LOCUS       sequence-322307-        9094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding RFP (for example, to normalize 
            plasmid-based protein expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif 
            H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
  TITLE     Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 
            inflammasomes.
  JOURNAL   Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y.
  PUBMED    33420033
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-8"; volume: "12(1):189. doi"; pages: " 
            10.1038/s41467-020-20320-y"
COMMENT     Sequence Label: pLEX307 RFP
FEATURES             Location/Qualifiers
     source          1..9094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..212
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        24..220
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      71..206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             252..854
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(252..271)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     748..768
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    897..1014
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1152..2330
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1383..2321
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2278..2298
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2350..2374
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2380..3057
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             2380..3057
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(2510..2528)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2778..2797
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2972..2995
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(3059..3083)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3091..3132
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3183..3771
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3236..3256)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3654..3665)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3843..4076
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4154..4275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4191..4210)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4245..4264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4315..4450
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4470..4489)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4471..4489)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4499..4516)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4499..4515)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4508..4530)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4657..5112
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4744..4763)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4954..4975
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5138..5242
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5243..5311,5312..6103)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5461..5480)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6274..6862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6763..6782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7016..7033
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7150..7171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7186..7191,7192..7209,7210..7216)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7224..7240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7229..7251
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7248..7264
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7248..7264
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7283..7303
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7285..7303
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7331..7557
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7558..7738
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7785..7910
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8403..8636
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8821..8865
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agcaggcaga agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct
       61 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
      121 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
      181 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac
      241 cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
      301 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
      361 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
      421 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga
      481 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
      541 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
      601 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
      661 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
      721 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
      781 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
      841 ccggtgccag ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt
      901 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
      961 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     1021 tattacaggg acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat
     1081 ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag
     1141 gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     1201 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     1261 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     1321 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     1381 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
     1441 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
     1501 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
     1561 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
     1621 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
     1681 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
     1741 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
     1801 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
     1861 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     1921 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     1981 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     2041 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     2101 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     2161 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     2221 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     2281 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggctagcatc
     2341 gattgatcaa caagtttgta caaaaaagca ggcttcgcca tggcctcctc cgaggacgtc
     2401 atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc
     2461 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     2521 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccctca gttccagtac
     2581 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     2641 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     2701 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     2761 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
     2821 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagatgag gctgaagctg
     2881 aaggacggcg gccactacga cgccgaggtc aagaccacct acatggccaa gaagcccgtg
     2941 cagctgcccg gcgcctacaa gaccgacatc aagctggaca tcacctccca caacgaggac
     3001 tacaccatcg tggaacagta cgagcgcgcc gagggccgcc actccaccgg cgcctaagac
     3061 ccagctttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     3121 ctcgattcta cgtagtaatg aactagtacc ggttaagtcg acaatcaacg cgttaagtcg
     3181 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3241 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3301 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3361 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3421 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3481 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3541 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     3601 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3661 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3721 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt
     3781 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     3841 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     3901 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     3961 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4021 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac
     4081 gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     4141 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     4201 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     4261 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     4321 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4381 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4441 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     4501 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     4561 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     4621 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     4681 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4741 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4801 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4861 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4921 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4981 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5041 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5101 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5161 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5221 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5281 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5341 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5401 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5461 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5521 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5581 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5641 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5701 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5761 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5821 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5881 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5941 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6001 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6061 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6121 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6181 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6241 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6301 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6361 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6421 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6481 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6541 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6601 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6661 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6721 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6781 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6841 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6901 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6961 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7021 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     7081 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     7141 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     7201 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     7261 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     7321 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     7381 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     7441 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     7501 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     7561 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7621 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7681 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7741 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     7801 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     7861 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     7921 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     7981 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     8041 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     8101 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     8161 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     8221 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     8281 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     8341 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     8401 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     8461 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     8521 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     8581 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     8641 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     8701 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     8761 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     8821 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     8881 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     8941 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     9001 ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt
     9061 cagcaaccag gtgtggaaag tccccaggct cccc
//
