LOCUS       sequence-322249-        1217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression and localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, 
            Mathur P, Schauer K, Goud B, Albanese V, Gautier R, Subra M, Kovacs 
            D, Barelli H, Antonny B
  TITLE     A comprehensive library of fluorescent constructs of SARS-CoV-2 
            proteins and their initial characterisation in different cell types.
  JOURNAL   Biol Cell. 2021 Jul;113(7):311-328. doi: 10.1111/boc.202000158. Epub
            2021 May 10.
  PUBMED    33666950
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/boc.202000158"; journalName: "Biol Cell"; date: "2021-07"; 
            volume: "113"; issue: "7"; pages: "311-328"
COMMENT     Sequence Label: Nsp10-EGFP
FEATURES             Location/Qualifiers
     source          1..1217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    431..483
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      491..500
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(543..564)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(804..823)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1151..1172
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagccacc atggctggta atgcaactga ggttcccgcg aatagtacgg ttctgagctt
       61 ttgcgccttt gctgttgacg cagctaaggc atataaggac tacttggcct ccgggggcca
      121 gccaatcaca aactgcgtca agatgttgtg tacgcacacg ggcacaggtc aggcaatcac
      181 tgtcacacct gaagctaaca tggatcagga atcttttggt ggtgccagtt gttgtctgta
      241 ctgtcgatgt catatagatc atcccaaccc gaaagggttt tgtgatctga aaggaaaata
      301 cgtccaaatc ccgaccacgt gcgcgaacga cccggttggg tttacgttga aaaataccgt
      361 atgtactgtc tgtggtatgt ggaagggcta tggctgttca tgcgatcaat tgcgagagcc
      421 gatgttgcag ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga
      481 tccaccggtc gccaccatgg tgagcaaggg cgaggaggct gttcaccggg gtggtgccca
      541 tcctggtcga gctggacggc gacgtaracg gccacaagtt cagcgtgtcc ggcgagggcg
      601 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      661 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
      721 accycgacca catgaagcag cacgacttct tcaagtccgc catgcscgaa ggctacgtcc
      781 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      841 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      901 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      961 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     1021 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1081 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     1141 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     1201 acgagctgta caagtaa
//
