LOCUS       sequence-322302-       10453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of hyA3A-BE4max.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Zhang X, Chen L, Zhu B, Wang L, Chen C, Hong M, Huang Y, Li H, Han 
            H, Cai B, Yu W, Yin S, Yang L, Yang Z, Liu M, Zhang Y, Mao Z, Wu Y, 
            Liu M, Li D
  TITLE     Increasing the efficiency and targeting range of cytidine base 
            editors through fusion of a single-stranded DNA-binding protein 
            domain.
  JOURNAL   Nat Cell Biol. 2020 Jun;22(6):740-750. doi: 
            10.1038/s41556-020-0518-8. Epub 2020 May 11.
  PUBMED    32393889
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-020-0518-8"; journalName: "Nat Cell Biol"; date: 
            "2020-06"; volume: "22"; issue: "6"; pages: "740-750"
COMMENT     Sequence Label: hyA3A-BE4max
FEATURES             Location/Qualifiers
     source          1..10453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..98)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        290..538
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     290..310
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     461..480
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        557..632
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        652..1031
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1032..1235
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1186..1206
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1317..1337
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2466..2489,2490..2492,2493..6566)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2466..6566
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6597..6845
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6876..7124
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7167..7187
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7197..7253
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7254..7256,7257..7259,7260..7970)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7254..7256,7257..7259,7260..7970)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7560..7579)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7907..7928
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7983..8003
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(8026..8043)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8032..8256
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8340..8362)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8351..8367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8375..8381,8382..8399,8400..8405))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8420..8441
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8558..8575)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8729..9317)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8809..8828)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9488..10279,10280..10348))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10111..10130
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10349..10453)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat ctcccgatcc
       61 cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
      121 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
      181 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      241 tcgcgatgta cgggccagat atacgcgtgg atccctgcag ttaattaaag agggcctatt
      301 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
      361 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      421 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      481 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
      541 gtcttcgaga agacctgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
      601 atcaacttga aaaagtggca ccgagtcggt gcttttttta tgcatggtac cgacattgat
      661 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      721 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      781 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      841 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      901 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      961 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     1021 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     1081 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     1141 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     1201 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
     1261 agagatccgc ggccgccacc atgaagagga ccgccgatgg ctctgagttc gagagcccca
     1321 agaagaagcg gaaggtggag gcatctccag caagcggacc aaggcacctg atggaccccc
     1381 acatcttcac ctctaacttt aacaatggca tcggcaggca caagacatac ctgtgctatg
     1441 aggtggagcg cctggacaac ggcaccagcg tgaagatgga tcagcacaga ggcttcctgc
     1501 acaaccaggc caagaatctg ctgtgcggct tctacggccg gcacgcagag ctgagatttc
     1561 tggacctggt gcctagcctg cagctggatc cagcccagat ctatagggtg acctggttca
     1621 tcagctggtc cccatgcttt tcctggggat gtgcaggaga ggtgcgcgcc ttcctgcagg
     1681 agaatacaca cgtgcggctg agaatctttg ccgcccggat ctacgactat gatcctctgt
     1741 acaaggaggc cctgcagatg ctgagagacg caggagccca ggtgtccatc atgacctatg
     1801 atgagttcaa gcactgctgg gacacatttg tggatcacca gggctgtccc tttcagcctt
     1861 gggacggact ggatgagcac tcccaggccc tgtctggcag gctgagggcc atcctgcaga
     1921 accagggcaa tagcggagga tctagcggag gatcaagcgg aagcgagact cctggaacca
     1981 gcgaaagcgc aaccccagaa agcagcggag gaagtagcgg aggaagcatg gcaatgcaga
     2041 tgcagcttga agcaaatgca gatacttcag tggaagaaga aagctttggc ccacaaccca
     2101 tttcacggtt agagcagtgt ggcataaatg ccaacgatgt gaagaaattg gaagaagctg
     2161 gattccatac tgtggaggct gttgcctatg cgccaaagaa ggagctaata aatattaagg
     2221 gaattagtga agccaaagct gataaaattc tggctgaggc agctaaatta gttccaatgg
     2281 gtttcaccac tgcaactgaa ttccaccaaa ggcggtcaga gatcatacag attactactg
     2341 gctccaaaga gcttgacaaa ctacttcaat ctggcggatc tagcggtgga tctagcggct
     2401 ctgagacccc tggaacatcc gaatccgcca ctccagagag cagcggaggc tcttctggag
     2461 gatcagacaa gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg
     2521 tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc
     2581 ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg
     2641 aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct
     2701 gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca
     2761 gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg
     2821 gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa
     2881 agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc
     2941 acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg
     3001 acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc
     3061 ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca
     3121 gacggctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa
     3181 acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg
     3241 aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg
     3301 cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca
     3361 tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct
     3421 ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc
     3481 ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg
     3541 ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc
     3601 tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc
     3661 ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc
     3721 acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga
     3781 tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca
     3841 gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg
     3901 aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata
     3961 agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg
     4021 tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc
     4081 tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga
     4141 ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa
     4201 tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa
     4261 ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg
     4321 tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg
     4381 cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg
     4441 gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc
     4501 tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg
     4561 acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc
     4621 tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga
     4681 cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg
     4741 tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga
     4801 gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc
     4861 ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc
     4921 gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc
     4981 atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa
     5041 gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga
     5101 agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc
     5161 tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac
     5221 agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga
     5281 acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt
     5341 ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca
     5401 actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa
     5461 agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga
     5521 agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca
     5581 gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc
     5641 ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt
     5701 ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg
     5761 tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga
     5821 tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg
     5881 cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg
     5941 tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg
     6001 actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta
     6061 agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac
     6121 tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca
     6181 gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg
     6241 aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag
     6301 tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata
     6361 agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag
     6421 cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca
     6481 aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga
     6541 tcgacctgtc tcagctggga ggtgacagcg gcgggagcgg cgggagcggg gggagcacta
     6601 atctgagcga catcattgag aaggagactg ggaaacagct ggtcattcag gagtccatcc
     6661 tgatgctgcc tgaggaggtg gaggaagtga tcggcaacaa gccagagtct gacatcctgg
     6721 tgcacaccgc ctacgacgag tccacagatg agaatgtgat gctgctgacc tctgacgccc
     6781 ccgagtataa gccttgggcc ctggtcatcc aggattctaa cggcgagaat aagatcaaga
     6841 tgctgagcgg aggatccgga ggatctggag gcagcaccaa cctgtctgac atcatcgaga
     6901 aggagacagg caagcagctg gtcatccagg agagcatcct gatgctgccc gaagaagtcg
     6961 aagaagtgat cggaaacaag cctgagagcg atatcctggt ccataccgcc tacgacgaga
     7021 gtaccgacga aaatgtgatg ctgctgacat ccgacgcccc agagtataag ccctgggctc
     7081 tggtcatcca ggattccaac ggagagaaca aaatcaaaat gctgtctggc ggctcaaaaa
     7141 gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtcgga agcggagcta
     7201 ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga cctatggtga
     7261 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     7321 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     7381 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     7441 ccaccctgac ctatggagtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     7501 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     7561 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     7621 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     7681 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     7741 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     7801 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     7861 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     7921 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tctggtggtt
     7981 ctcccaagaa gaaaaggaaa gtctaagttt aaacccgctg atcagcctcg actgtgcctt
     8041 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     8101 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     8161 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga
     8221 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     8281 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagatgtttc
     8341 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8401 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8461 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8521 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8581 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8641 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8701 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8761 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8821 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8881 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8941 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9001 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9061 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9121 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9181 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9241 gcaaacaaac caccgctagt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9301 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9361 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9421 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9481 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9541 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9601 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9661 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9721 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9781 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9841 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9901 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9961 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10021 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10081 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10141 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10201 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10261 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10321 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10381 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10441 taggggttcc gcg
//
