LOCUS       sequence-322304-        7937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human immunoglobulin gamma 1 antibody expression vector (kappa light
            chain) without variable regions. Enables Colony-PCR screening of 
            false-positive (PCR vector template) colonies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, 
            Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
  TITLE     A tool kit for rapid cloning and expression of recombinant 
            antibodies.
  JOURNAL   Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
  PUBMED    25073855
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-07-30"; volume: "4:5885. doi"; pages: " 
            10.1038/srep05885"
COMMENT     Sequence Label: pVITRO1-dV-IgG1/kappa
FEATURES             Location/Qualifiers
     source          1..7937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1405..1526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1416..1435)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1470..1489
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1714..2302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2203..2222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2382..2685
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2800..4105
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    3142..4073
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     misc_feature    4529..4973
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        5006..5052
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        5044..5062
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5072..6097
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6109..6681
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     enhancer        6733..6924
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6756..6776)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(6938..7937,1..306)
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          join(7265..7937,1..274)
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
ORIGIN
        1 aaggaagagg gcctggtccc tcaccggctg ctgcttcctg tgaccccgtg gtcctatcgg
       61 ccgcaatagt cacctcgggc ttttgagcac ggctagtcgc ggcgggggga ggggatgtaa
      121 tggcgttgga gtttgttcac atttggtggg tggagactag tcaggccagc ctggcgctgg
      181 aagtcatttt tggaatttgt ccccttgagt tttgagcgga gctaattctc gggcttctta
      241 gcggttcaaa ggtatctttt aaaccctttt ttaggtgttg tgaaaaccac cgctaattca
      301 aagcaaccgg tatggactgg acctggagga tcctcttctt ggtggcggcc gccacaggcg
      361 cgcactccga attcgatatc ctcgaggcta gcaccaaggg cccatcggtc ttccccctgg
      421 caccctcctc caagagcacc tctgggggca cagcggccct gggctgcctg gtcaaggact
      481 acttccccga accggtgacg gtgtcgtgga actcaggcgc cctgaccagc ggcgtgcaca
      541 ccttcccggc tgtcctacag tcctcaggac tctactccct cagcagcgtg gtgaccgtgc
      601 cctccagcag cttgggcacc cagacctaca tctgcaacgt gaatcacaag cccagcaaca
      661 ccaaggtgga caagaaagtt gagcccaaat cttgtgacaa aactcacaca tgcccaccgt
      721 gcccagcacc tgaactcctg gggggaccgt cagtcttcct cttcccccca aaacccaagg
      781 acaccctcat gatctcccgg acccctgagg tcacatgcgt ggtggtggac gtgagccacg
      841 aagaccctga ggtcaagttc aactggtacg tggacggcgt ggaggtgcat aatgccaaga
      901 caaagccgcg ggaggagcag tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc
      961 tgcaccagga ctggctgaat ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc
     1021 cagcccccat cgagaaaacc atctccaaag ccaaagggca gccccgagaa ccacaggtgt
     1081 acaccctgcc cccatcccgg gatgagctga ccaagaacca ggtcagcctg acctgcctgg
     1141 tcaaaggctt ctatcccagc gacatcgccg tggagtggga gagcaatggg cagccggaga
     1201 acaactacaa gaccacgcct cccgtgctgg actccgacgg ctccttcttc ctctacagca
     1261 agctcaccgt ggacaagagc aggtggcagc aggggaacgt cttctcatgc tccgtgatgc
     1321 atgaggctct gcacaaccac tacacgcaga agagcctctc cctgtctccg ggtaaatgat
     1381 gtacagctag ctggccagac atgataagat acattgatga gtttggacaa accacaacta
     1441 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     1501 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     1561 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     1621 aaatgttaat taactagcca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1681 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1741 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1801 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1861 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1921 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1981 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2041 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2101 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2161 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2221 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2281 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2341 ttgctggcct tttgctcaca tgttcttaat taacctgcag gcgttacata acttacggta
     2401 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2461 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2521 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2581 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2641 cctacttggc agtacatcta cgtattagtc atcgctatta ccatgatgat gcggttttgg
     2701 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     2761 attgacgtca atgggagttt gttttgacta gtggagccga gagtaattca tacaaaagga
     2821 gggatcgcct tcgcaagggg agagcccagg gaccgtccct aaattctcac agacccaaat
     2881 ccctgtagcc gccccacgac agcgcgagga gcatgcgccc agggctgagc gcgggtagat
     2941 cagagcacac aagctcacag tccccggcgg tggggggagg ggcgcgctga gcgggggcca
     3001 gggagctggc gcggggcaaa ctgggaaagt ggtgtcgtgt gctggctccg ccctcttccc
     3061 gagggtgggg gagaacggta tataagtgcg gtagtcgcct tggacgttct ttttcgcaac
     3121 gggtttgccg tcagaacgca ggtgagtggc gggtgtggct tccgcgggcc ccggagctgg
     3181 agccctgctc tgagcgggcc gggctgatat gcgagtgtcg tccgcagggt ttagctgtga
     3241 gcattcccac ttcgagtggc gggcggtgcg ggggtgagag tgcgaggcct agcggcaacc
     3301 ccgtagcctc gcctcgtgtc cggcttgagg cctagcgtgg tgtccgccgc cgcgtgccac
     3361 tccggccgca ctatgcgttt tttgtccttg ctgccctcga ttgccttcca gcagcatggg
     3421 ctaacaaagg gagggtgtgg ggctcactct taaggagccc atgaagctta cgttggatag
     3481 gaatggaagg gcaggagggg cgactggggc ccgcccgcct tcggagcaca tgtccgacgc
     3541 cacctggatg gggcgaggcc tgtggctttc cgaagcaatc gggcgtgagt ttagcctacc
     3601 tgggccatgt ggccctagca ctgggcacgg tctggcctgg cggtgccgcg ttcccttgcc
     3661 tcccaacaag ggtgaggccg tcccgcccgg caccagttgc ttgcgcggaa agatggccgc
     3721 tcccggggcc ctgttgcaag gagctcaaaa tggaggacgc ggcagcccgg tggagcgggc
     3781 gggtgagtca cccacacaaa ggaagagggc cttgcccctc gccggccgct gcttcctgtg
     3841 accccgtggt ctatcggccg catagtcacc tcgggcttct cttgagcacc gctcgtcgcg
     3901 gcggggggag gggatctaat ggcgttggag tttgttcaca tttggtgggt ggagactagt
     3961 caggccagcc tggcgctgga agtcattctt ggaatttgcc cctttgagtt tggagcgagg
     4021 ctaattctca agcctcttag cggttcaaag gtattttcta aacccgtttc caggtgttgt
     4081 gaaagccacc gctaattcaa agcaatccgg aatgttgcca tcacaactca ttgggtttct
     4141 gctgctctgg gttccagcta gccgcggtga attcgatatc ctcgagcgta cggtggcggc
     4201 gccatctgtc ttcatcttcc cgccatctga tgagcagttg aaatctggaa ctgcctctgt
     4261 tgtgtgcctg ctgaataact tctatcccag agaggccaaa gtacagtgga aggtggataa
     4321 cgccctccaa tcgggtaact cccaggagag tgtcacagag caggacagca aggacagcac
     4381 ctacagcctc agcagcaccc tgacgctgag caaagcagac tacgagaaac acaaagtcta
     4441 cgcctgcgaa gtcacccatc agggcctgag ctcgcccgtc acaaagagct tcaacagggg
     4501 agagtgttag ggatcccgta cgcctaggag caggtttccc caatgacaca aaacgtgcaa
     4561 cttgaaactc cgcctggtct ttccaggtct agaggggtaa cactttgtac tgcgtttggc
     4621 tccacgctcg atccactggc gagtgttagt aacagcactg ttgcttcgta gcggagcatg
     4681 acggccgtgg gaactcctcc ttggtaacaa ggacccacgg ggccaaaagc cacgcccaca
     4741 cgggcccgtc atgtgtgcaa ccccagcacg gcgactttac tgcgaaaccc actttaaagt
     4801 gacattgaaa ctggtaccca cacactggtg acaggctaag gatgcccttc aggtaccccg
     4861 aggtaacacg cgacactcgg gatctgagaa ggggactggg gcttctataa aagcgctcgg
     4921 tttaaaaagc ttctatgcct gaataggtga ccggaggtcg gcacctttcc tttgcaatta
     4981 ctgaccctat gaatacactg actgtttgac aattaatcat cggcatagta tatcggcata
     5041 gtataatacg actcactata ggagggccac catgaagaaa cctgaactga cagcaacttc
     5101 tgttgagaag tttctcattg aaaaatttga ttctgtttct gatctcatgc agctgtctga
     5161 aggtgaagaa agcagagcct tttcttttga tgttggagga agaggttatg ttctgagggt
     5221 caattcttgt gctgatggtt tttacaaaga cagatatgtt tacagacact ttgcctctgc
     5281 tgctctgcca attccagaag ttctggacat tggagaattt tctgaatctc tcacctactg
     5341 catcagcaga agagcacaag gagtcactct ccaggatctc cctgaaactg agctgccagc
     5401 tgttctgcaa cctgttgctg aagcaatgga tgccattgca gcagctgatc tgagccaaac
     5461 ctctggattt ggtccttttg gtccccaagg cattggtcag tacaccactt ggagggattt
     5521 catttgtgcc attgctgatc ctcatgtcta tcactggcag actgtgatgg atgacacagt
     5581 ttctgcttct gttgctcagg cactggatga actcatgctg tgggcagaag attgtcctga
     5641 agtcagacac ctggtccatg ctgattttgg aagcaacaat gttctgacag acaatggcag
     5701 aatcactgca gtcattgact ggtctgaagc catgtttgga gattctcaat atgaggttgc
     5761 caacattttt ttttggagac cttggctggc ttgcatggaa caacaaacaa gatattttga
     5821 aagaagacac ccagaactgg ctggttcccc cagactgaga gcctacatgc tcagaattgg
     5881 cctggaccaa ctgtatcaat ctctggttga tggaaacttt gatgatgctg cttgggcaca
     5941 aggaagatgt gatgccattg tgaggtctgg tgctggaact gttggaagaa ctcaaattgc
     6001 aagaaggtct gctgctgttt ggactgatgg atgtgttgaa gttctggctg actctggaaa
     6061 caggagaccc tccacaagac ccagagccaa ggaatgaata ttagctagat tatccctaat
     6121 acctgccacc ccactcttaa tcagtggtgg aagaacggtc tcagaactgt ttgtttcaat
     6181 tggccattta agtttagtag taaaagactg gttaatgata acaatgcatc gtaaaacctt
     6241 cagaaggaaa ggagaatgtt ttgtggacca ctttggtttt cttttttgcg tgtggcagtt
     6301 ttaagttatt agtttttaaa atcagtactt tttaatggaa acaacttgac caaaaatttg
     6361 tcacagaatt ttgagaccca ttaaaaaagt taaatgagaa acctgtgtgt tcctttggtc
     6421 aacaccgaga catttaggtg aaagacatct aattctggtt ttacgaatct ggaaacttct
     6481 tgaaaatgta attcttgagt taacacttct gggtggagaa tagggttgtt ttccccccac
     6541 ataattggaa ggggaaggaa tatcatttaa agctatggga gggttgcttt gattacaaca
     6601 ctggagagaa atgcagcatg ttgctgattg cctgtcacta aaacaggcca aaaactgagt
     6661 ccttgggttg catagaaagc tgcctgcagg gcctgaaata acctctgaaa gaggaacttg
     6721 gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     6781 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6841 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6901 caattagtca gcaaccatag tcccactagt ggagccgaga gtaattcata caaaaggagg
     6961 gatcgccttc gcaaggggag agcccaggga ccgtccctaa attctcacag acccaaatcc
     7021 ctgtagccgc cccacgacag cgcgaggagc atgcgctcag ggctgagcgc ggggagagca
     7081 gagcacacaa gctcatagac cctggtcgtg ggggggagga ccggggagct ggcgcggggc
     7141 aaactgggaa agcggtgtcg tgtgctggct ccgccctctt cccgagggtg ggggagaacg
     7201 gtatataagt gcggcagtcg ccttggacgt tctttttcgc aacgggtttg ccgtcagaac
     7261 gcaggtgagg ggcgggtgtg gcttccgcgg gccgccgagc tggaggtcct gctccgagcg
     7321 ggccgggccc cgctgtcgtc ggcggggatt agctgcgagc attcccgctt cgagttgcgg
     7381 gcggcgcggg aggcagagtg cgaggcctag cggcaacccc gtagcctcgc ctcgtgtccg
     7441 gcttgaggcc tagcgtggtg tccgcgccgc cgccgcgtgc tactccggcc gcactctggt
     7501 cttttttttt ttgttgttgt tgccctgctg ccttcgattg ccgttcagca ataggggcta
     7561 acaaagggag ggtgcggggc ttgctcgccc ggagcccgga gaggtcatgg ttggggagga
     7621 atggagggac aggagtggcg gctggggccc gcccgccttc ggagcacatg tccgacgcca
     7681 cctggatggg gcgaggcctg gggtttttcc cgaagcaacc aggctggggt tagcgtgccg
     7741 aggccatgtg gccccagcac ccggcacgat ctggcttggc ggcgccgcgt tgccctgcct
     7801 ccctaactag ggtgaggcca tcccgtccgg caccagttgc gtgcgtggaa agatggccgc
     7861 tcccgggccc tgttgcaagg agctcaaaat ggaggacgcg gcagcccggt ggagcgggcg
     7921 ggtgagtcac ccacaca
//
