LOCUS       sequence-322303-       11137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fluorescent ALIX S718/721A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11137)
  TITLE     Plasmids for creating inducible cell lines expressing ALIX-GFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVX ALIX-GFP S718/721A
FEATURES             Location/Qualifiers
     source          1..11137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2206..2520
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2214..2232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2250..2268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2285..2303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2321..2339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2357..2375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2392..2410
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2428..2446
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2493..2517
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(5237..5239,5240..5242,5243..5956)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5237..5239,5240..5242,5243..5953)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5282..5303)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5543..5562)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5890..5911
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5970..6469
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6005..6028)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6402..6421
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6490..7089
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6490..6509)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6986..7006
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7106..7694
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7159..7179)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7577..7588)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7901..8534
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8662..8678)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8662..8678)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8675..8697)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8686..8702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8710..8716,8717..8734,8735..8740))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8755..8776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8893..8910)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9064..9652)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9144..9163)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9823..10614,10615..10683))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10446..10465
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10684..10788)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10836..10970
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10873..10892)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10927..10946
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2521 tggagaagga tccgccacca tgatggcgac attcatctcg gtgcagctga aaaagacctc
     2581 agaggtggac ctggccaagc cgctggtgaa gttcatccag cagacttacc caagcggcgg
     2641 ggaagagcag gcccagtact gccgcgcggc ggaggagctc agcaagctgc gccgcgccgc
     2701 agtcggtcgt ccgctggaca agcacgaggg cgcgctcgag acgctcctga gatattatga
     2761 tcagatttgt tctattgaac ccaaattccc attttctgaa aatcagatct gcttgacatt
     2821 tacctggaag gatgctttcg ataaaggttc actttttgga ggctctgtaa aactggctct
     2881 tgcaagctta ggatatgaaa agagctgtgt gttgttcaat tgtgcagcct tagctagcca
     2941 aattgcagca gaacagaact tagacaacga tgaaggattg aaaatcgctg ctaaacatta
     3001 ccagtttgct agtggtgcct ttttacatat taaagagacg gttttatctg ccttaagtcg
     3061 agagccgacc gtggacatat ctccagatac tgttgggacc ctcagtctta ttatgctggc
     3121 acaggctcaa gaagtatttt ttttaaaagc cacaagagat aaaatgaaag atgccatcat
     3181 agctaaattg gctaatcagg ctgcagatta ttttggtgat gctttcaaac agtgtcaata
     3241 caaagatact ctccccaagg aggtgttccc tgtcttggct gcaaagcact gtatcatgca
     3301 ggccaatgct gagtaccatc agtctatcct ggcaaaacag cagaagaaat ttggagaaga
     3361 aattgcaagg ttacagcatg cagcagaact gattaaaaca gtggcatctc gctatgatga
     3421 atatgttaat gtgaaggatt tttctgacaa aatcaatcgt gcccttgctg cagcaaagaa
     3481 ggataatgac ttcatttatc atgatcgagt tccagacctt aaagatctag atcctattgg
     3541 caaagccaca cttgtgaaat ctaccccggt caatgtaccc atcagtcaga aatttactga
     3601 tctgtttgag aagatggttc ccgtgtcagt acagcagtct ttggctgcct ataatcagag
     3661 gaaagccgat ttggttaaca gatcaattgc tcagatgaga gaagccacca ctttggcaaa
     3721 tggggtgcta gcttccctta atcttccagc agcaattgaa gatgtgtctg gagacactgt
     3781 acctcagtct atattgacta aatccagatc tgtgattgaa cagggaggca tccagactgt
     3841 tgatcagttg attaaagaac tgcctgaatt actgcaacga aatagagaaa tcctagatga
     3901 gtcattaagg ttgttggatg aagaagaagc aaccgataat gatttaagag caaaatttaa
     3961 ggaacgttgg caaaggacac catccaatga actgtataag cctttaagag cagagggaac
     4021 caacttcaga acagttttag ataaagctgt gcaggcagat ggacaagtga aagaatgtta
     4081 ccagtctcat cgtgacacca tcgtgctttt gtgtaagcca gagcctgagc tgaatgctgc
     4141 catcccttct gctaatccag caaagaccat gcagggcagt gaggttgtaa atgtcttaaa
     4201 atccttattg tcaaatcttg atgaagtaaa gaaggaaaga gagggtctgg agaatgactt
     4261 gaaatctgtg aattttgaca tgacaagcaa gtttttgaca gccctggctc aagatggtgt
     4321 gataaatgaa gaagctcttt ctgttactga actagatcga gtctatggag gtcttacaac
     4381 taaagtccaa gaatctctaa agaaacagga gggacttctt aaaaatattc aggtctcaca
     4441 tcaggaattt tcaaaaatga aacaatctaa taatgaagct aacttaagag aagaagtttt
     4501 gaagaattta gctactgcgt atgacaactt tgttgaactt gtagctaatt tgaaggaagg
     4561 cacaaagttt tacaatgagt tgactgaaat cctggtcagg ttccagaaca aatgcagtga
     4621 tatagttttt gcacggaaga cagaaagaga tgaactctta aaggacttgc aacaaagcat
     4681 tgccagagaa cctgctgctc ctgctattcc tacacctgcg tatcagtcct caccagcagg
     4741 aggacatgca ccaactcctc caactccagc gccaagaacc atgccgccta ctaagcccca
     4801 gcccccagcc aggcctccac cacctgtgct tccagcaaat cgagctcctt ctgctactgc
     4861 tccatctcca gtgggggctg ggactgctgc gccagctcca tcacaaacgc ctggctcagc
     4921 tcctcctcca caggcgcagg gaccacccta tcccacctat ccaggatatc ctgggtattg
     4981 ccaaatgccc atgcccatgg gctataatcc ttatgcgtat ggccagtata atatgccata
     5041 tccaccagtg tatcaccaga gtcctggaca ggctccatac ccgggacccc agcagccttc
     5101 ataccccttc cctcagcccc cacagcagtc ttactatcca cagcagcccg gggacgagga
     5161 gctcgagcgc cggcttcgcg aggaattgga acggcgcctt cgcgaggagc tggaacgtcg
     5221 actccgcggc tcttccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     5281 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     5341 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     5401 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     5461 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     5521 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     5581 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     5641 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     5701 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     5761 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     5821 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     5881 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5941 cgagctgtac aagtaataag aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     6001 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
     6061 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
     6121 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
     6181 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
     6241 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
     6301 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
     6361 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
     6421 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
     6481 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6541 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     6601 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     6661 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6721 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6781 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6841 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     6901 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     6961 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     7021 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     7081 ggtgcctgac gggcgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt
     7141 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     7201 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     7261 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     7321 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7381 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7441 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7501 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7561 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7621 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7681 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc
     7741 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     7801 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     7861 gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc
     7921 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     7981 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     8041 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
     8101 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
     8161 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
     8221 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
     8281 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
     8341 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8401 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8461 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8521 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8581 gaaatgaata tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag
     8641 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8701 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8761 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8821 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8881 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8941 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9001 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9061 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9121 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9181 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9241 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9301 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9361 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9421 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9481 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9541 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9601 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9661 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9721 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9781 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9841 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9901 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9961 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10021 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10081 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10141 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10201 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10261 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10321 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10381 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10441 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10501 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10561 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10621 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10681 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10741 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10801 aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg
    10861 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10921 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    10981 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    11041 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    11101 attgtatttg ttaaatatgt actacaaact tagtagt
//
