LOCUS       sequence-322305-       10379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for the delivery of multiple sgRNAs targeting 
            different genes in combination with inducible Cas9 expresssion by 
            pLenti-iCas9-neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Cao J, Wu L, Zhang SM, Lu M, Cheung WK, Cai W, Gale M, Xu Q, Yan Q
  TITLE     An easy and efficient inducible CRISPR/Cas9 platform with improved 
            specificity for multiple gene targeting.
  JOURNAL   Nucleic Acids Res. 2016 Nov 2;44(19):e149. Epub 2016 Jul 25.
  PUBMED    27458201
REFERENCE   2  (bases 1 to 10379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-11-2"; volume: "44(19)"; pages: "e149. Epub
            2016 Jul 25"
COMMENT     Sequence Label: Lenti-multi-Guide
FEATURES             Location/Qualifiers
     source          1..10379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        130..378
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        387..462
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    518..635
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        774..1952
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1005..1943
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1900..1920
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1965..2564
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1965..1984)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2461..2481
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2580..3168
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2633..2653)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3051..3062)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3240..3473
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3551..3672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3588..3607)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3642..3661
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3712..3847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3867..3886)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3868..3886)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3896..3913)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3896..3912)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3905..3927)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4054..4509
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4141..4160)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4351..4372
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4535..4639
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4640..4708,4709..5500)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4858..4877)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5671..6259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6160..6179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6413..6430
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6547..6568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6583..6588,6589..6606,6607..6613)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6621..6637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6626..6648
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6645..6661
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6645..6661
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6680..6700
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6682..6700
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6728..6954
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6955..7135
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7182..7307
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7800..8033
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8218..8262
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8440..8688
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        join(8712..8717,8718..8735,8736..8742)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             8796..9455
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8862..8881)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             9797..10102
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9981..10000
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_RNA        10141..10216
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
       61 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
      121 ggacccagag agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
      181 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
      241 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      301 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      361 tggaaaggac gaaacaccgc gtctctgttt tagagctaga aatagcaagt taaaataagg
      421 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttaa gcttggcgta
      481 actagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
      541 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
      601 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
      661 atccactttg gcgccggctc gagggggccc gggtgcaaag atggataaag ttttaaacag
      721 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg
      781 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      841 tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      901 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      961 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     1021 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     1081 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     1141 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     1201 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     1261 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     1321 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     1381 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     1441 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     1501 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     1561 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     1621 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     1681 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     1741 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     1801 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     1861 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     1921 aaagtttttt tcttccattt caggtgtcgt gacgtacggc caccatgacc gagtacaagc
     1981 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     2041 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     2101 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     2161 tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     2221 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     2281 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     2341 ccaccgtcgg agtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     2401 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     2461 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     2521 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaacgcgt taagtcgaca
     2581 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2641 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2701 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2761 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2821 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2881 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2941 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3001 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3061 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3121 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag
     3181 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     3241 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     3301 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     3361 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     3421 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta
     3481 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     3541 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3601 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3661 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     3721 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3781 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     3841 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     3901 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     3961 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4021 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     4081 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4141 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4201 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4261 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4321 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4381 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4441 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     4501 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4561 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4621 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4681 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4741 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4801 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4861 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4921 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4981 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5041 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5101 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5161 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5221 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5281 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5341 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5401 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5461 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5521 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5581 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5641 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5701 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5761 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5821 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5881 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5941 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6001 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6061 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6121 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6181 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6241 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6301 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6361 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6421 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6481 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6541 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     6601 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     6661 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     6721 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     6781 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     6841 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     6901 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     6961 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7021 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7081 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     7141 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     7201 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     7261 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     7321 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     7381 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     7441 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     7501 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     7561 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     7621 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     7681 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     7741 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     7801 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     7861 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     7921 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     7981 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     8041 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     8101 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     8161 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     8221 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     8281 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     8341 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     8401 tcgtttcaga cccacctccc aaccccgagg ggacccagag agggcctatt tcccatgatt
     8461 ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat taatttgact
     8521 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
     8581 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
     8641 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg agacgattag
     8701 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
     8761 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     8821 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     8881 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     8941 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     9001 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     9061 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     9121 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     9181 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     9241 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     9301 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     9361 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     9421 aacagtactg cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag
     9481 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     9541 tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt
     9601 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     9661 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     9721 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     9781 aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     9841 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc
     9901 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     9961 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
    10021 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
    10081 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcagctgg
    10141 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
    10201 ggcaccgagt cggtgctttt ttaagcttgg cgtaactaga tcttgagaca aatggcagta
    10261 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagagtc
    10321 cggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattc
//
