LOCUS       sequence-322312-       13335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAVS1 targeting vector carrying CAG-SpCas9-neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13335)
  TITLE     Cas9 expression in iPSCs using AAVS1-CAGCas9neo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVS1-CAG-hCAS9-neo
FEATURES             Location/Qualifiers
     source          1..13335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3..20)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3..19)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    29..152
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             652..843
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(837..859)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    945..1085
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1000..1019
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1100..1117)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1271..1859)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1351..1370)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2030..2821,2822..2890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2653..2672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2891..2995)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3063..3081
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    complement(3109..3232)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    3764..4265
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    4342..4366
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        4388..4767
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4770..5045
                     /label=chicken beta-actin promoter
     intron          5046..6054
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5978..6000
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6062..6081
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6122..6187
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6194..6214
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6239..10339
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6239..10339
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             10340..10387
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    join(10416..10989,10990..10992,10993..11001)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10583..10600)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10810..10829
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             11002..11805
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11065..11084)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11675..11694
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11837..11854)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11843..12067
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(12112..12136)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    12309..12838
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc
      181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
      361 agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga
      421 tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc
      481 tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt
      541 tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc
      601 atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa
      661 caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg
      721 gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac
      781 cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
      841 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      901 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
      961 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     1021 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     1081 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1141 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1201 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1261 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1321 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1381 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1441 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1621 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1681 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     1741 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1801 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1861 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1921 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1981 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2041 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2101 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2161 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2221 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2281 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2341 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2401 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2461 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2521 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2581 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2641 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2701 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2761 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2821 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2881 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2941 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3001 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3061 aaaataggcg tatcacgagg ccctttcgtc ttcaagaaaa ttgtcgacca tagtgactgg
     3121 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3181 ttgatattta tatcatttta cgtttctcgt tcaactttat tatacaaagt tgcggacgcg
     3241 atcgcgaccg gcggataaca atttcacaca ggattaccat ccctccctcg acttcccctc
     3301 ttccgatgtt gagcccctcc agccggtcct ggactttgtc tccttccctg ccctgccctc
     3361 tcctgaacct gagccagctc ccatagctca gtctggtcta tctgcctggc cctggccatt
     3421 gtcactttgc gctgccctcc tctcgccccc gagtgccctt gctgtgccgc cggaactctg
     3481 ccctctaacg ctgccgtctc tctcctgagt ccggaccact ttgagctcta ctggcttctg
     3541 cgccgcctct ggcccactgt ttccccttcc caggcaggtc ctgctttctc tgacctgcat
     3601 tctctcccct gggcctgtgc cgctttctgt ctgcagcttg tggcctgggt cacctctacg
     3661 gctggcccag atccttccct gccgcctcct tcaggttccg tcttcctcca ctccctcttc
     3721 cccttgctct ctgctgtgtt gctgcccaag gatgctcttt ccggagcact tccttctcgg
     3781 cgctgcacca cgtgatgtcc tctgagcgga tcctccccgt gtctgggtcc tctccgggca
     3841 tctctcctcc ctcacccaac cccatgccgt cttcactcgc tgggttccct tttccttctc
     3901 cttctggggc ctgtgccatc tctcgtttct taggatggcc ttctccgacg gatgtctccc
     3961 ttgcgtcccg cctccccttc ttgtaggcct gcatcatcac cgtttttctg gacaacccca
     4021 aagtaccccg tctccctggc tttagccacc tctccatcct cttgctttct ttgcctggac
     4081 accccgttct cctgtggatt cgggtcacct ctcactcctt tcatttgggc agctccccta
     4141 ccccccttac ctctctagtc tgtgctagct cttccagccc cctgtcatgg catcttccag
     4201 gggtccgaga gctcagctag tcttcttcct ccaacccggg cccctatgtc cacttcagga
     4261 cagcatgttt gctgcctcca gggatcctgt gtccccgagc tgggaccacc ttataaggcg
     4321 cataacgata ccacgatatc aacaagtttg tacaaaaaag caggctggcg ccggaaccaa
     4381 ttcagtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     4441 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4501 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4561 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
     4621 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4681 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4741 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
     4801 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
     4861 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
     4921 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     4981 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
     5041 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
     5101 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     5161 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
     5221 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
     5281 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
     5341 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     5401 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     5461 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     5521 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg
     5581 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     5641 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc
     5701 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     5761 cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc
     5821 cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     5881 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag
     5941 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     6001 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     6061 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcgt cgaccaccat
     6121 ggactataag gaccacgacg gagactacaa ggatcatgat attgattaca aagacgatga
     6181 cgataagatg gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccga
     6241 caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg ccgtgatcac
     6301 cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag
     6361 catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac
     6421 ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct
     6481 gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga
     6541 agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat
     6601 cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact
     6661 ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat
     6721 caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga
     6781 caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa
     6841 cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct
     6901 ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat
     6961 tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc
     7021 caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat
     7081 cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct
     7141 gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat
     7201 caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca
     7261 gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta
     7321 cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa
     7381 gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca
     7441 gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat
     7501 tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa
     7561 gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt
     7621 cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt
     7681 ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct
     7741 gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa
     7801 cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg
     7861 cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa
     7921 gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg
     7981 cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa
     8041 ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac
     8101 cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct
     8161 gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct
     8221 gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt
     8281 cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct
     8341 gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga
     8401 gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa
     8461 ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga
     8521 aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa
     8581 gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga
     8641 aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat
     8701 gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt
     8761 gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa
     8821 gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta
     8881 ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa
     8941 ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt
     9001 ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa
     9061 gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct
     9121 ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca
     9181 ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc
     9241 taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat
     9301 cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat
     9361 catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct
     9421 gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac
     9481 cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac
     9541 aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag
     9601 aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc
     9661 tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga
     9721 gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct
     9781 ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc
     9841 cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa
     9901 gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta
     9961 tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca
    10021 caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct
    10081 ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat
    10141 cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc
    10201 cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt
    10261 gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct
    10321 gtctcagctg ggaggcgaca aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa
    10381 gaaaaagtaa gaattctagc ggccgccgcc ccccccccct ctccctcccc cccccctaac
    10441 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
    10501 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
    10561 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
    10621 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
    10681 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
    10741 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
    10801 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
    10861 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
    10921 aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
    10981 gatgataata tggccacaac catgggatcg gccattgaac aagatggatt gcacgcaggt
    11041 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
    11101 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
    11161 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
    11221 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
    11281 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
    11341 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
    11401 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
    11461 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
    11521 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
    11581 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
    11641 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
    11701 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
    11761 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgacgtac gcgatgatct
    11821 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
    11881 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
    11941 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
    12001 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
    12061 tctatggctt ctgaggcgga aagaacctgc aactagtctc gagatatcta gacccagctt
    12121 tcttgtacaa agtggttgat atctctatag tcgcagtagg cggtccttag gcctcctcct
    12181 tcctagtctc ctgatattgg gtctaacccc cacctcctgt taggcagatt ccttatctgg
    12241 tgacacaccc ccatttcctg gagccatctc tctccttgcc agaacctcta aggtttgctt
    12301 acgatggagc cagagaggat cctgggaggg agagcttggc agggggtggg agggaagggg
    12361 gggatgcgtg acctgcccgg ttctcagtgg ccaccctgcg ctaccctctc ccagaacctg
    12421 agctgctctg acgcggccgt ctggtgcgtt tcactgatcc tggtgctgca gcttccttac
    12481 acttcccaag aggagaagca gtttggaaaa acaaaatcag aataagttgg tcctgagttc
    12541 taactttggc tcttcacctt tctagtcccc aatttatatt gttcctccgt gcgtcagttt
    12601 tacctgtgag ataaggccag tagccagccc cgtcctggca gggctgtggt gaggaggggg
    12661 gtgtccgtgt ggaaaactcc ctttgtgaga atggtgcgtc ctaggtgttc accaggtcgt
    12721 ggccgcctct actccctttc tctttctcca tccttctttc cttaaagagt ccccagtgct
    12781 atctgggaca tattcctccg cccagagcag ggtcccgctt ccctaaggcc ctgctctggg
    12841 cttctgggtt tgagtccttg gcaagcccag gagaggcgct caggcttccc tgtccccctt
    12901 cctcgtccac catctcatgc ccctggctct cctgcccctt ccctacaggg gttcctggct
    12961 ctgctcttca gactgagccc cgttcccctg catccccgtt cccctgcatc ccccttcccc
    13021 tgcatccccc agaggcccca ggccacctac ttggcctgga ccccacgaga ggccacccca
    13081 gccctgtcta ccaggctgcc ttttgggtgg attctcctcc aactgtgggg tgactgcttg
    13141 gcaaactcac tcttcggggt atcccaggag gcctggagca ttggggtggg ctggggttca
    13201 gagaggaggg attcccttct caggttacgt ggccaagaag caggggagct gggtttgggt
    13261 caggtctggg tgtggggtga ccagcttatg ctgtttgccc aggacagcct agttttagca
    13321 ctgaaaccct cagtc
//
