LOCUS       sequence-322271-        2426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression and localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, 
            Mathur P, Schauer K, Goud B, Albanese V, Gautier R, Subra M, Kovacs 
            D, Barelli H, Antonny B
  TITLE     A comprehensive library of fluorescent constructs of SARS-CoV-2 
            proteins and their initial characterisation in different cell types.
  JOURNAL   Biol Cell. 2021 Jul;113(7):311-328. doi: 10.1111/boc.202000158. Epub
            2021 May 10.
  PUBMED    33666950
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/boc.202000158"; journalName: "Biol Cell"; date: "2021-07"; 
            volume: "113"; issue: "7"; pages: "311-328"
COMMENT     Sequence Label: Nsp14-mCherry
FEATURES             Location/Qualifiers
     source          1..2426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..39
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1656..1708
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1710..1719
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1716..2426
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1861..1879)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2129..2148
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
       61 cgctagccac catggctgaa aatgtaacgg gcttgtttaa agactgtagt aaagtgatca
      121 caggactcca ccccacacaa gcacctaccc acctttccgt agatacgaag ttcaaaacgg
      181 aaggattgtg tgtggatata ccagggatac caaaggatat gacgtaccga aggctgattt
      241 ccatgatggg ttttaagatg aattaccaag ttaatggcta ccccaacatg ttcatcacca
      301 gggaggaggc aattagacac gtaagagcct ggataggctt cgatgttgaa ggttgccatg
      361 caacccggga agccgtaggc acaaaccttc cgttgcagct tggcttttcc acgggcgtca
      421 acctcgttgc cgtaccgact ggctatgttg acacgccgaa caacaccgat ttctctcgcg
      481 taagtgctaa gcctcctccg ggagatcaat tcaagcatct tatacctctc atgtacaagg
      541 gactgccgtg gaatgtagtt cgcattaaga tcgtgcagat gctctccgat actttgaaaa
      601 atctgagcga cagggtggtc ttcgttctgt gggcacatgg cttcgaactt acttctatga
      661 aatactttgt aaaaatcggg cccgagcgga catgttgttt gtgcgaccga cgagctactt
      721 gttttagcac agcatctgac acctacgcat gctggcacca cagtataggc ttcgattacg
      781 tctacaatcc ctttatgata gacgtacaac aatggggctt tacgggtaac ttgcagagta
      841 atcacgatct ttactgccaa gttcatggga acgcacacgt ggcctcctgc gatgcgataa
      901 tgacgaggtg cttggccgtg cacgagtgct ttgttaagcg ggtcgattgg actatagagt
      961 atcccataat cggtgacgaa cttaaaatta atgctgcatg caggaaagtg cagcacatgg
     1021 tagtaaaagc agcgctcctc gcggataagt ttcctgtgct ccacgatatc ggcaacccta
     1081 aggccattaa atgcgtcccc caagctgatg tagagtggaa attctatgac gctcagccat
     1141 gtagtgacaa agcatacaag attgaagaat tgttttattc atatgcgaca cactccgata
     1201 agttcactga cggagtttgt ctcttttgga actgtaatgt tgataggtat ccggcgaata
     1261 gcatagtgtg tcgatttgac acgcgcgttc tctctaattt gaacctccct ggctgcgacg
     1321 gggggtcttt gtacgtaaac aagcatgcat ttcatacccc agcttttgac aaatccgctt
     1381 tcgtaaatct gaaacaactt cccttcttct attattcaga tagcccttgc gaatctcacg
     1441 gtaagcaagt ggttagtgac attgactatg taccacttaa aagtgcaacg tgcattacac
     1501 gatgcaattt ggggggcgcg gtttgccgac accatgccaa cgaatacaga ttgtacctgg
     1561 atgcatataa catgatgata agtgcagggt ttagtctttg ggtgtataag cagttcgata
     1621 cgtataatct ctggaacaca tttacccgat tgcagctcga gctcaagctt cgaattctgc
     1681 agtcgacggt accgcgggcc cgggatcccg ccaccatggt gagcaagggc gaggaggata
     1741 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     1801 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     1861 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     1921 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     1981 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2041 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2101 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2161 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     2221 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     2281 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     2341 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     2401 gcggcatgga cgagctgtac aagtag
//
