LOCUS       sequence-322261-        1594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression and localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, 
            Mathur P, Schauer K, Goud B, Albanese V, Gautier R, Subra M, Kovacs 
            D, Barelli H, Antonny B
  TITLE     A comprehensive library of fluorescent constructs of SARS-CoV-2 
            proteins and their initial characterisation in different cell types.
  JOURNAL   Biol Cell. 2021 Jul;113(7):311-328. doi: 10.1111/boc.202000158. Epub
            2021 May 10.
  PUBMED    33666950
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/boc.202000158"; journalName: "Biol Cell"; date: "2021-07"; 
            volume: "113"; issue: "7"; pages: "311-328"
COMMENT     Sequence Label: Nsp4 deltaC-EGFP
FEATURES             Location/Qualifiers
     source          1..1594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     misc_feature    1446..1498
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1506..1515
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1557..1578)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
       61 cagatccgct agccaccatg aagattgtaa acaattggct taaacaactt attaaagtaa
      121 ccctggtatt cttgtttgtc gccgcaatct tttacttgat aacaccggtc cacgtgatgt
      181 ctaaacacac agactttagt agtgagataa tcggatataa agctatcgat ggcggcgtta
      241 cccgcgacat cgcttcaacc gacacatgtt tcgctaataa gcacgccgat tttgacactt
      301 ggtttagtca aagaggagga agctacacaa acgacaaggc ttgtccgctt atcgcggctg
      361 taataacaag ggaagttggg tttgtcgtgc ctggtttgcc aggtactata ctcagaacca
      421 caaacggtga ctttctgcac ttcttgccaa gggtttttag cgcggtaggc aatatctgct
      481 atacgccaag caagctgatt gaatacaccg actttgcgac gagcgcatgt gtcctcgctg
      541 cagagtgtac gatatttaag gatgcgagcg gaaagcccgt accttactgc tatgatacta
      601 atgtccttga gggatcagtt gcgtatgaga gtcttaggcc ggatacacga tatgtgctca
      661 tggatggttc cataatccag ttcccaaata cctacctcga agggtccgtt cgggtcgtca
      721 ccacatttga tagcgaatat tgcagacacg gaacatgcga acggagtgaa gcaggggtct
      781 gcgtctccac aagtgggcgg tgggtcctca ataacgatta ttaccggagc ctgcccggcg
      841 tgttttgcgg ggtcgatgcg gttaacttgt tgacgaatat gtttacgcct ctcatacaac
      901 ccattggcgc actggatata tctgcaagta tagtcgctgg aggcattgtg gcaatcgtgg
      961 ttacgtgttt ggcgtattat tttatgaggt tccgccgggc tttcggggaa tattcccacg
     1021 tagtcgcctt caacacactc ttgtttctca tgtctttcac tgtactgtgt ttgactcctg
     1081 tctactcttt cttgccgggt gtatactcag ttatttactt gtacctcacg ttttatttga
     1141 caaacgatgt ttcatttctc gcgcatatcc aatggatggt gatgtttacg ccattggtgc
     1201 ctttctggat cacgatcgct tatatcattt gcatcagcac caaacacttc tattggttct
     1261 ttagtaacta cttgaagcgc agggttgttt ttaacggtgt cagcttctca acgtttgaag
     1321 aggcagcact gtgtactttc cttctgaata aggaaatgta tctcaagctg cggtccgatg
     1381 tcctgcttcc gcttacccag tataatcgct atctcgccct gtataataag tacaagtact
     1441 tctcactcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac
     1501 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1561 tcgagctgga cggcgacgta aacggccaca agtt
//
