LOCUS       sequence-32231-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  TITLE     SGC
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAB25
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             640..657
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(710..728)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      724..771
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(895..914)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggttccgc gtggtagtga ggattataac tttgtgttca aagttgtcct
      121 gattggggag agcggcgtag gtaagacgaa tttactgtct cgtttcaccc gcaacgaatt
      181 ttcccatgac agtcgtacta cgatcggagt ggaattttcg acacgcactg ttatgttggg
      241 gacagcggcc gtcaaagcac agatatggga taccgctgga cttgaacggt acagagcgat
      301 tacgtcagcc tattaccgag gcgcagtagg tgctctcctg gtgtttgact taaccaaaca
      361 ccaaacttat gcggttgtcg agcgttggtt gaaggaactt tacgatcatg ccgaggcaac
      421 aatcgtggta atgctggttg gcaataaaag cgacctctct caggctcgcg aagtgccgac
      481 ggaagaggcg cggatgtttg ccgaaaacaa tggtctgtta ttcctggaga ccagtgcatt
      541 ggattcaact aacgtcgaac ttgcttttga aacagttctg aaagagattt tcgcgaaggt
      601 atccaaacaa tgatgacgaa gcttgcggcc gcactcgagc accaccacca ccaccactga
      661 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      721 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      781 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
      841 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      901 tcgctttctt cccttccttt ctcgccacgt tc
//
