LOCUS       sequence-322390-       12572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express N-terminal ISC mutant POLE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12572)
  AUTHORS   Sviderskiy VO, Blumenberg L, Gorodetsky E, Karakousi TR, Hirsh N, 
            Alvarez SW, Terzi EM, Kaparos E, Whiten GC, Ssebyala S, Tonzi P, Mir
            H, Neel BG, Huang TT, Adams S, Ruggles KV, Possemato R
  TITLE     Hyperactive CDK2 Activity in Basal-like Breast Cancer Imposes a 
            Genome Integrity Liability that Can Be Exploited by Targeting DNA 
            Polymerase epsilon.
  JOURNAL   Mol Cell. 2020 Oct 28. pii: S1097-2765(20)30723-1. doi: 
            10.1016/j.molcel.2020.10.016.
  PUBMED    33152268
REFERENCE   2  (bases 1 to 12572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2020 Oct 28. pii: S1097-2765(20)30723-1. doi: 
            10.1016/j.molcel.2020.10.016."
COMMENT     Sequence Label: pMXS-IRES-Blast POLE1 N-ISCmut
FEATURES             Location/Qualifiers
     source          1..12572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    117..474
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             533..949
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     879..901
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     917..933
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    959..1333
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1271..1287
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1382..1447
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8386..8938
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8530..8547)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8757..8776
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8950..9345
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             9516..9985
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(10115..10132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10286..10874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10366..10385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11038..11829,11830..11898))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11661..11680
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11899..12003)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             join(12033..12572,1..54)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
       61 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      121 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
      181 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      241 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
      301 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg caccccccta
      361 ataggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
      421 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
      481 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac
      541 tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca
      601 caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc
      661 aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt
      721 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
      781 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
      841 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
      901 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc
      961 atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac
     1021 aagagttact aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca
     1081 cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc
     1141 tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga
     1201 acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga
     1261 cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc
     1321 atcctctaga ctgccggatc tagctagtta attaaggatc cctaaatcgc aattgaccat
     1381 ggactacaag gaccacgacg gtgactacaa ggaccacgac atcgactaca aggacgacga
     1441 cgacaaggga ggaggcggag gtggaatgtc tctgaggagc ggcgggcggc ggcgcgcgga
     1501 cccaggcgcg gatggcgagg ccagcaggga tgatggcgcc acttcctcag tttcggcact
     1561 caagcgcctg gaacggagtc agtggacgga taagatggat ttgcggtttg gttttgagcg
     1621 gctgaaggag cctggtgaga agacaggctg gctcattaac atgcatccta ccgagatttt
     1681 agatgaagat aagcgcttag gcagtgcggt agactattat ttcatccaag atgacggaag
     1741 cagatttaag gtggctttgc cctataaacc gtatttctac attgcgacca gaaagggttg
     1801 tgagcgagaa gtttcatctt ttctctccaa gaagtttcag ggcaaaattg caaaagtgga
     1861 gactgtcccc aaagaggatc tggacttgcc aaatcacttg gtgggtttga agcgaaatta
     1921 catcaggctg tccttccaca ctgtggagga tcttgtcaaa gtgaggaagg agatctcccc
     1981 tgccgtgaag aagaacaggg agcaggatca cgccagcgac gcgtacacag ctctgctttc
     2041 cagtgttctg cagaggggcg gtgtcattac tgatgaagag gaaacctcta agaagatagc
     2101 cgatcaacta gataatatcg tggacatgcg cgagtacgat gttccctacc acatccgcct
     2161 ctccattgac ctgaagatcc acgtggctca ttggtacaat gtcagatacc gaggaaatgc
     2221 ttttccggta gaaatcaccc gccgagatga ccttgttgaa cgacctgacc ctgtggtttt
     2281 ggcatttgac attgagacga ccaaactgcc cctcaagttt cctgatgctg agacagacca
     2341 gattatgatg atttcctaca tgatcgatgg ccagggctac ctcatcacca acagggagat
     2401 tgtttcagaa gatattgaag attttgagtt cacccccaag ccagaatatg aaggcccctt
     2461 ttgtgtcttc aatgaacccg atgaggctca tctgatccaa aggtggtttg aacacgtcca
     2521 ggagaccaaa cccaccatca tggtcaccta caacggggac ttttttgact ggccatttgt
     2581 ggaggcccgg gcagcagtcc acggtctgag catgcagcag gagataggct tccagaagga
     2641 cagccagggg gagtacaagg cgccccagtg catccacatg gactgcctca ggtgggtgaa
     2701 gagggacagt taccttcctg tgggcagtca taatctcaag gcggccgcca aggccaagct
     2761 aggctatgat cccgtggagc tagacccgga ggacatgtgc cggatggcca cggagcagcc
     2821 ccagactctg gccacgtatt ctgtgtcaga tgctgtcgcc acttactacc tgtacatgaa
     2881 gtacgtccac ccattcatct ttgctctgtg caccattatt cccatggagc ccgacgaggt
     2941 gctgcggaag ggctctggca ctctgtgtga ggccttgctg atggtgcagg ccttccacgc
     3001 caacatcatc ttccccaaca agcaagagca ggagttcaat aagctgacgg acgacggaca
     3061 cgtgctggac tctgagacct acgtcggggg ccacgtggag gccctcgagt ctggggtttt
     3121 ccgcagcgat atcccttgcc ggtttaggat gaatcctgcc gcctttgact tcctgctgca
     3181 gcgggttgag aagaccttgc gccacgccct tgaggaagag gagaaagtgc ctgtggagca
     3241 agtcaccaac tttgaagagg tgtgtgatga gattaagagc aagcttgcct ccctgaagga
     3301 cgttcccagc cgcatcgagt gtccactcat ctaccacctg gacgtggggg ccatgtaccc
     3361 caacatcatc ctgaccaacc gcctgcagcc ctctgccatg gtggacgaag ccaccagtgc
     3421 tgccagtgac ttcaataagc ctggagcaaa cagccagcgg aagatggcct ggcagtggag
     3481 gggcgagttc atgccagcca gtcgcagcga ataccatcgg atccagcacc agctggagtc
     3541 agagaagttc ccccccttgt tcccagaggg gccagctcgg gcctttcatg aactgtcccg
     3601 cgaggaacag gcgaaatacg agaagagaag gctggcggat tactgccgga aagcctacaa
     3661 gaagatccac atcaccaagg tggaagagcg tctcaccacc atctgccagc gggaaaactc
     3721 cttctacgtg gacaccgtgc gtgccttccg ggacaggcgt tacgagttca aagggctcca
     3781 caaggtgtgg aaaaagaagc tctcggcggc cgtggaggtg ggcgacgcgg ctgaggtgaa
     3841 gcgctgcaag aacatggagg tgctgtatga ctcgctgcag ctggcccaca agtgcatcct
     3901 gaactccttc tatggctatg tcatgcgcaa gggggctcgc tggtactcca tggagatggc
     3961 tggcatcgtc tgcttcacag gggccaacat catcacccag gcacgggagc tgatcgagca
     4021 gattgggagg cccttagagc tggacacaga tggtatatgg tgcgtcctgc ccaacagctt
     4081 cccagaaaat tttgtcttca agacgaccaa tgtgaagaag cccaaagtga ccatctccta
     4141 cccaggcgcc atgttgaaca tcatggtcaa ggaaggcttc accaatgacc agtaccagga
     4201 gctggctgag ccgtcctcac tcacctacgt cacccgctca gagaacagca tcttttttga
     4261 ggttgatggg ccctaccttg ccatgattct tccagcctcc aaggaagaag gcaagaaatt
     4321 gaagaagagg tatgctgtgt tcaatgaaga cggttctctg gctgagctca agggctttga
     4381 ggtcaaacgc cgcggggaac tgcagctgat taagatcttc caatcctcgg tgtttgaggc
     4441 cttcctcaag ggcagcacgc tggaagaggt gtatggctct gtagccaagg tggctgacta
     4501 ctggctggac gtgctgtaca gcaaggcagc caacatgcct gactctgagc tattcgagct
     4561 catctctgag aaccgttcca tgtctcggaa gctggaagat tacggggagc agaagtctac
     4621 gtccatcagc acagcaaagc gcctggccga gttcctggga gaccagatgg tcaaggatgc
     4681 agggctgagt tgccgctaca tcatctcccg caagcccgag ggctcccctg tcacggagag
     4741 ggccatccca cttgccattt tccaagcaga gcccacggtg aggaagcact ttctccggaa
     4801 atggctcaag agctcttccc ttcaagactt tgatattcga gcaattctgg attgggacta
     4861 ctacattgag cggctgggaa gcgccatcca gaagatcatc accatccctg cggccctgca
     4921 gcaggtaaag aacccagtgc cacgtgtcaa acaccccgac tggctgcaca aaaaactgct
     4981 ggagaagaat gatgtctaca agcagaagaa gatcagtgag ctcttcaccc tggagggcag
     5041 gagacaggtc acgatggccg aggcctcaga agacagtccg aggccaagtg ctcctgacat
     5101 ggaggacttc ggcctcgtaa agctgcctca cccagcagcc cctgtcactg tgaagaggaa
     5161 gcgagttctt tgggagagcc aggaggagtc ccaggacctc acgccgactg tgccctggca
     5221 ggaaatcttg gggcagcctc ccgccctggg aaccagccag gaggaatggc ttgtctggct
     5281 ccggttccac aagaagaagt ggcagctgca ggcccggcag cgcctcgccc gcaggaagag
     5341 gcagcgtctg gagtcggcag agggtgtgct caggcccggg gccatccggg atggtcctgc
     5401 cacggggctg gggagcttct tgcgaagaac tgcccgcagc atcctggacc ttccgtggca
     5461 gattgtgcag atcagcgaga ccagccaggc cggcctgttc aggctgtggg cgctcgttgg
     5521 cagtgacttg cactgcatca ggctgagcat cccccgtgtg ttctacgtga accagcgagt
     5581 cgctaaagcg gaggagggtg cttcgtatcg caaggtaaat cgggtccttc ctcgctccaa
     5641 catggtctac aatctctatg agtattcagt gccagaggac atgtaccagg aacacatcaa
     5701 cgagatcaac gctgagctgt cagcgccaga catcgagggc gtatatgaga ctcaggttcc
     5761 gttactgttc cgggccctgg tgcacctggg ctgtgtgtgt gtggtcaata aacagctggt
     5821 gaggcacctt tcaggctggg aagcagagac ctttgctctt gagcacctgg agatgcgctc
     5881 tctggcccag ttcagctacc tggaaccagg gagtatccgc catatctacc tgtaccacca
     5941 cgcacaggcc cacaaagcgc tcttcgggat cttcatcccc tcacagcgca gggcatccgt
     6001 ctttgtgctg gacactgtgc gcagcaacca gatgcccagc cttggcgccc tgtactcagc
     6061 agagcacggc ctcctcctgg agaaggtggg ccctgagctc ctgccacccc ccaaacacac
     6121 cttcgaagtt cgggcagaaa ctgacctgaa gaccatctgc agagccatcc agcgattcct
     6181 gctcgcctac aaggaggagc gccgggggcc cacactcatc gctgttcagt ccagctggga
     6241 gctgaagagg ctggccagtg aaattcctgt cttggaggaa ttcccactgg tgcctatctg
     6301 tgtggctgac aagatcaact atggggtcct ggactggcag cgccatggag cccggcgcat
     6361 gatccgtcac tacctcaacc tggacacctg cctgtcgcag gccttcgaga tgagcaggta
     6421 ctttcacatt cccattggga acctaccaga ggacatctcc acattcggct ccgacctctt
     6481 ctttgcccgc cacctccagc gccacaacca cctgctctgg ctgtccccta cagcccgccc
     6541 tgacctgggt ggaaaggagg ctgatgacaa ctgtcttgtc atggagttcg atgaccaagc
     6601 cactgttgag atcaacagtt caggctgtta ctccacagtg tgtgtggagc tggaccttca
     6661 gaacctggcc gtcaacacca ttctccagtc tcaccatgtc aacgacatgg agggggccga
     6721 cagcatgggg atcagcttcg acgtgatcca gcaggcctcc ctggaggaca tgatcacggg
     6781 tggtcaggct gccagtgccc cggccagcta cgatgagaca gccctgtgct ctaacacctt
     6841 caggatcctg aagagcatgg tcgtgggctg ggtgaaggag atcacccagt accacaacat
     6901 ctatgcagac aaccaggtga tgcacttcta ccgctggctt cggtcgccat cctctctgct
     6961 tcatgaccct gccctgcacc gcacactcca caacatgatg aagaagctct tcctgcagct
     7021 catcgctgag ttcaagcgcc tggggtcatc agtcatctac gccaacttca accgcatcat
     7081 cctctgtaca aagaagcgcc gtgtggaaga tgccatcgct tacgtggagt acatcaccag
     7141 cagcatccat tcaaaggaga ccttccattc tctgacaatt tctttctctc gatgctggga
     7201 atttcttctc tggatggatc catctaacta tggcggaatc aaaggaaaag tttcatctcg
     7261 tattcactgt ggactgcaag actcccagaa agcaggggga gcagaggatg agcaggaaaa
     7321 tgaggacgat gaggaggaaa gagatgggga ggaggaggaa gaggcggagg aatccaacgt
     7381 ggaggattta ctggaaaaca actggaacat tttgcagttt ttgccacagg cagcctcctg
     7441 ccagaactac ttcctcatga ttgtttcagc gtacatcgtg gccgtgtacc actgcatgaa
     7501 ggacgggctg aggcgcagtg ctccagggag cacccccgtg aggaggaggg gggccagcca
     7561 gctctcccag gaggccgagg gggcggtcgg agcccttccc ggaatgatca ccttctctca
     7621 ggattatgtc gcaaatgagc tcactcagag cttcttcacc atcactcaga agattcagaa
     7681 gaaagtcaca ggctctcgga actccactga gctctcagag atgtttcctg tcctccccgg
     7741 ttcccacttg ctgctcaata accctgccct ggagttcatc aaatacgtgt gcaaggtgct
     7801 gtccctggac accaacatca caaaccaggt gaataagctg aaccgagacc tgcttcgcct
     7861 ggtggatgtc ggcgagttct ccgaggaggc ccagttccga gacccctgcc gctcctacgt
     7921 gcttcctgag gtcatctgcc gcagctgtaa cttctgccgc gacctggacc tgtgtaaaga
     7981 ctcttccttc tcagaggatg gggcggtcct gcctcagtgg ctctgctcca actgtcaggc
     8041 gccctacgac tcctctgcca tcgagatgac gctggtggaa gttctacaga agaagctgat
     8101 ggccttcacc ctgcaggacc tggtctgcct gaagtgccgc ggggtgaagg agaccagcat
     8161 gcctgtgtac tgcagctgcg cgggagactt cgccctcacc atccacaccc aggtcttcat
     8221 ggaacagatc ggaatattcc ggaacattgc ccagcactac ggcatgtcgt acctcctgga
     8281 gaccctggag tggctgctgc agaagaaccc acagctgggc cattaggtcg agctaaatcg
     8341 gtttaaacgc gctaatgcgg ccgctacgta aattccsccc ccctaacgtt actggccgaa
     8401 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     8461 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     8521 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     8581 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     8641 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     8701 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     8761 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     8821 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     8881 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     8941 gccacaacca tggttccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
     9001 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     9061 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     9121 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
     9181 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
     9241 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
     9301 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagtcga cgataaaata
     9361 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     9421 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga
     9481 gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc
     9541 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg
     9601 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     9661 tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
     9721 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
     9781 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
     9841 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct
     9901 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
     9961 actacccgtc agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt
    10021 tcttaagtat ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga
    10081 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    10141 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    10201 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    10261 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    10321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10381 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10441 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10501 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10561 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10621 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10681 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    10741 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10801 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10861 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10921 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10981 ttttgcggcc ggccgcaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    11041 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    11101 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    11161 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    11221 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    11281 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    11341 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    11401 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11461 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11521 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    11581 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    11641 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    11701 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    11761 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    11821 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11881 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11941 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    12001 gcgcacattt ccccgaaaag tgccacctgc ataatgaaag accccacctg taggtttggc
    12061 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagaa
    12121 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct
    12181 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
    12241 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
    12301 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
    12361 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
    12421 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
    12481 cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
    12541 gcagttgcat ccgacttgtg gtctcgctgt tc
//
