LOCUS       sequence-322367-        7860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the RADAR defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 7860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG014 (RADAR)
FEATURES             Location/Qualifiers
     source          1..7860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5710..5757
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5977..6636)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6551..6570
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6637..6739)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(7131..7148)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7265..7810)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7345..7364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg
       61 ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
      121 ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcagagga
      181 tttatgcaca aaatcctgat gcgaaatgtt ttcaaaaatt gtcaggttaa cgttcctgca
      241 gatctttgcg ttacatgtca tttctggatc ctttcccgac aggttaggtt gtgattgata
      301 tgatgcccat ctctcatttt agtgatcgtt atccctttat aaacaggagt ttatatgtta
      361 tctatatgca atagacttaa atcgatatac gtgcgcagct tacgattcac ctctctactt
      421 actatttaag gaaaagagtg aggggagaat tgattttcat taagatatta tgagagaatt
      481 atgactagtg aaatagtgtt aaatcttgat ttcccagaat ataaggatga tttttgtact
      541 gatagcattg atgagcaaga taatgagttg tggcagcaac aggccaataa aaagctactt
      601 tcgtttctcg aggtgatggg ggaggaagca agacgatata aagaaaataa ttcccgtagt
      661 acgcatccac attataagac attgagtagt tatcaccatg caatctttat cagtggcgcg
      721 cggggggcgg ggaaaactgt tttcatgaga aatgccagat ttagctggca aaaacattat
      781 aataaagatc taaaacgccc taagctatat tttattgatg tgattgaccc gacgctattg
      841 aatattgatg accgtttttc tgaagtcatt atcgcttcaa tatatgctac ggtagaaaag
      901 cggatgaagc aacctgatat tgcgcagaat atcaaagata attttattaa ttcgcttaag
      961 acgttgtccg gtgcattagg taaatcaaaa gattatgatg aatatagggg cattgatcgt
     1021 attcaaaaat atcgttctgg aatccacctt gaaaaatatt tccatcagtt cttgatttca
     1081 agcgttgagt tactggattg cgatgcgctg gttttgccga ttgatgatgt tgatatgaaa
     1141 atagataacg cttttggtgt tctggacgat attcgctgcc tgttgtcatg tccattagtt
     1201 ctaccattag ttagtgggga taatgatctt tatcggttca ttgccaaaag taaatttgag
     1261 gaattattaa atcgtaaagc aaactctaat tatgctaaag aaggcagcga gatagcagaa
     1321 agattatcag aagcatatat tactaaagta ttccccagcc atgtgaagat acccctccaa
     1381 ccgatagatg agttgttgcc atatctttat atacattcta atgaagatga aaataaacaa
     1441 catacaagct attctgaatt tatcaaactt gtacaacaaa aattctactt tctttgtaat
     1501 gggcaagaac gaagcacaaa ttggccgcag ccgagaagcg cacgtgaagt tacgcaacta
     1561 atccgttctt tacctccgtc tactcttagt aaggaagatg attcgggaac tgatttatgg
     1621 caacgcttcg ctgtctgggc ggaagaacgt cgcgatggat tagcattaac caatgttgaa
     1681 tcttatctgt ttattaagaa tgcgaaagca gtagaagatt taaatctgtc aaatcttatt
     1741 gcttttaatc ctttactgca aaaaggaaaa tatccctggg cagaaaagga tttttataaa
     1801 cagcagtccc aacgtcggaa agagctcaat gcccccgaaa caaattcagg tatccttaat
     1861 accgtatttt ccgaacaaag gaaagatttt attttaagaa gtatgcctgc gctggaactc
     1921 attatggagc ctatgtatgt cactaagacg gtagcagaaa aaaatgataa ttctgcgctt
     1981 atagcgatct atacccattc tgattattac agccagcagc agaacagacg atgtcatata
     2041 ttttttggca gagcttttga aataatgttc tggtcagtat tagcgaaaac tgaaaatctt
     2101 ccacaagaat tttatgaaaa agataagttt aaatctttat ttggtaatat tttcaaaaaa
     2161 gtaccattct actcaatatt ttcaatgaac cctacaaagg ttgttgatga agaaaatgac
     2221 gatggcagtg aacctgattt ttcgcaaaaa ctggacgata gcattaatga actggtggaa
     2281 gatatatata tctgggcaac cagtaataaa ttgcgagcct tcaaaaataa aaatttaata
     2341 cccttaatga cgtgcgtttt taataaggta ttttcacaga tcaatgtact gagaaaaaac
     2401 gtgcaggaca gagttaaatt tagagatgaa catttgtcag atctggctaa gcgatttgag
     2461 tatatgttta ttaatgctat ctttactttc atcagagaag gggtagttgt caataccaat
     2521 gtggcaacag gcgcagctcc tgccagagta cgtaatttat cagagtttaa taggtatgat
     2581 aaaacattat ccaggaatat gtccgggatt ttatccgtga aagaggataa tggcttaacg
     2641 atagtcaaag agagtgaggg cgatatcgca gatctgttat ttgaaatttg gcatagccca
     2701 ttatttaaat taacaaccag gacatgttac ccaataggta aaataaattc gcaaaatacg
     2761 gcccaggaaa atttatcatc agattttaat tcattttttg aaaatggtat caacttcgaa
     2821 ttgataaaac aatattattg gcaaacttca aatcatgata atatcaggac agcagacgtt
     2881 agggaatggg caacttcacg tcttaatgaa gcaatcatcc ttttttcatg gatgaaagaa
     2941 agcaagtcta ttaaagcgaa aattgacgga cagagctacg agggtcggct ctttcgcggg
     3001 cttcagcagg cgctggaagg ttatgaggag gtctgagtat gtttaatcag gatccttatt
     3061 ggctcattcc taccctttgt ctggcatcag accgaatttt ttatgcacaa ttgcgagacc
     3121 acttaggcca gaaaagtagc ggtgaacgca aaaaagaaaa aaatggatat atactggtac
     3181 aggcggcaca agactatcaa ttctattttg gcggccgtat tcggaaagag gatgtgcaaa
     3241 ataatgcctt aatgtggcag atagaaactg gtaatgaaaa ttgcttatcg atgcttgata
     3301 gtttgtcagc atatttcctc acatggcgcg gcaattgttt tgaggtcagg cgtgagcgac
     3361 ttgaaccctg gctgatgatc tgttccgtga tagatcccgc atggattatt gcctatgcat
     3421 accaacaatt gattaaacaa aatgttgtat gtgatagtga gcttatttct ttgctgacag
     3481 aacatcaatg tccatttgcc tttccaaaag gcagagggga catttccttt gctgataatc
     3541 atgtccatct taatggtcat ggttatagtt caatttcaat gctgaacttt atagatggaa
     3601 attataaggt taaaaaaggg ataaaatggc cctatcggca ggaatacacc ctctttgaaa
     3661 gtggtcttct ggataaaaat gatcttcccc gctggctgtc cgcttatagc tcttgcttac
     3721 ttaaaaatgt atataattca tttcaacaag gaaaaagatc cgaggtagat ttcacatgtc
     3781 tgaaggatgc ggtcgaaacg gtgcttgcgg atgaggataa atattatttt ttagaggtag
     3841 cttcgctata tgatgttgtc accttgcagc aaagagtgct ttatgaagcc gcccagcaga
     3901 aatatcactc acatcaacgt tggttactgt atacttgcgg aataatgtta ggtacagaat
     3961 ctgaagatta tgcgaatgcg ctggctaacc tgatccgaat cagcaatatt ctaagaaact
     4021 atatggttgt atctgcggtt ggattgggac aatttattga ttttttcggc ttcaactatc
     4081 gtcgaataac aaagccagct gatacaaaca accgagttca ttatgattct tctgctggta
     4141 tttccagaga atatcgtgtc tctcctgatt ttgtactggg tagcggcgta atgcctgata
     4201 tatatgccag gcaacttttc gatttttatt gtacccaagc acgcaagggc gtacccgaac
     4261 aaggacatat tgttgttcat tttacacgtt cctttcctga caaaaaatca acatatgata
     4321 aattgctaac cgagtgtcgc gaacggttac gttctcagtg tgattatttt ggccgttttt
     4381 taacatcgct tactttgcag tcgatagaat ataaaaattt atctactgat gaagatcgaa
     4441 gcatagacat tagaaaatta gttcgtggct atgatgttgc tggaaatgaa aacgagctac
     4501 aaatagaggt atttgccccg gttctccggg tactgcgtgc tgctaaattt aaaggggagg
     4561 gggtgaactt taaaaggcta cagcgccctt ttattactgt acatgctggt gaggattatt
     4621 gtcatatact cagtggcctt cgggctatgg atgaagccgt tgaattttgt atgttaggag
     4681 aaggcgatcg tatagggcat ggattagctc tgggagtaga tataaaacta tgggcgaatc
     4741 gccaaaagcg agcatacctg acggttggac aacatcttga taatttggtt tgggcatatc
     4801 atcaggcagt attactttct caacatattg tcgagcatat accagtaatg catgaattaa
     4861 gggataagat ccattattgg tctcatcaat tatatagtga aacttatacg ccagatttac
     4921 tctttaaagc atggctgctc cgccgtaact ggccggatta taagtcaatc atatctgatc
     4981 cagcaaatat caatgaatgg gtgcctgacc aacatatttt agtcagtaca gatgagacta
     5041 cagctaaggc cagaaaaatt tgggaacgtt atttaaatag cggtctggca gaaaatgatg
     5101 tttttaacag aataatttca gtaaattgtg cgcccgatac agcgcaaaat ttttcaatga
     5161 cctttaatga aaatgaagat attttatcca aaggggaatt attattgtat gaagctatcc
     5221 aggatttctt aatcgaaaaa tatagtaggt tgggtttagt catagaagct tgtccaacct
     5281 caaatattta tattggcaga ctggagaaat atcatgagca cccattattc cgttggaatc
     5341 ctcctgactc ccaatggatt aaacctggtg ggaaatttaa tcgctttgga ttgcgcacag
     5401 gacctttatc tgtctgtata aatacagatg acagtgcatt gatgccaacc acaattgaaa
     5461 acgaacatcg cttaatgaga gactgcgcca tacattttta tggtattgga acatggatgg
     5521 cggatttatg gataaactca atacgcataa aaggtattga aatattcaaa ggtaatcatt
     5581 taagtcagga tttagataat ttaatctaaa tgtaaacaag aaatccacgc aaatgcgtgg
     5641 attttaagtc aacttattat tctctgaaac ggtttaaccg ttcggaacaa cagattaaat
     5701 ccgcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     5761 aaacctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta
     5821 aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg
     5881 aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcacc
     5941 aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc
     6001 gcaatactgt tgtaattcat ttaacattct gccgacatgg aagccatcac agacggcatg
     6061 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     6121 agtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     6181 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     6241 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     6301 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     6361 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcatcg ccatacggaa
     6421 ctctggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     6481 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     6541 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     6601 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     6661 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     6721 acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt
     6781 atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta
     6841 ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt
     6901 tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg
     6961 gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctagcggagt gtatactggc
     7021 ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag
     7081 gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact
     7141 gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga
     7201 gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc
     7261 cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt
     7321 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg
     7381 tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt
     7441 tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt
     7501 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7561 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
     7621 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
     7681 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
     7741 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
     7801 acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt
//
