LOCUS       sequence-322263-        1602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression and localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, 
            Mathur P, Schauer K, Goud B, Albanese V, Gautier R, Subra M, Kovacs 
            D, Barelli H, Antonny B
  TITLE     A comprehensive library of fluorescent constructs of SARS-CoV-2 
            proteins and their initial characterisation in different cell types.
  JOURNAL   Biol Cell. 2021 Jul;113(7):311-328. doi: 10.1111/boc.202000158. Epub
            2021 May 10.
  PUBMED    33666950
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/boc.202000158"; journalName: "Biol Cell"; date: "2021-07"; 
            volume: "113"; issue: "7"; pages: "311-328"
COMMENT     Sequence Label: Nsp13 deltaN2-EGFP
FEATURES             Location/Qualifiers
     source          1..1602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1433..1485
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1493..1502
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1544..1565)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        join(1602,1..44)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
       61 agatccgcta gccaccatga ttgcgacggt cagagaggtt ctttccgacc gagaattgca
      121 cctctcatgg gaggtcggta agccgcgccc gcctctgaat aggaactatg tctttacagg
      181 ctatcgagtt acaaaaaaca gtaaggtgca gataggggaa tatacttttg agaaaggtga
      241 ctatggggac gccgtagtat acagggggac aactacctac aaactgaatg tgggcgatta
      301 tttcgtgttg actagccata ctgtcatgcc cctctctgcc ccgaccctcg ttccacagga
      361 acattatgtt cgcattactg ggctgtaccc cacgctcaac atatccgatg aatttagctc
      421 caacgtggca aactaccaga aggtgggaat gcaaaagtat agcacgttgc aaggcccccc
      481 tggcacaggt aaatcccact tcgccattgg cttggcgttg tactacccga gtgcccggat
      541 cgtctatact gcatgctctc atgctgcggt agacgcattg tgtgagaaag ccctgaagta
      601 tctgcccatt gacaagtgca gtcggataat cccagctcgg gcgcgagttg aatgcttcga
      661 taagtttaag gtaaatagta cacttgagca gtatgtattt tgtaccgtca acgctctccc
      721 cgaaaccact gcggacatag tggtatttga tgagatatct atggctacca attatgacct
      781 ctcagtagtc aacgcacgct tgcgagcaaa acactatgta tacatcggag acccagcgca
      841 attgcctgct cctaggactc tcctcaccaa agggactctt gagcctgaat atttcaactc
      901 cgtctgcagg ctgatgaaaa ccatcggacc cgatatgttt ctcgggacat gtcgaagatg
      961 tcctgccgaa attgtagata ctgttagcgc cttggtgtac gataataagc tgaaggccca
     1021 taaagacaag agcgcccagt gtttcaagat gttctataag ggagtaatca cacatgacgt
     1081 tagttctgcc atcaaccgac cgcagatagg cgtggtccgc gagttcctga cccgaaatcc
     1141 agcgtggagg aaggccgtat ttatatcacc ttacaatagc caaaatgccg tggcgtcaaa
     1201 gattttgggg ctcccgactc agacggtgga tagctctcaa gggtcagaat acgattacgt
     1261 tatattcaca cagacgacag agacggcaca cagttgcaac gtcaacagat ttaatgtcgc
     1321 tataacccgc gccaaggttg ggattctttg tataatgtct gacagagacc tctacgacaa
     1381 gctgcagttc acgtcacttg agattcctcg caggaacgtt gcgacgcttc aactcgagct
     1441 caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat
     1501 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1561 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc ga
//
