LOCUS       sequence-322258-         741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression and localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, 
            Mathur P, Schauer K, Goud B, Albanese V, Gautier R, Subra M, Kovacs 
            D, Barelli H, Antonny B
  TITLE     A comprehensive library of fluorescent constructs of SARS-CoV-2 
            proteins and their initial characterisation in different cell types.
  JOURNAL   Biol Cell. 2021 Jul;113(7):311-328. doi: 10.1111/boc.202000158. Epub
            2021 May 10.
  PUBMED    33666950
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/boc.202000158"; journalName: "Biol Cell"; date: "2021-07"; 
            volume: "113"; issue: "7"; pages: "311-328"
COMMENT     Sequence Label: EGFP-COV E
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    310..431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(347..366)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     401..420
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(575..596)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtgctcttgt ttctggcatt cgtcgtattt ctcctcgtca cactggcaat tctgactgca
       61 ttgaggcttt gcgcctactg ttgtaacatt gtcaatgtat ctctcgtgaa accctcattc
      121 tacgtttaca gcagggtgaa gaatctcaat tctagcaggg tgccggatct cctcgtttaa
      181 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
      241 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
      301 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
      361 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
      421 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
      481 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
      541 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
      601 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
      661 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
      721 gaaccctaaa gggagccccc g
//
