LOCUS       sequence-322168-        7130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV for glutamate reporter fused to TARP gamma-8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Hao Y, Toulme E, Konig B, Rosenmund C, Plested AJR
  TITLE     Targeted sensors for glutamatergic neurotransmission.
  JOURNAL   eLife. 2023 Jan 9;12:e84029. doi: 10.7554/eLife.84029.
  PUBMED    36622100
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2023-01-9"; volume: "12:e84029. doi"; pages: " 
            10.7554/eLife.84029"
COMMENT     Sequence Label: pAAV-syn-SnFR-gamma8-minWPRE
FEATURES             Location/Qualifiers
     source          1..7130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        173..620
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     sig_peptide     712..771
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     1740..1761
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1867..1888)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2128..2147)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2358..2376
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(3998..4018)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   4393..4533
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4393..4522
                     /label=AAV2 ITR
     rep_origin      4608..5063
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4695..4714)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4905..4926
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5080..5099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5199..5221
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5259..5277)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5345..5449
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5450..5518,5519..6310)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5668..5687)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6481..7069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6970..6989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtagagg gccctgcgta tgagtgcaag
      181 tgggttttag gaccaggatg aggcggggtg ggggtgccta cctgacgacc gaccccgacc
      241 cactggacaa gcacccaacc cccattcccc aaattgcgca tcccctatca gagaggggga
      301 ggggaaacag gatgcggcga ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc
      361 cttcgccccc gcctggcggc gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt
      421 cactcgccgg tcccccgcaa actccccttc ccggccacct tggtcgcgtc cgcgccgccg
      481 ccggcccagc cggaccgcac cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc
      541 tgcgctgcgg cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg
      601 tgtcgtgcct gagagcgcag tcgctagcgt ttaaacttaa gcttggtacc gaactcggat
      661 ccactagtaa cggccgccag tgtgctggaa ttcggcttgg ggatatccac catggagaca
      721 gacacactcc tgctatgggt actgctgctc tgggttccag gttccactgg tgacagatct
      781 gccgcaggca gcacgctgga caaaatcgcc aaaaacggtg tgattgtcgt cggtcaccgt
      841 gaatcttcag tgcctttctc ttattacgac aatcagcaaa aagtggtggg ttactcgcag
      901 gattactcca acgccattgt tgaagcagtg aaaaagaaac tcaacaaacc ggacttgcag
      961 gtaaaactga ttccgattac ctcacaaaac cgtattccac tgctgcaaaa cggcactttc
     1021 gattttgaat gtggttctac caccaacaac gtcgaacgcc aaaaacaggc ggctttctct
     1081 gacactattt tcgtggtcgg tacgcgcctg ttgaccaaaa agggtggcga tatcaaagat
     1141 tttgccaacc tgaaagacaa agccgtagtc gtcacttccg gcactacctc tgaagttttg
     1201 ctcaacaaac tgaatgaaga gcaaaaaatg aatatgcgca tcatcagcgc caaagatcac
     1261 ggtgactctt tccgcaccct ggaaagcggt cgtgccgttg cctttatgat ggatgacgtg
     1321 ctgctggccg gtgaacgtgc gaaagcgaag aaaccagaca actgggaaat cgtcggcaag
     1381 ccgcagtctc aggaggccta cggttgtatg ttgcgtaaag atgatccgca gttcaaaaag
     1441 ctgatggatg acaccatcgc tcaggtgcag acctccggtg aagcggaaaa atggtttgat
     1501 aagtggttca aaaatccaat tctggtgagc cacaacgtct atatcatggc cgacaagcag
     1561 aggaacggca tcaaggcgaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
     1621 ctcgcctacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1681 aaccactacc tgagcaccca gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac
     1741 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1801 aagggcggta ccggagggag catggtgagc aagggcgagg agctgttcac cggggtggtg
     1861 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1921 ggtgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1981 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2041 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2101 atccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2161 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2221 gacggcaaca tcctggggca taagctggag tacaacttta acaaccccct gaacatgaat
     2281 ttcgaactgt cagacgaaat gaaagcactg ttcaaagaac cgaatgacaa ggcactgaaa
     2341 ggtgggcgcg cccgggcaga tgtgtataag agacaggaaa agaaaaaagc aggactgttt
     2401 gaacaaatca cgaagactca tgggacgatc attggtgtca catcagggat cgtcctggtc
     2461 cttctcatta tttctatttt agtacaagtg aaacagcccc ggaaaaaggt catggcttgc
     2521 aaaactgcat ttaataaaac tgggttccag gaagtatttg atcctcctca ttatgaacta
     2581 ttttcactaa gagagaaaga gatttctgca gacgccgggt ccgagtcatt gaaacgctgg
     2641 aatgaagaga gggggttgtg gtgcgaaaag ggcgttcagg tactactgac caccataggc
     2701 gcctttgcag cttttggcct catgaccatc gccatcagca ctgactactg gctctacaca
     2761 agagctctca tctgcaacac caccaacctc acagcaggtg atgatggacc accccatcgt
     2821 gggggcagtg gctcctcaga gaagaaggac cctgggggcc tcacacattc aggcctctgg
     2881 cggatatgct gcctggaagg gttgaaaaga ggtgtctgcg tgaagatcaa ccacttcccg
     2941 gaggacacgg actacgacca cgacagcgcg gagtacctgc tccgagtagt ccgggcctcc
     3001 agcatctttc ctatcctgag cgccatcctg ctgctgctcg ggggcgtgtg cgtagctgcc
     3061 tctcgcgtct acaaatccaa aaggaacatc atcctgggcg cagggatcct gttcgtggca
     3121 gcaggcctga gcaacatcat cggggtgatt gtgtacatat cggccaacgc cggcgagcct
     3181 ggccccaaga gggacgagga gaagaaaaac cactactcgt acggctggtc cttctacttc
     3241 ggcgggctgt ccttcatcct ggctgaggtg atcggagtac tggccgtcaa catctacatc
     3301 gagcgcagcc gcgaggcaca ctgccaatca cgctcggacc tgctcaaggc cggcggcggc
     3361 gcgggcggca gtggcgggag cggcccctcg gccatcctcc gtctgcccag ttaccgcttc
     3421 cgctaccgcc gccgctcccg ctccagctcc cgaggctcca gcgaggcgtc gccatcccgg
     3481 gatgcgtctc ccggcggccc cgggggcccg ggcttcgcct ccacggacat ctccatgtac
     3541 acgctcagcc gcgacccgtc caagggcagc gtggctgcgg ggctggcgag cgccggtggc
     3601 ggcggcagcg gtgccggcgt gggtgcctac ggcggggcgg ccggggcggc ggggggcggc
     3661 ggggcgggct cggagcggga ccgcgggagc tcggcgggtt ttctcacgct gcacaacgcc
     3721 ttccccaagg aagcggcgtc cggcgtcacg gtcacagtca ccggaccgcc cgctgcaccc
     3781 gcgcccgcgc ccgcgccgcc cgctcctgca gcgcccgcgc ccgggaccct gtccaaagag
     3841 gccgcggcgt ccaacaccaa cacgctcaac aggaaaacca cgcccgtgta ggaattctgc
     3901 agatatccag cacagtggcg gccgctcgag tctagactcg agataatcaa cctctggatt
     3961 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4021 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4081 cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg
     4141 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg
     4201 aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat
     4261 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     4321 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag cacgtgcgga
     4381 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     4441 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     4501 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     4561 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     4621 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4681 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4741 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4801 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     4861 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4921 aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc
     4981 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     5041 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     5101 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5161 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5221 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5281 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5341 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5401 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5461 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5521 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5581 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5641 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5701 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5761 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5821 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5881 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5941 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6001 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6061 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6121 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6181 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6241 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6301 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6361 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6421 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6481 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6541 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6601 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     6661 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6721 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6781 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6841 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6901 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6961 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7021 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7081 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
