LOCUS       sequence-322250-        3081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression and localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, 
            Mathur P, Schauer K, Goud B, Albanese V, Gautier R, Subra M, Kovacs 
            D, Barelli H, Antonny B
  TITLE     A comprehensive library of fluorescent constructs of SARS-CoV-2 
            proteins and their initial characterisation in different cell types.
  JOURNAL   Biol Cell. 2021 Jul;113(7):311-328. doi: 10.1111/boc.202000158. Epub
            2021 May 10.
  PUBMED    33666950
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/boc.202000158"; journalName: "Biol Cell"; date: "2021-07"; 
            volume: "113"; issue: "7"; pages: "311-328"
COMMENT     Sequence Label: Nsp12-EGFP
FEATURES             Location/Qualifiers
     source          1..3081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2810..2862
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      2870..2879
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2921..2942)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gctagccacc atgtcagcag acgcacaaag ttttctgaac cgggtatgcg gagtcagtgc
       61 ggcgaggctt acgccctgtg gtacgggaac atcaacggac gttgtttacc gcgccttcga
      121 catatacaat gataaagtcg ccggcttcgc aaagttcctt aaaacgaatt gctgtaggtt
      181 ccaagaaaag gacgaggatg ataatttgat agactcctac ttcgtggtca aacgacacac
      241 gttttcaaat tatcagcacg aggaaacgat atataatctt cttaaagact gccctgcagt
      301 ggcaaaacac gacttcttta agttccgcat tgacggggat atggtccccc atatctcccg
      361 acagagattg acgaaatata caatggcgga tctggtgtac gccctgagac acttcgatga
      421 gggcaattgt gatacactca aggagattct cgtgacctac aattgttgcg atgatgacta
      481 tttcaataag aaagactggt acgacttcgt cgaaaaccct gatatattga gggtgtacgc
      541 aaacctgggt gaacgagtaa gacaggcact tctgaaaacc gtccaattct gcgacgctat
      601 gagaaacgcg ggcattgttg gtgtgcttac gctggataac caagacctca atggtaattg
      661 gtacgatttt ggggacttca tccaaaccac tcctggctca ggcgttcccg tggtagacag
      721 ctactattct ttgttgatgc caattcttac actcacaagg gccctgaccg ctgagtcaca
      781 cgtcgatacg gacttgacca agccctacat caagtgggat cttctgaagt acgactttac
      841 cgaggaacga ttgaagcttt ttgatcgcta ctttaaatac tgggatcaga cgtaccaccc
      901 aaactgcgtg aattgcctgg atgaccgatg tatactccat tgcgcgaact ttaacgtttt
      961 gttctctaca gtatttcctc cgacgagctt cggaccgctt gtgagaaaga tattcgtcga
     1021 cggcgtccca tttgttgtca gcactggtta ccactttcgc gagttggggg tggtccacaa
     1081 tcaggatgtc aatttgcatt cctccaggct ttcttttaaa gagttgcttg tttatgccgc
     1141 tgaccccgcg atgcatgcag cctctgggaa tcttttgctg gacaaaagga cgacgtgctt
     1201 tagcgttgct gcccttacaa ataacgtagc atttcaaacc gtaaaacccg gtaattttaa
     1261 taaagatttt tatgattttg cagtctctaa gggttttttc aaggagggga gtagtgtaga
     1321 gcttaaacat ttttttttcg ctcaggatgg taacgctgcg atcagcgact acgactacta
     1381 tcgatacaat ctcccgacga tgtgtgatat caggcagttg ttgttcgttg tagaagtggt
     1441 tgataagtat tttgactgtt atgacggcgg ctgtatcaat gccaaccagg tcattgtgaa
     1501 taacctcgat aaatctgccg ggttcccctt taacaagtgg gggaaggcgc gactgtacta
     1561 cgacagcatg tcttacgaag atcaggacgc actctttgca tacacaaaga ggaatgtgat
     1621 accgactata actcaaatga accttaagta tgcaatcagc gcgaaaaatc gggctaggac
     1681 tgttgccgga gttagcatat gtagtaccat gacaaaccga cagttccacc agaagttgct
     1741 gaaatcaatt gctgcgaccc ggggtgcgac tgtagtaatc gggacatcaa agttctacgg
     1801 cgggtggcat aacatgctca agacggtcta ctcagacgtt gagaatccgc atcttatggg
     1861 ttgggattat ccaaagtgtg acagggcgat gcccaacatg ttgcggataa tggcctcctt
     1921 ggtacttgca cggaaacata caacctgctg tagtttgtca caccgatttt accgactggc
     1981 aaacgaatgt gctcaggttc tcagtgaaat ggtaatgtgt ggcggctccc tctacgttaa
     2041 gcccggtggc acaagctctg gagacgcaac tactgcctat gcaaacagtg tattcaacat
     2101 ctgccaggct gtcacggcca atgttaacgc attgctttcc actgacggaa ataagattgc
     2161 agacaaatac gtgagaaatt tgcaacatag actctacgaa tgcctctatc gcaatcgcga
     2221 tgttgatacg gacttcgtta atgagttcta tgcttacttg cgaaagcact tctccatgat
     2281 gattctgtca gacgacgctg tagtatgctt taattcaacg tatgcctccc agggtctggt
     2341 cgcgtccata aaaaatttta aaagcgtcct gtattaccaa aataacgttt tcatgagcga
     2401 agcgaaatgt tggaccgaga ctgatcttac taagggccct catgagttct gctcacaaca
     2461 cactatgttg gtcaagcagg gcgacgatta cgtgtatctt ccttatcccg atcctagtag
     2521 gattcttgga gcaggttgct tcgtggatga tatcgtcaaa acggacggca ccctcatgat
     2581 agagaggttc gtctctttgg ctatagacgc gtatccgctt actaaacatc ctaaccaaga
     2641 atacgcggat gtattccatc tttatctgca gtatatcaga aagttgcatg atgagctgac
     2701 cggtcacatg ttggacatgt atagcgtgat gcttaccaat gacaatactt cacggtactg
     2761 ggaacctgaa ttttacgagg cgatgtatac gcctcacacc gtcctgcagc tcgagctcaa
     2821 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt
     2881 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2941 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3001 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3061 gaccaccctg acctacggcg t
//
