LOCUS       sequence-322273-        2356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression and localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, 
            Mathur P, Schauer K, Goud B, Albanese V, Gautier R, Subra M, Kovacs 
            D, Barelli H, Antonny B
  TITLE     A comprehensive library of fluorescent constructs of SARS-CoV-2 
            proteins and their initial characterisation in different cell types.
  JOURNAL   Biol Cell. 2021 Jul;113(7):311-328. doi: 10.1111/boc.202000158. Epub
            2021 May 10.
  PUBMED    33666950
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/boc.202000158"; journalName: "Biol Cell"; date: "2021-07"; 
            volume: "113"; issue: "7"; pages: "311-328"
COMMENT     Sequence Label: Nsp14D-mCherry
FEATURES             Location/Qualifiers
     source          1..2356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1586..1638
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1640..1649
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1646..2356
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1791..1809)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2059..2078
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
       61 caccatgcaa gcacctaccc acctttccgt agatacgaag ttcaaaacgg aaggattgtg
      121 tgtggatata ccagggatac caaaggatat gacgtaccga aggctgattt ccatgatggg
      181 ttttaagatg aattaccaag ttaatggcta ccccaacatg ttcatcacca gggaggaggc
      241 aattagacac gtaagagcct ggataggctt cgatgttgaa ggttgccatg caacccggga
      301 agccgtaggc acaaaccttc cgttgcagct tggcttttcc acgggcgtca acctcgttgc
      361 cgtaccgact ggctatgttg acacgccgaa caacaccgat ttctctcgcg taagtgctaa
      421 gcctcctccg ggagatcaat tcaagcatct tatacctctc atgtacaagg gactgccgtg
      481 gaatgtagtt cgcattaaga tcgtgcagat gctctccgat actttgaaaa atctgagcga
      541 cagggtggtc ttcgttctgt gggcacatgg cttcgaactt acttctatga aatactttgt
      601 aaaaatcggg cccgagcgga catgttgttt gtgcgaccga cgagctactt gttttagcac
      661 agcatctgac acctacgcat gctggcacca cagtataggc ttcgattacg tctacaatcc
      721 ctttatgata gacgtacaac aatggggctt tacgggtaac ttgcagagta atcacgatct
      781 ttactgccaa gttcatggga acgcacacgt ggcctcctgc gatgcgataa tgacgaggtg
      841 cttggccgtg cacgagtgct ttgttaagcg ggtcgattgg actatagagt atcccataat
      901 cggtgacgaa cttaaaatta atgctgcatg caggaaagtg cagcacatgg tagtaaaagc
      961 agcgctcctc gcggataagt ttcctgtgct ccacgatatc ggcaacccta aggccattaa
     1021 atgcgtcccc caagctgatg tagagtggaa attctatgac gctcagccat gtagtgacaa
     1081 agcatacaag attgaagaat tgttttattc atatgcgaca cactccgata agttcactga
     1141 cggagtttgt ctcttttgga actgtaatgt tgataggtat ccggcgaata gcatagtgtg
     1201 tcgatttgac acgcgcgttc tctctaattt gaacctccct ggctgcgacg gggggtcttt
     1261 gtacgtaaac aagcatgcat ttcatacccc agcttttgac aaatccgctt tcgtaaatct
     1321 gaaacaactt cccttcttct attattcaga tagcccttgc gaatctcacg gtaagcaagt
     1381 ggttagtgac attgactatg taccacttaa aagtgcaacg tgcattacac gatgcaattt
     1441 ggggggcgcg gtttgccgac accatgccaa cgaatacaga ttgtacctgg atgcatataa
     1501 catgatgata agtgcagggt ttagtctttg ggtgtataag cagttcgata cgtataatct
     1561 ctggaacaca tttacccgat tgcagctcga gctcaagctt cgaattctgc agtcgacggt
     1621 accgcgggcc cgggatcccg ccaccatggt gagcaagggc gaggaggata acatggccat
     1681 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     1741 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     1801 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     1861 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     1921 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     1981 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     2041 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     2101 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     2161 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     2221 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     2281 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     2341 cgagctgtac aagtag
//
