LOCUS       sequence-322306-        9433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding ASC (for example, to assess 
            ASC-speck formation or inflammasome signalling assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif 
            H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
  TITLE     Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 
            inflammasomes.
  JOURNAL   Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y.
  PUBMED    33420033
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-8"; volume: "12(1):189. doi"; pages: " 
            10.1038/s41467-020-20320-y"
COMMENT     Sequence Label: pLEX307 ASC
FEATURES             Location/Qualifiers
     source          1..9433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             68..301
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    379..500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(416..435)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     470..489
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      540..675
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(695..714)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(696..714)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(724..741)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(724..740)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(733..755)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      882..1337
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(969..988)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1179..1200
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1363..1467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1468..1536,1537..2328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1686..1705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2499..3087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2988..3007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3241..3258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3375..3396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3411..3416,3417..3434,3435..3441)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3449..3465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3454..3476
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3473..3489
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3473..3489
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3508..3528
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3510..3528
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3556..3782
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3783..3963
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4010..4135
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4628..4861
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5046..5090
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5321..5531
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        5343..5539
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5390..5525
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5571..6599
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EC"
     CDS             5571..6599
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EC"
     misc_feature    6642..6759
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6897..8075
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7128..8066
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8023..8043
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    8095..8119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      8122..8131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8717..8741)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8749..8790
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8841..9429
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8894..8914)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9312..9323)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
       61 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
      121 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      181 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      241 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      301 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
      361 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      421 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
      481 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
      541 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
      601 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
      661 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
      721 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      781 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      841 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      901 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      961 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     1021 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     1081 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     1141 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     1201 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     1261 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     1321 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1381 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1441 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1501 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1561 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1621 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1681 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     1741 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1801 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1861 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1921 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1981 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     2041 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2101 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2161 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     2221 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     2281 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     2341 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2401 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2461 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2521 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2581 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2641 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2701 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2761 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2821 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2881 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2941 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3001 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3061 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3121 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3181 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3241 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3301 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3361 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     3421 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     3481 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     3541 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     3601 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     3661 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     3721 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     3781 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3841 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3901 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3961 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     4021 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     4081 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     4141 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     4201 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     4261 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     4321 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     4381 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     4441 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     4501 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     4561 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     4621 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     4681 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     4741 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     4801 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     4861 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     4921 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     4981 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     5041 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     5101 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     5161 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     5221 caccattatc gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa
     5281 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5341 catgcgtctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     5401 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5461 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5521 aggcctaggc ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaaaaagc
     5581 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     5641 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     5701 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     5761 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     5821 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     5881 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     5941 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     6001 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     6061 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     6121 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     6181 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     6241 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     6301 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     6361 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     6421 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     6481 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     6541 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatgttaac
     6601 gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     6661 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     6721 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     6781 agagatccac tttggcgccg gctcgaggcc tgcaggtgca aagatggata aagttttaaa
     6841 cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct
     6901 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     6961 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     7021 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     7081 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     7141 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     7201 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     7261 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
     7321 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     7381 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     7441 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     7501 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     7561 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     7621 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     7681 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     7741 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     7801 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     7861 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     7921 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     7981 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     8041 ttcaaagttt ttttcttcca tttcaggtgt cgtgaggcta gcatcgattg atcaacaagt
     8101 ttgtacaaaa aagcaggctt cgccaccatg gggcgcgcgc gcgacgccat cctggatgcg
     8161 ctggagaacc tgaccgccga ggagctcaag aagttcaagc tgaagctgct gtcggtgccg
     8221 ctgcgcgagg gctacgggcg catcccgcgg ggcgcgctgc tgtccatgga cgccttggac
     8281 ctcaccgaca agctggtcag cttttacctg gagacctacg gcgccgagct caccgctaac
     8341 gtgctgcgcg acatgggcct gcaggagatg gccgggcagc tgcaggcggc cacgcaccag
     8401 ggctctggag ccgcgccagc tgggatccag gcccctcctc agtcggcagc caagccaggc
     8461 ctgcacttta tagaccagca ccgggctgcg cttatcgcga gggtcacaaa cgttgagtgg
     8521 ctgctggatg ctctgtacgg gaaggtcctg acggatgagc agtaccaggc agtgcgggcc
     8581 gagcccacca acccaagcaa gatgcggaag ctcttcagtt tcacaccagc ctggaactgg
     8641 acctgcaagg acttgctcct ccaggcccta agggagtccc agtcctacct ggtggaggac
     8701 ctggagcgga gctgagaccc agctttcttg tacaaagtgg ttgatatcgg taagcctatc
     8761 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtaccgg ttaagtcgac
     8821 aatcaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     8881 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     8941 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     9001 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     9061 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     9121 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     9181 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     9241 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     9301 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     9361 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     9421 cctccccgcg tcg
//
