LOCUS       sequence-322267-        2346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression and localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, 
            Mathur P, Schauer K, Goud B, Albanese V, Gautier R, Subra M, Kovacs 
            D, Barelli H, Antonny B
  TITLE     A comprehensive library of fluorescent constructs of SARS-CoV-2 
            proteins and their initial characterisation in different cell types.
  JOURNAL   Biol Cell. 2021 Jul;113(7):311-328. doi: 10.1111/boc.202000158. Epub
            2021 May 10.
  PUBMED    33666950
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/boc.202000158"; journalName: "Biol Cell"; date: "2021-07"; 
            volume: "113"; issue: "7"; pages: "311-328"
COMMENT     Sequence Label: Nsp4-mCherry
FEATURES             Location/Qualifiers
     source          1..2346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..39
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    1575..1627
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1629..1638
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1780..1798)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2049..2068
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
       61 cgctagccac catgaagatt gtaaacaatt ggcttaaaca acttattaaa gtaaccctgg
      121 tattcttgtt tgtcgccgca atcttttact tgataacacc ggtccacgtg atgtctaaac
      181 acacagactt tagtagtgag ataatcggat ataaagctat cgatggcggc gttacccgcg
      241 acatcgcttc aaccgacaca tgtttcgcta ataagcacgc cgattttgac acttggttta
      301 gtcaaagagg aggaagctac acaaacgaca aggcttgtcc gcttatcgcg gctgtaataa
      361 caagggaagt tgggtttgtc gtgcctggtt tgccaggtac tatactcaga accacaaacg
      421 gtgactttct gcacttcttg ccaagggttt ttagcgcggt aggcaatatc tgctatacgc
      481 caagcaagct gattgaatac accgactttg cgacgagcgc atgtgtcctc gctgcagagt
      541 gtacgatatt taaggatgcg agcggaaagc ccgtacctta ctgctatgat actaatgtcc
      601 ttgagggatc agttgcgtat gagagtctta ggccggatac acgatatgtg ctcatggatg
      661 gttccataat ccagttccca aatacctacc tcgaagggtc cgttcgggtc gtcaccacat
      721 ttgatagcga atattgcaga cacggaacat gcgaacggag tgaagcaggg gtctgcgtct
      781 ccacaagtgg gcggtgggtc ctcaataacg attattaccg gagcctgccc ggcgtgtttt
      841 gcggggtcga tgcggttaac ttgttgacga atatgtttac gcctctcata caacccattg
      901 gcgcactgga tatatctgca agtatagtcg ctggaggcat tgtggcaatc gtggttacgt
      961 gtttggcgta ttattttatg aggttccgcc gggctttcgg ggaatattcc cacgtagtcg
     1021 ccttcaacac actcttgttt ctcatgtctt tcactgtact gtgtttgact cctgtctact
     1081 ctttcttgcc gggtgtatac tcagttattt acttgtacct cacgttttat ttgacaaacg
     1141 atgtttcatt tctcgcgcat atccaatgga tggtgatgtt tacgccattg gtgcctttct
     1201 ggatcacgat cgcttatatc atttgcatca gcaccaaaca cttctattgg ttctttagta
     1261 actacttgaa gcgcagggtt gtttttaacg gtgtcagctt ctcaacgttt gaagaggcag
     1321 cactgtgtac tttccttctg aataaggaaa tgtatctcaa gctgcggtcc gatgtcctgc
     1381 ttccgcttac ccagtataat cgctatctcg ccctgtataa taagtacaag tacttctcag
     1441 gcgcgatgga tacaaccagt tatagagagg ctgcctgctg ccatcttgcc aaagctttga
     1501 atgacttcag caatagtggc tcagacgttc tctaccaacc accgcaaact tccataacct
     1561 cagccgtact ccagctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc
     1621 gggatcccgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     1681 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     1741 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     1801 gtggccccct gccctttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     1861 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     1921 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     1981 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     2041 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     2101 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     2161 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     2221 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     2281 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     2341 aagtag
//
