga tccgcgaccc
    11041 ggatttcccg gtgctggaca aggaggtgga gatcaaaccc gcggagctca agatggccgg
    11101 ccaagtggtg gagtcgatgg cagacgactt caacccggac cgctaccgcg acacctatca
    11161 ggagcaactg caggagctga tcgacgccaa actcgaaggc ggggaagcat ttaccgccga
    11221 agaacaaccc gcagagctgg acgagaccga agatgtctcc gacctgctgg ccaagctgga
    11281 ggcgagcgtg aaagcgcgtt cgggcgacgg gaagtcagcg gcgaagaaga gcaccgccga
    11341 aaaggcgccg gccaagaagg cccgggccaa acaagctgcc aagtcctgac cagcgcccga
    11401 cttgttcaca tatgctcgtc gtgccgccga gaggagtttt tgcgtgaagt ccaaccgatc
    11461 tggcgtcgtg ctcagcctgc tgctcgccag cacattggcg ttgtcggcat gcggaggcca
    11521 ttccagcaat acctcgt
//
