LOCUS       sequence-322251-        3147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression and localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, 
            Mathur P, Schauer K, Goud B, Albanese V, Gautier R, Subra M, Kovacs 
            D, Barelli H, Antonny B
  TITLE     A comprehensive library of fluorescent constructs of SARS-CoV-2 
            proteins and their initial characterisation in different cell types.
  JOURNAL   Biol Cell. 2021 Jul;113(7):311-328. doi: 10.1111/boc.202000158. Epub
            2021 May 10.
  PUBMED    33666950
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/boc.202000158"; journalName: "Biol Cell"; date: "2021-07"; 
            volume: "113"; issue: "7"; pages: "311-328"
COMMENT     Sequence Label: EGFP-Nsp12
FEATURES             Location/Qualifiers
     source          1..3147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2994..3115
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3031..3050)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3085..3104
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaatt
       61 ctatgtcagc agacgcacaa agttttctga accgggtatg cggagtcagt gcggcgaggc
      121 ttacgccctg tggtacggga acatcaacgg acgttgttta ccgcgccttc gacatataca
      181 atgataaagt cgccggcttc gcaaagttcc ttaaaacgaa ttgctgtagg ttccaagaaa
      241 aggacgagga tgataatttg atagactcct acttcgtggt caaacgacac acgttttcaa
      301 attatcagca cgaggaaacg atatataatc ttcttaaaga ctgccctgca gtggcaaaac
      361 acgacttctt taagttccgc attgacgggg atatggtccc ccatatctcc cgacagagat
      421 tgacgaaata tacaatggcg gatctggtgt acgccctgag acacttcgat gagggcaatt
      481 gtgatacact caaggagatt ctcgtgacct acaattgttg cgatgatgac tatttcaata
      541 agaaagactg gtacgacttc gtcgaaaacc ctgatatatt gagggtgtac gcaaacctgg
      601 gtgaacgagt aagacaggca cttctgaaaa ccgtccaatt ctgcgacgct atgagaaacg
      661 cgggcattgt tggtgtgctt acgctggata accaagacct caatggtaat tggtacgatt
      721 ttggggactt catccaaacc actcctggct caggcgttcc cgtggtagac agctactatt
      781 ctttgttgat gccaattctt acactcacaa gggccctgac cgctgagtca cacgtcgata
      841 cggacttgac caagccctac atcaagtggg atcttctgaa gtacgacttt accgaggaac
      901 gattgaagct ttttgatcgc tactttaaat actgggatca gacgtaccac ccaaactgcg
      961 tgaattgcct ggatgaccga tgtatactcc attgcgcgaa ctttaacgtt ttgttctcta
     1021 cagtatttcc tccgacgagc ttcggaccgc ttgtgagaaa gatattcgtc gacggcgtcc
     1081 catttgttgt cagcactggt taccactttc gcgagttggg ggtggtccac aatcaggatg
     1141 tcaatttgca ttcctccagg ctttctttta aagagttgct tgtttatgcc gctgaccccg
     1201 cgatgcatgc agcctctggg aatcttttgc tggacaaaag gacgacgtgc tttagcgttg
     1261 ctgcccttac aaataacgta gcatttcaaa ccgtaaaacc cggtaatttt aataaagatt
     1321 tttatgattt tgcagtctct aagggttttt tcaaggaggg gagtagtgta gagcttaaac
     1381 attttttttt cgctcaggat ggtaacgctg cgatcagcga ctacgactac tatcgataca
     1441 atctcccgac gatgtgtgat atcaggcagt tgttgttcgt tgtagaagtg gttgataagt
     1501 attttgactg ttatgacggc ggctgtatca atgccaacca ggtcattgtg aataacctcg
     1561 ataaatctgc cgggttcccc tttaacaagt gggggaaggc gcgactgtac tacgacagca
     1621 tgtcttacga agatcaggac gcactctttg catacacaaa gaggaatgtg ataccgacta
     1681 taactcaaat gaaccttaag tatgcaatca gcgcgaaaaa tcgggctagg actgttgccg
     1741 gagttagcat atgtagtacc atgacaaacc gacagttcca ccagaagttg ctgaaatcaa
     1801 ttgctgcgac ccggggtgcg actgtagtaa tcgggacatc aaagttctac ggcgggtggc
     1861 ataacatgct caagacggtc tactcagacg ttgagaatcc gcatcttatg ggttgggatt
     1921 atccaaagtg tgacagggcg atgcccaaca tgttgcggat aatggcctcc ttggtacttg
     1981 cacggaaaca tacaacctgc tgtagtttgt cacaccgatt ttaccgactg gcaaacgaat
     2041 gtgctcaggt tctcagtgaa atggtaatgt gtggcggctc cctctacgtt aagcccggtg
     2101 gcacaagctc tggagacgca actactgcct atgcaaacag tgtattcaac atctgccagg
     2161 ctgtcacggc caatgttaac gcattgcttt ccactgacgg aaataagatt gcagacaaat
     2221 acgtgagaaa tttgcaacat agactctacg aatgcctcta tcgcaatcgc gatgttgata
     2281 cggacttcgt taatgagttc tatgcttact tgcgaaagca cttctccatg atgattctgt
     2341 cagacgacgc tgtagtatgc tttaattcaa cgtatgcctc ccagggtctg gtcgcgtcca
     2401 taaaaaattt taaaagcgtc ctgtattacc aaaataacgt tttcatgagc gaagcgaaat
     2461 gttggaccga gactgatctt actaagggcc ctcatgagtt ctgctcacaa cacactatgt
     2521 tggtcaagca gggcgacgat tacgtgtatc ttccttatcc cgatcctagt aggattcttg
     2581 gagcaggttg cttcgtggat gatatcgtca aaacggacgg caccctcatg atagagaggt
     2641 tcgtctcttt ggctatagac gcgtatccgc ttactaaaca tcctaaccaa gaatacgcgg
     2701 atgtattcca tctttatctg cagtatatca gaaagttgca tgatgagctg accggtcaca
     2761 tgttggacat gtatagcgtg atgcttacca atgacaatac ttcacggtac tgggaacctg
     2821 aattttacga ggcgatgtat acgcctcaca ccgtcctgca gtaaggatcc accggatcta
     2881 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     2941 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3001 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3061 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     3121 taaattgtaa gcgttaatat tttgtta
//
