LOCUS       sequence-322369-       11340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  virus packaged, doxycyline inducible expression of Cyclin E1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Matson JP, Dumitru R, Coryell P, Baxley RM, Chen W, Twaroski K, 
            Webber BR, Tolar J, Bielinsky AK, Purvis JE, Cook JG
  TITLE     Rapid DNA replication origin licensing protects stem cell 
            pluripotency.
  JOURNAL   Elife. 2017 Nov 17;6. doi: 10.7554/eLife.30473.
  PUBMED    29148972
REFERENCE   2  (bases 1 to 11340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-11-17"; volume: "6. doi"; pages: " 10.7554/eLife.30473"
COMMENT     Sequence Label: pInducer20 Cyclin E1
FEATURES             Location/Qualifiers
     source          1..11340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        180..1391
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1228..1247
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(1450..2067,2068..2073,2074..2109,2110..2112,2113..2148,
                     2149..2151,2152..2187,2188..2196)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    2345..2807
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2401..2418)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2628..2647
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2823..3617
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2823..3617
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2877..2896)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3487..3506
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3794..4382
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4265..4276)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4457..4690
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4809..4827)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4895..4999
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5000..5068,5069..5860)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6035..6623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6524..6543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6777..6794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7034..7055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7070..7075,7076..7093,7094..7100)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7108..7124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7113..7135
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7132..7148
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7132..7148
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7198..7217
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7409..8042
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8089..8214
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8709..8942
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9127..9171
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9464..9581
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9643..9661
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9685..9703
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9727..9745
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9769..9787
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9811..9829
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9853..9871
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9895..9913
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        9946..9984
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9981..10005
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    10114..10138
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(11330..11340,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
       61 actatcagtc aaaataaaat ymccagacta cgcttagtaa tgattaatta aactagaaat
      121 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agtaacccac ccaagatctg
      181 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc
      241 cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc
      301 ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg acattttagg
      361 acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga
      421 aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg
      481 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc
      541 gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg
      601 gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc
      661 aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc
      721 agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag
      781 gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc
      841 gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg
      901 acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt
      961 atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg
     1021 tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc
     1081 ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc
     1141 gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg
     1201 ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt
     1261 tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa
     1321 tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt
     1381 ttttgttaga cgaagcttgg taccgagctc ggatctccac cccgtaccgg tcctgcagtc
     1441 gaattcacca tgtctagact ggacaagagc aaagtcataa acggagctct ggaattactc
     1501 aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag
     1561 cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc
     1621 gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc atggcaagac
     1681 tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg cgacggggct
     1741 aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc
     1801 gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg
     1861 ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa
     1921 agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat tgagctgttc
     1981 gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg
     2041 gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga ttttgactta
     2101 gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc tgctgacgct
     2161 cttgacgatt ttgaccttga catgctcccc gggtaactaa gtaaggatcg atccaagata
     2221 tcgtattctt aactatgttg ctccttttac gctatgtgga tacksksckw wgccgcttgg
     2281 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     2341 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     2401 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     2461 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     2521 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     2581 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     2641 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     2701 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     2761 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct taccggtcca
     2821 ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     2881 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     2941 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     3001 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     3061 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     3121 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     3181 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     3241 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     3301 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     3361 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     3421 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     3481 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     3541 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     3601 ttgacgagtt cttctgatgt acaagtaaag cggccgcgac tctagatcat aatcagccat
     3661 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     3721 aaacataaaa tgaatgcaat tgttgttgtt tagtccctcc caattcgata tcaagcttat
     3781 cgcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3841 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3901 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     3961 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4021 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4081 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4141 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaary yrwcgtcctt
     4201 tccwtggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4261 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4321 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4381 gcctgagrtc ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     4441 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     4501 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4561 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4621 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4681 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     4741 tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag
     4801 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4861 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4921 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4981 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5041 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5101 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5161 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5221 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5281 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5341 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5401 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5461 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5521 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5581 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5641 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5701 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5761 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5821 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5881 atatatactt tagattgatt taaaacttca ttywywrkyw rarmrkwwck mkgacttgac
     5941 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     6001 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6061 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6781 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6841 ggccgattca ttaatgcagc tggcwcrwsr sngwytmmyk wttkkwawky rkgcagaggc
     6901 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6961 aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg
     7021 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7081 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7141 gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7201 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     7261 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
     7321 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     7381 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     7441 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     7501 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     7561 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     7621 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     7681 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     7741 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     7801 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     7861 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7921 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7981 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8041 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     8101 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8161 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8221 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8281 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8341 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8401 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8461 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8521 caaaacaaaa gtaagaccac cgcacagcaa gcggccgnnc tgatcttcag acctggagga
     8581 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     8641 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     8701 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     8761 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     8821 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     8881 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     8941 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     9001 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     9061 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     9121 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     9181 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     9241 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     9301 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     9361 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     9421 cgacggtatc gccgaattca caaatggcag tattcatcca caattttaaa agaaaagggg
     9481 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     9541 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     9601 gcagagatcc astttggmck mgratcswtw wsynnnnnnn actccctatc agtgatagag
     9661 aamnnngwaw gtcgagttta cnnntcccta tcagtgatag agaamrrtgn nnntcgagtt
     9721 tacnnntccc tatcagtgat agagaamnnn gwawgtcgag tttacnnntc cctatcagtg
     9781 atagagaamn nngwawgtcg agtttacnnc tccctatcag tgatagagaa magtgwawgt
     9841 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     9901 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg
     9961 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
    10021 ccacgctgtt ttgacctcca tagaagacac cgggacygay ysatagtgac ctgttcgttg
    10081 caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa agcaggctac
    10141 catgaaggag gacggcggcg cggagttctc ggctcgctcc aggaagagga aggcaaacgt
    10201 gaccgttttt ttgcaggatc cagatgaaga aatggccaaa atcgacagga cggcgaggga
    10261 ccagtgtggg agccagcctt gggacaataa tgcagtctgt gcagacccct gctccctgat
    10321 ccccacacct gacaaagaag atgatgaccg ggtttaccca aactcaacgt gcaagcctcg
    10381 gattattgca ccatccagag gctccccgct gcctgtactg agctgggcaa atagagagga
    10441 agtctggaaa atcatgttaa acaaggaaaa gacatactta agggatcagc actttcttga
    10501 gcaacaccct cttctgcagc caaaaatgcg agcaattctt ctggattggt taatggaggt
    10561 gtgtgaagtc tataaacttc acagggagac cttttacttg gcacaagatt tctttgaccg
    10621 gtatatggcg acacaagaaa atgttgtaaa aactctttta cagcttattg ggatttcatc
    10681 tttatttatt gcagccaaac ttgaggaaat ctatcctcca aagttgcacc agtttgcgta
    10741 tgtgacagat ggagcttgtt caggagatga aattctcacc atggaattaa tgattatgaa
    10801 ggcccttaag tggcgtttaa gtcccctgac tattgtgtcc tggctgaatg tatacatgca
    10861 ggttgcatat ctaaatgact tacatgaagt gctactgccg cagtatcccc agcaaatctt
    10921 tatacagatt gcagagctgt tggatctctg tgtcctggat gttgactgcc ttgaatttcc
    10981 ttatggtata cttgctgctt cggccttgta tcatttctcg tcatctgaat tgatgcaaaa
    11041 ggtttcaggg tatcagtggt gcgacataga gaactgtgtc aagtggatgg ttccatttgc
    11101 catggttata agggagacgg ggagctcaaa actgaagcac ttcaggggcg tcgctgatga
    11161 agatgcacac aacatacaga cccacagaga cagcttggat ttgctggaca aagcccgagc
    11221 aaagaaagcc atgttgtctg aacaaaatag ggcttctcct ctccccagtg ggctcctcac
    11281 cccgccacag agcggtaaga agcagagcag cgggccggaa atggcgtgaa cccagctttc
//
