LOCUS       sequence-322264-         738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression and localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, 
            Mathur P, Schauer K, Goud B, Albanese V, Gautier R, Subra M, Kovacs 
            D, Barelli H, Antonny B
  TITLE     A comprehensive library of fluorescent constructs of SARS-CoV-2 
            proteins and their initial characterisation in different cell types.
  JOURNAL   Biol Cell. 2021 Jul;113(7):311-328. doi: 10.1111/boc.202000158. Epub
            2021 May 10.
  PUBMED    33666950
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/boc.202000158"; journalName: "Biol Cell"; date: "2021-07"; 
            volume: "113"; issue: "7"; pages: "311-328"
COMMENT     Sequence Label: EGFP-Orf9b L52D
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     111..132
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    634..715
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(671..690)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
       61 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      121 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga
      181 ctcagatctc gagctcaagc ttcgaattct atggacccca aaatttctga aatgcacccg
      241 gcgcttcgct tggtggaccc ccaaatccaa ctcgctgtca cgcggatgga aaacgcagta
      301 ggacgcgacc aaaataatgt cggcccaaaa gtgtatccga ttatcctccg ccttggcagt
      361 cccgacagcc tgaatatggc cagaaagact cttaacagcc tggaggacaa agcgtttcag
      421 ctgaccccca ttgcggttca gatgactaag ctggcgacaa ctgaagagtt gcccgacgaa
      481 tttgtggtag taaccgtaaa gtaaggatcc accggatcta gataactgat cataatcagc
      541 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacaycd ccccctgaac
      601 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
      661 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
      721 agttgtggtt tgtccaaa
//
