LOCUS       sequence-322361-       10453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of hyeA3A-BE4max.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Zhang X, Chen L, Zhu B, Wang L, Chen C, Hong M, Huang Y, Li H, Han 
            H, Cai B, Yu W, Yin S, Yang L, Yang Z, Liu M, Zhang Y, Mao Z, Wu Y, 
            Liu M, Li D
  TITLE     Increasing the efficiency and targeting range of cytidine base 
            editors through fusion of a single-stranded DNA-binding protein 
            domain.
  JOURNAL   Nat Cell Biol. 2020 Jun;22(6):740-750. doi: 
            10.1038/s41556-020-0518-8. Epub 2020 May 11.
  PUBMED    32393889
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-020-0518-8"; journalName: "Nat Cell Biol"; date: 
            "2020-06"; volume: "22"; issue: "6"; pages: "740-750"
COMMENT     Sequence Label: hyeA3A-BE4max
FEATURES             Location/Qualifiers
     source          1..10453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(109..128)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        320..568
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     320..340
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     491..510
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        587..662
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        682..1061
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1062..1265
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1216..1236
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1347..1367
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2496..2519,2520..2522,2523..6596)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2496..6596
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6627..6875
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6906..7154
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7197..7217
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7227..7283
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7284..7286,7287..7289,7290..8000)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7284..7286,7287..7289,7290..8000)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7590..7609)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7937..7958
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8013..8033
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(8056..8073)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8062..8286
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8370..8392)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8381..8397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8405..8411,8412..8429,8430..8435))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8450..8471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8588..8605)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8759..9347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8839..8858)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9518..10309,10310..10378))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10141..10160
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10379..10453,1..30))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
       61 cgacggatcg ggagatcgat ctcccgatcc cctagggtcg actctcagta caatctgctc
      121 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
      181 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
      241 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgtgg
      301 atccctgcag ttaattaaag agggcctatt tcccatgatt ccttcatatt tgcatatacg
      361 atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa agatattagt
      421 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
      481 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
      541 atatatcttg tggaaaggac gaaacaccgg gtcttcgaga agacctgttt tagagctaga
      601 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      661 gcttttttta tgcatggtac cgacattgat tattgactag ttattaatag taatcaatta
      721 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      781 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      841 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      901 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      961 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     1021 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     1081 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1141 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     1201 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1261 gagctggttt agtgaaccgt cagatccgct agagatccgc ggccgccacc atgaagagga
     1321 ccgccgatgg ctctgagttc gagagcccca agaagaagcg gaaggtggag gcatctccag
     1381 caagcggacc aaggcacctg atggaccccc acatcttcac ctctaacttt aacaatggca
     1441 tcggcaggca caagacatac ctgtgctatg aggtggagcg cctggacaac ggcaccagcg
     1501 tgaagatgga tcagcacaga ggcttcctgc acggccaggc caagaatctg ctgtgcggct
     1561 tctacggccg gcacgcagag ctgagatttc tggacctggt gcctagcctg cagctggatc
     1621 cagcccagat ctatagggtg acctggttca tcagctggtc cccatgcttt tcctggggat
     1681 gtgcaggaga ggtgcgcgcc ttcctgcagg agaatacaca cgtgcggctg agaatctttg
     1741 ccgcccggat ctacgactat gatcctctgt acaaggaggc cctgcagatg ctgagagacg
     1801 caggagccca ggtgtccatc atgacctatg atgagttcaa gcactgctgg gacacatttg
     1861 tggatcacca gggctgtccc tttcagcctt gggacggact ggatgagcac tcccaggccc
     1921 tgtctggcag gctgagggcc atcctgcaga accagggcaa tagcggagga tctagcggag
     1981 gatcaagcgg aagcgagact cctggaacca gcgaaagcgc aaccccagaa agcagcggag
     2041 gaagtagcgg aggaagcatg gcaatgcaga tgcagcttga agcaaatgca gatacttcag
     2101 tggaagaaga aagctttggc ccacaaccca tttcacggtt agagcagtgt ggcataaatg
     2161 ccaacgatgt gaagaaattg gaagaagctg gattccatac tgtggaggct gttgcctatg
     2221 cgccaaagaa ggagctaata aatattaagg gaattagtga agccaaagct gataaaattc
     2281 tggctgaggc agctaaatta gttccaatgg gtttcaccac tgcaactgaa ttccaccaaa
     2341 ggcggtcaga gatcatacag attactactg gctccaaaga gcttgacaaa ctacttcaat
     2401 ctggcggatc tagcggtgga tctagcggct ctgagacccc tggaacatcc gaatccgcca
     2461 ctccagagag cagcggaggc tcttctggag gatcagacaa gaagtacagc atcggcctgg
     2521 ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca
     2581 agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag
     2641 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa
     2701 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga
     2761 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg
     2821 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg
     2881 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg
     2941 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga
     3001 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc
     3061 agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg
     3121 ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc
     3181 ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc
     3241 ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca
     3301 cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt
     3361 ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca
     3421 ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc
     3481 aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga
     3541 ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg
     3601 aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc
     3661 tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca
     3721 tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt
     3781 acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct
     3841 actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg
     3901 aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga
     3961 gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca
     4021 agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg
     4081 tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg
     4141 acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca
     4201 agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct
     4261 ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg
     4321 aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag
     4381 agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc
     4441 agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca
     4501 tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca
     4561 acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga
     4621 aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca
     4681 gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag
     4741 tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca
     4801 cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag
     4861 agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga
     4921 agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca
     4981 tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg
     5041 actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg
     5101 tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca
     5161 agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg
     5221 aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc
     5281 acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga
     5341 tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt
     5401 tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga
     5461 acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt
     5521 acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg
     5581 gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga
     5641 ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg
     5701 gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc
     5761 cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta
     5821 tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga
     5881 agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg
     5941 aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg
     6001 aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag
     6061 tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc
     6121 ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct
     6181 ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg
     6241 aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca
     6301 tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag
     6361 tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca
     6421 tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca
     6481 ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc
     6541 agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga ggtgacagcg
     6601 gcgggagcgg cgggagcggg gggagcacta atctgagcga catcattgag aaggagactg
     6661 ggaaacagct ggtcattcag gagtccatcc tgatgctgcc tgaggaggtg gaggaagtga
     6721 tcggcaacaa gccagagtct gacatcctgg tgcacaccgc ctacgacgag tccacagatg
     6781 agaatgtgat gctgctgacc tctgacgccc ccgagtataa gccttgggcc ctggtcatcc
     6841 aggattctaa cggcgagaat aagatcaaga tgctgagcgg aggatccgga ggatctggag
     6901 gcagcaccaa cctgtctgac atcatcgaga aggagacagg caagcagctg gtcatccagg
     6961 agagcatcct gatgctgccc gaagaagtcg aagaagtgat cggaaacaag cctgagagcg
     7021 atatcctggt ccataccgcc tacgacgaga gtaccgacga aaatgtgatg ctgctgacat
     7081 ccgacgcccc agagtataag ccctgggctc tggtcatcca ggattccaac ggagagaaca
     7141 aaatcaaaat gctgtctggc ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca
     7201 agaagaagag gaaagtcgga agcggagcta ctaacttcag cctgctgaag caggctggag
     7261 acgtggagga gaaccctgga cctatggtga gcaagggcga ggagctgttc accggggtgg
     7321 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     7381 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     7441 agctgcccgt gccctggccc accctcgtga ccaccctgac ctatggagtg cagtgcttca
     7501 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     7561 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     7621 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     7681 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     7741 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     7801 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     7861 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     7921 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     7981 gcatggacga gctgtacaag tctggtggtt ctcccaagaa gaaaaggaaa gtctaagttt
     8041 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     8101 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     8161 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     8221 aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggat gcggtgggct
     8281 ctatggcttc tgaggcggaa agaaccagct ggggctcgat accgtcgacc tctagctaga
     8341 gcttggcgta atcatggtca tagatgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8401 cacacaacat acgagccgga agcataaagt gtaaagccta gggtgcctaa tgagtgagct
     8461 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8521 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8581 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8641 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8701 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8761 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8821 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8881 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8941 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9001 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9061 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9121 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9181 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9241 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9301 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9361 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9421 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9481 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9541 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9601 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9661 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9721 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9781 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9841 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9901 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9961 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10021 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10081 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10141 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10201 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10261 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10321 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10381 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10441 tatttgaatg tat
//
