LOCUS       sequence-322151-       11399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BrdU-incorporating plasmid with selectable marker LEU2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11399)
  AUTHORS   Viggiani CJ, Aparicio OM
  TITLE     New vectors for simplified construction of BrdU-Incorporating 
            strains of Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Oct-Nov;23(14-15):1045-51.
  PUBMED    17083135
REFERENCE   2  (bases 1 to 11399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Oct-Nov"; volume: "23(14-15)"; pages: "1045-51"
COMMENT     Sequence Label: p405-BrdU-Inc
FEATURES             Location/Qualifiers
     source          1..11399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..705
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     2160..2176
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2160..2176
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2210..2226)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2251..2270)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2252..2270)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2280..2297)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2280..2296)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2289..2311)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2437..2892
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2524..2543)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2734..2755
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3193..3600
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3601..4695
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(5189..5208)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5308..5330
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5368..5386)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5454..5558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5559..5627,5628..6419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5777..5796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6590..7178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7079..7098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7332..7349
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7466..7487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7502..7507,7508..7525,7526..7532)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7540..7556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7545..7567
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7564..7580
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7564..7580
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7599..7619
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7601..7619
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    8676..8724
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     8749..8768
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(8766..9896)
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLERK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHVGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWWEDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     promoter        complement(9915..10558)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(9999..10022)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca
       61 tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga
      121 gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt
      181 acactgcctc attgatggtg gtacataacg aactaatact gtagccctag acttgatagc
      241 catcatcata tcgaagtttc actacccttt ttccatttgc catctattga agtaataata
      301 ggcgcatgca acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca
      361 tatccgcaat gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca
      421 gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt
      481 tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc
      541 cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac
      601 ctgcaattat taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc
      661 tttttctgca caatatttca agctatacca agcatacaat caactccaag ctcggaattc
      721 gatatcaagc ttatcgataa gcttgatatc gaattcctgc agcccggggg atctatgaca
      781 accagtcacc agcctcagga cagatacaaa gctgtctggc ttatcttctt catgctgggt
      841 ctgggaacgc tgctcccgtg gaattttttc atgacggcca ctcagtattt cacaaaccgc
      901 ctggacatgt cccagaatgt gtccttggtc actgctgaac tgagcaagga cgcccaggcg
      961 tcagccgccc ctgcagcacc cttgcctgag cggaactctc tcagtgccat cttcaacaat
     1021 gtcatgaccc tatgtgccat gctgcccctg ctgttattca cctacctcaa ctccttcctg
     1081 catcagagga tcccccagtc cgtacggatc ctgggcagcc tggtggccat cctgctggtg
     1141 tttctgatca ctgccatcct ggtgaaggtg cagctggatg ctctgccctt ctttgtcatc
     1201 accatgatca agatcgtgct cattaattca tttggtgcca tcctgcaggg cagcctgttt
     1261 ggtctggctg gccttctgcc tgccagctac acggccccca tcatgagtgg ccagggccta
     1321 gcaggcttct ttgcctccgt ggccatgatc tgcgctattg ccagtggctc ggagctatca
     1381 gaaagtgcct tcggctactt tatcacagcc tgtgctgtta tcattttgac catcatctgt
     1441 tacctgggcc tgccccgcct ggaattctac cgctactacc agcagctcaa gcttgaagga
     1501 cccggggagc aggagaccaa gttggacctc attagcaaag gagaggagcc aagagcaggc
     1561 aaagaggaat ctggagtttc agtctccaac tctcagccca ccaatgaaag ccactctatc
     1621 aaagccatcc tgaaaaatat ctcagtcctg gctttctctg tctgcttcat cttcactatc
     1681 accattggga tgtttccagc cgtgactgtt gaggtcaagt ccagcatcgc aggcagcagc
     1741 acctgggaac gttacttcat tcctgtgtcc tgtttcttga ctttcaatat ctttgactgg
     1801 ttgggccgga gcctcacagc tgtattcatg tggcctggga aggacagccg ctggctgcca
     1861 agcctggtgc tggcccggct ggtgtttgtg ccactgctgc tgctgtgcaa cattaagccc
     1921 cgccgctacc tgactgtggt cttcgagcac gatgcctggt tcatcttctt catggctgcc
     1981 tttgccttct ccaacggcta cctcgccagc ctctgcatgt gcttcgggcc caagaaagtg
     2041 aagccagctg aggcagagac cgcaggagcc atcatggcct tcttcctgtg tctgggtctg
     2101 gcactggggg ctgttttctc cttcctgttc cgggcaattg tgtgactcga ggcggccgct
     2161 ctagaactag tggatccccc gggctgcagg aattcgatat caagcttatc gataccgtcg
     2221 acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     2281 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     2341 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     2401 ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa
     2461 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2521 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2581 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2641 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     2701 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     2761 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     2821 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2881 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     2941 catatcgacc ggtcgaggag aacttctagt atatctacat acctaatatt attgccttat
     3001 taaaaatgga atcccaacaa ttacatcaaa atccacattc tcttcaaaat caattgtcct
     3061 gtacttcctt gttcatgtgt gttcaaaaac gttatattta taggataatt atactctatt
     3121 tctcaacaag taattggttg tttggccgag cggtctaagg cgcctgattc aagaaatatc
     3181 ttgaccgcag ttaactgtgg gaatactcag gtatcgtaag atgcaagagt tcgaatctct
     3241 tagcaaccat tatttttttc ctcaacataa cgagaacaca caggggcgct atcgcacaga
     3301 atcaaattcg atgactggaa attttttgtt aatttcagag gtcgcctgac gcatatacct
     3361 ttttcaactg aaaaattggg agaaaaagga aaggtgagag cgccggaacc ggcttttcat
     3421 atagaataga gaagcgttca tgactaaatg cttgcatcac aatacttgaa gttgacaata
     3481 ttatttaagg acctattgtt ttttccaata ggtggttagc aatcgtctta ctttctaact
     3541 tttcttacct tttacatttc agcaatatat atatatatat ttcaaggata taccattcta
     3601 atgtctgccc ctaagaagat cgtcgttttg ccaggtgacc acgttggtca agaaatcaca
     3661 gccgaagcca ttaaggttct taaagctatt tctgatgttc gttccaatgt caagttcgat
     3721 ttcgaaaatc atttaattgg tggtgctgct atcgatgcta caggtgttcc acttccagat
     3781 gaggcgctgg aagcctccaa gaaggctgat gccgttttgt taggtgctgt gggtggtcct
     3841 aaatggggta ccggtagtgt tagacctgaa caaggtttac taaaaatccg taaagaactt
     3901 caattgtacg ccaacttaag accatgtaac tttgcatccg actctctttt agacttatct
     3961 ccaatcaagc cacaatttgc taaaggtact gacttcgttg ttgtcagaga attagtggga
     4021 ggtatttact ttggtaagag aaaggaagac gatggtgatg gtgtcgcttg ggatagtgaa
     4081 caatacaccg ttccagaagt gcaaagaatc acaagaatgg ccgctttcat ggccctacaa
     4141 catgagccac cattgcctat ttggtccttg gataaagcta atgttttggc ctcttcaaga
     4201 ttatggagaa aaactgtgga ggaaaccatc aagaacgaat tccctacatt gaaggttcaa
     4261 catcaattga ttgattctgc cgccatgatc ctagttaaga acccaaccca cctaaatggt
     4321 attataatca ccagcaacat gtttggtgat atcatctccg atgaagcctc cgttatccca
     4381 ggttccttgg gtttgttgcc atctgcgtcc ttggcctctt tgccagacaa gaacaccgca
     4441 tttggtttgt acgaaccatg ccacggttct gctccagatt tgccaaagaa taaggtcaac
     4501 cctatcgcca ctatcttgtc tgctgcaatg atgttgaaat tgtcattgaa cttgcctgaa
     4561 gaaggtaagg ccattgaaga tgcagttaaa aaggttttgg atgcaggtat cagaactggt
     4621 gatttaggtg gttccaacag taccaccgaa gtcggtgatg ctgtcgccga agaagttaag
     4681 aaaatccttg cttaaaaaga ttctcttttt ttatgatatt tgtacataaa ctttataaat
     4741 gaaattcata atagaaacga cacgaaatta caaaatggaa tatgttcata gggtagacga
     4801 aactatatac gcaatctaca tacatttatc aagaaggaga aaaaggagga tgtaaaggaa
     4861 tacaggtaag caaattgata ctaatggctc aacgtgataa ggaaaaagaa ttgcacttta
     4921 acattaatat tgacaaggag gagggcacca cacaaaaagt taggtgtaac agaaaatcat
     4981 gaaactatga ttcctaattt atatattgga ggattttctc taaaaaaaaa aaaatacaac
     5041 aaataaaaaa cactcaatga cctgaccatt tgatggagtt taagtcaata ccttcttgaa
     5101 ccatttccca taatggtgaa agttccctca agaattttac tctgtcagaa acggccttaa
     5161 cgacgtagtc gatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5221 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5281 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5341 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5401 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5461 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5521 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5581 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5641 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5701 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5761 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5821 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5881 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5941 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6001 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6061 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6121 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6181 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6241 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6301 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6361 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6421 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6481 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6541 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6601 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6661 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6721 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6781 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6841 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6901 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6961 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7021 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7081 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7141 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7201 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7261 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7321 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7381 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7441 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7501 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7561 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7621 aacaaaagct ggagctcggt acccggggat ctagtggatc cgcgaattcc cgcccgtagc
     7681 gcgcgcgcac cagcgcggca tcgcacccga gcaccagctc ccccgtcgtc cagatgccca
     7741 cgggccacgt cgaggccgac ggggagaaat acacgtacct acctggggat ctcaacaggc
     7801 cccgggtggc caaccaggtc gtggacgcgt tgtgcaggtg cgtgatgtcc agctccgtcg
     7861 tcgggtgccg ccgggcccca accggcggtc gggggggcgg tgtatcacgc ggcccgctcg
     7921 ggtggctcgc cgtcgccacg ttgtctcccc gcgggaacgt cagggcctcg gggtcaggga
     7981 cggccgaaaa cgttacccag gcccgggaac gcagcaacac ggaggcggtt ggattgtgca
     8041 agagaccctt aaggggggcg accgcggggg gaggctgggc ggtcggctcg accgtgatgg
     8101 gggcgggcag gctcgcgttc gggggccggc cgagtaggta ggtcttcgag atgtaaagca
     8161 gctggccggg gtcccgcgga aactcggccg tggtgaccaa tacaaaacaa aagcgctcct
     8221 cgtaccagcg aagaaggggc agagatgccg tagtcaggtt tagttcgtcc ggcggcgcca
     8281 gaaatccgcg cggtggtttt tgggggtcgg gggtgtttgg cagccacaga cgcccggtgt
     8341 tcgtgtcgcg ccagtacatg cggtccatgc ccaggccatc caaaaaccat gggtctgtct
     8401 gctcagtcca gtcgtggacc tgaccccacg caacgcccaa aagaataacc cccacgaacc
     8461 ataaaccatt ccccatgggg gaccccgtcc ctaacccacg gggcccgtgg ctatggcagg
     8521 gcttgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg ggggttgggg
     8581 tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg gggtatcgac
     8641 agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa cacccgtgcg
     8701 ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc
     8761 gtgtttcagt tagcctcccc catctcccgg gcaaacgtgc gcgccaggtc gcagatcgtc
     8821 ggtatggagc ctggggtggt gacgtgggtc tggaccatcc cggaggtaag ttgcagcagg
     8881 gcgtcccggc agccggcggg cgattggtcg taatccagga taaagacgtg catgggacgg
     8941 aggcgtttgg ccaagacgtc caaggcccag gcaaacacgt tatacaggtc gccgttgggg
     9001 gccagcaact cgggggcccg aaacagggta aataacgtgt ccccgatatg gggtcgtggg
     9061 cccgcgttgc tctggggctc ggcaccctgg ggcggcacgg ccgtccccga aagctgtccc
     9121 caatcctccc accacgaccc gccgccctgc agataccgca ccgtattggc aagcagcccg
     9181 taaacgcggc gaatcgcggc cagcatagcc aggtcaagcc gctcgccggg gcgctggcgt
     9241 ttggccaggc ggtcgatgtg tctgtcctcc ggaagggccc ccaacacgat gtttgtgccg
     9301 ggcaaggtcg gcgggatgag ggccacgaac gccagcacgg cctggggggt catgctgccc
     9361 ataaggtatc gcgcggccgg gtagcacagg agggcggcga tgggatggcg gtcgaagatg
     9421 agggtgaggg ccgggggcgg ggcatgtgaa ctcccagcct cccccccgac atgaggagcc
     9481 agaacggcgt cggtcacggc ataaggcatg cccattgtta tctgggcgct tgtcattacc
     9541 accgccgcgt ccccggccga tatctcaccc tggtcgaggc ggtgttgtgt ggtgtagatg
     9601 ttcgcgattg tctcggaagc ccccagcacc tgccagtaag tcatcggctc gggtacgtag
     9661 acgatatcgt cgcgcgaacc cagggccacc agcagttgcg tggtggtggt tttccccatc
     9721 ccgtgaggac cgtctatata aacccgcagt agcgtgggca ttttccgctc caggcggact
     9781 tccgtggctt cttgctgccg gcgagggcgc aacgccgtac gtcggttgct atggccgcga
     9841 gaacgcgcag cctggtcgaa cgcagacgcg tgttgatggc aggggtacga agccatggtg
     9901 aattcgcgga tccgtcgaaa ctaagttctt ggtgttttaa aactaaaaaa aagactaact
     9961 ataaaagtag aatttaagaa gtttaagaaa tagatttaca gaattacaat caatacctac
    10021 cgtctttata tacttattag tcaagtaggg gaataatttc agggaactgg tttcaacctt
    10081 ttttttcagc tttttccaaa tcagagagag cagaaggtaa tagaaggtgt aagaaaatga
    10141 gatagataca tgcgtgggtc aattgccttg tgtcatcatt tactccaggc aggttgcatc
    10201 actccattga ggttgtgccc gttttttgcc tgtttgtgcc cctgttctct gtagttgcgc
    10261 taagagaatg gacctatgaa ctgatggttg gtgaagaaaa caatattttg gtgctgggat
    10321 tctttttttt tctggatgcc agcttaaaaa gcgggctcca ttatatttag tggatgccag
    10381 gaataaactg ttcacccaga cacctacgat gttatatatt ctgtgtaacc cgccccctat
    10441 tttgggcatg tacgggttac agcagaatta aaaggctaat tttttgacta aataaagtta
    10501 ggaaaatcac tactattaat tatttacgta ttctttgaaa tggcagtatt gataatgata
    10561 aactcgacct gcagccaagc ttatcgatga taagctgtca aacatgagaa ttcgatatca
    10621 agcttatcga taccgtcgac tctagaggat ccgggatcga agaaatgatg gtaaatgaaa
    10681 taggaaatca aggagcatga aggcaaaaga caaatataag ggtcgaacga aaaataaagt
    10741 gaaaagtgtt gatatgatgt atttggcttt gcggcgccga aaaaacgagt ttacgcaatt
    10801 gcacaatcat gctgactctg tggcggaccc gcgctcttgc cggcccggcg ataacgctgg
    10861 gcgtgaggct gtgcccggcg gagttttttg cgcctgcatt ttccaaggtt taccctgcgc
    10921 taaggggcga gattggagaa gcaataagaa tgccggttgg ggttgcgatg atgacgacca
    10981 cgacaactgg tgtcattatt taagttgccg aaagaacctg agtgcatttg caacatgagt
    11041 atactagaag aatgagccaa gacttgcgag acgcgagttt gccggtggtg cgaacaatag
    11101 agcgaccatg accttgaagg tgagacgcgc ataaccgcta gagtactttg aagaggaaac
    11161 agcaataggg ttgctaccag tataaataga caggtacata caacactgga aatggttgtc
    11221 tgtttgagta cgctttcaat tcatttgggt gtgcacttta ttatgttaca atatggaagg
    11281 gaactttaca cttctcctat gcacatatat taattaaagt ccaatgctag tagagaaggg
    11341 gggtaacacc cctccgcgct cttttccgat ttttttctaa accgtggaat atttcggat
//
