LOCUS       sequence-322301-        6033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre-dependant expression of soma-localized Voltron in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Abdelfattah AS, Kawashima T, Singh A, Novak O, Liu H, Shuai Y, Huang
            YC, Campagnola L, Seeman SC, Yu J, Zheng J, Grimm JB, Patel R, 
            Friedrich J, Mensh BD, Paninski L, Macklin JJ, Murphy GJ, Podgorski 
            K, Lin BJ, Chen TW, Turner GC, Liu Z, Koyama M, Svoboda K, Ahrens 
            MB, Lavis LD, Schreiter ER
  TITLE     Bright and photostable chemigenetic indicators for extended in vivo 
            voltage imaging.
  JOURNAL   Science. 2019 Aug 1. pii: science.aav6416. doi: 
            10.1126/science.aav6416.
  PUBMED    31371562
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2019 Aug 1. pii: science.aav6416. doi: 10.1126/science.aav6416."
COMMENT     Sequence Label: pAAV-hsyn-flex-Voltron-ST
FEATURES             Location/Qualifiers
     source          1..6033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   157..286
                     /label=AAV2 ITR
     promoter        368..815
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    845..878
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    941..974
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      2835..2844
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2855..2888)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2959..2992)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3034..3622
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3087..3107)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3505..3516)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3625..3641)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3626..3642)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     rep_origin      complement(3913..4501)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3993..4012)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4672..5463,5464..5532))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5295..5314
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5533..5637)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(5663..6033,1..85))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5800..5821)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6012..6031
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
       61 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
      121 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgc gcgctcgctc gctcactgag
      181 gccgcccggg caaagcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag
      241 cgagcgcgca gagagggagt ggccaactcc atcactaggg gttccttgta gttaatgatt
      301 aacccgccat gctacttatc tacgtagcca tgctctagga agatctctgc agagggccct
      361 gcgtatgagt gcaagtgggt tttaggacca ggatgaggcg gggtgggggt gcctacctga
      421 cgaccgaccc cgacccactg gacaagcacc caacccccat tccccaaatt gcgcatcccc
      481 tatcagagag ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg ccagcttcag
      541 caccgcggac agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc ctcagcactg
      601 aaggcgcgct gacgtcactc gccggtcccc cgcaaactcc ccttcccggc caccttggtc
      661 gcgtccgcgc cgccgccggc ccagccggac cgcaccacgc gaggcgcgag ataggggggc
      721 acgggcgcga ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag tctgcggtgg
      781 gcagcggagg agtcgtgtcg tgcctgagag cgcagtcgaa ttcaagctgc tagcaaggat
      841 ccccataact tcgtataaag tatcctatac gaagttatat caaaatagga agaccaatgc
      901 ttcaccatcg acccgaattg ccaagcatca ccatcgaccc ataacttcgt ataatgtatg
      961 ctatacgaag ttatactaga gcttttagaa tcgagtagcc tcggggaaat ctgttgcgca
     1021 agagatgaag ctatcaatag atgacatact cctcatgtca atcactcctt cagtcctggc
     1081 agggagggga gccacgggac ttggcatact ggacatctca agctcttcgg gaggcttaga
     1141 ctgtggagcc atttcctttg tgttcaggat aggctgactt tgcacctcgt tctcgtagca
     1201 gaacacgttg atgtcgatct ggtccagggg gatgtactcg ccctcgctgg tgatcctgct
     1261 cttagtggtt ggctcgccgg aaatctcgag cgtcgacagc cagcgcgcga tctcgctgcc
     1321 gatcaggtcc gggttgtctt cttgcagcag attcagaccc gggccgatgt ccacagcctt
     1381 gcagttaggc aggcttttgg ccaggcgagc ggcttcggcc ggtgggatca gaacgcctgg
     1441 ggtgccccag aacagcagct tcgggacagg ggactggtgc agccagtcca tgtattcttc
     1501 gaccagcgcg acgatgttcg ctggctcacc ggcgattggc agctcgtttg ggaagcgcca
     1561 cagtggctcg cggtcaacag gattcaggaa cggctcgcgg taatggtcca tctcgacttc
     1621 agtcagcggg cggacgacac ccatcggcag cgtaccctcg ataaaaacgt tctgatcgat
     1681 gatcagcttg cggccgacgt cggtggtgcg gaaggcctgg aaggtctcgc gggcaaattc
     1741 tggccattcg tcccaggtcg ggatagggcg gatgaactcc ataaatgcaa tacctttgac
     1801 gcgctctgga ttgcgcttgg cccagtggaa acccagagcg gagccccagt cgtgaatgac
     1861 caggacgacc tcttccagac ccagggcttc gatgaaggca tccatgaagc ggacgtggtc
     1921 gtcgaagaaa taacccaggt ctggtttgtc ggatttgccc ataccgatca ggtctggagc
     1981 aatgcagcga tgggtcggtg caacatgcgg gatgatgttg cgccacacgt aggaggaggt
     2041 cgggttaccg tgcaggaaca gcacaggggt gccatcgcgc ggaccaacat cgacgtagtg
     2101 catgcgctcg cccaggactt ccacataatg ggggtcgaat ggaaagccag taccgatctt
     2161 gaagatagtc tcatgggcaa tgaggcaccc cattccaaac agtggcttag cgagcacatc
     2221 caggattccc atgagaatgg cctcattgtc gacggacagc acgccactac cagacccgaa
     2281 agcccacacg acaggataaa acacccagag gattgacagg aagacgagca gactgacata
     2341 ggagctatgg tactcggggc ttttcttcag atcgtctttc caagtggcgt tatacatccc
     2401 gtatgtcagg actgcctgca tcacacatcc agcgataaag tacacccatt tgaacttgtg
     2461 ttcatcctcg aaggcgccca gaataccaaa cacgatcatg aaaatgtctg cgccgatgac
     2521 ccaagaaatc atcactccgc ccatcttggt catgacgatg agatcgagca ggagcagtgg
     2581 tgtggtcagc acccagttaa tatatcttgc gtagaccacc tgcctttcac cattcattgt
     2641 caggtcctgg tagttcacgg ccattgcata gtaagcggta gcggcgatag tgcagatggc
     2701 aatgttgata tagtatgcgc tcttcagctc tgaaggccga aagtgcacag ccactccaaa
     2761 agcaatagca gcagcagcca taatagcaaa gacaatccac tgtgcaatca tgccggtctc
     2821 ggtttccacg tcagccatgg tggcggatcc tagtataact tcgtatagga tactttatac
     2881 gaagttatca ttgggattct tcctattttg atccaagcat caccatcgac cctctagtcc
     2941 agatctcacc atcgacccat aacttcgtat agcatacatt atacgaagtt atgtccctcg
     3001 aagaggttcg aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     3061 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3121 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3181 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3241 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3301 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3361 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3421 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     3481 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3541 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3601 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gacccgggcg gcctcagtga
     3661 gcgagcgagc gcgcagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3721 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3781 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3841 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3901 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3961 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4021 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4081 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4141 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4201 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4261 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4321 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4381 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4441 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4501 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4561 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4621 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4681 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4741 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4801 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4861 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4921 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4981 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     5041 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     5101 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     5161 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5221 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     5281 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5341 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5401 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5461 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5521 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5581 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5641 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     5701 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     5761 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     5821 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     5881 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     5941 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     6001 gcgagaaagg aagggaagaa agcgaaagga gcg
//
