LOCUS       sequence-322407-        5584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter for mouse Cxcl1 with Chantico promoter Tbx5 site
            deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Hall JM, Kainth AS, Rowton MJ, Lu E, Haddad G, Keplinger AJ, 
            Scepanovic J , Perez-Cervantes C, Chan SSK, Piccirilli JA, Moskowitz
            IP, Ruthenburg AJ
  TITLE     The cardiac lncRNA Chantico directly regulates Cxcl1 chemokine 
            transcription
  JOURNAL   bioRxiv 2021.12.23.474040
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.12.23.474040"
COMMENT     Sequence Label: pCxcl1-Firefly-Chantico-Tdel
FEATURES             Location/Qualifiers
     source          1..5584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      277..286
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             283..1935
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1979..2100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1990..2009)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2044..2063
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3463..3480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3634..4222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3714..3733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4422..5213,5214..5282))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5387..5435
                     /note="synthetic polyadenylation signal"
     misc_feature    5449..5540
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5489..5508
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ctgaagtctc actactcctc ccccccccaa ccccccagcc gtcacttgtc cagcgaagca
       61 cgtcactccc tcccctttgg gactttgggg caaaaagcaa aaatcccaga gtctaatcct
      121 tgggagtgga gcaaggggga ggagcgatgt cctttccggt tgcagggaaa caccctgtac
      181 tccgggaatt tccctggccc ggagctctgg agtttcgagc ataaaagggg ctcgcgggag
      241 ccctagagct cttggcaatc cggtactgtt ggtaaagcca ccatggaaga tgccaaaaac
      301 attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg cgagcagctg
      361 cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat
      421 atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct
      481 atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg
      541 cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac
      601 gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta
      661 ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata
      721 caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc
      781 ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc
      841 gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag
      901 ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg cgaccccatc
      961 ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt tcaccacggc
     1021 ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt gctcatgtac
     1081 cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca atctgccctg
     1141 ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa gtacgaccta
     1201 agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc
     1261 gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac agaaacaacc
     1321 agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg caaggtggtg
     1381 cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg tgtgaaccag
     1441 cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa caaccccgag
     1501 gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat cgcctactgg
     1561 gacgaggacg agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc
     1621 taccaggtag ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac
     1681 gccggggtcg ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg
     1741 ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt
     1801 acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg
     1861 accggcaagt tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc
     1921 aagatcgccg tgtaataatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     1981 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2041 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2101 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     2161 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccggt cattcagtaa
     2221 atataaatgc agatagccta cgttctgaaa gtggggttct agggtccctt ggttagtttt
     2281 aaaaattcaa agtcactttg agttttgttt tgttttgttt tgttttaaag cttgagagtg
     2341 gccgtgcaga gcaaagttgt ccaattaagt ttatatcagt gacagatggg cttatcagac
     2401 cttcgctctc agacagtagc catggaacac ttagctgctg cctcccgaaa gtgcaagcag
     2461 accagttcag gatctgaagc tttgttttgg tttgacttaa atgtaagtgc aaacagtcgc
     2521 tgtggggtgt gtgtgtgtgt gtgtgtgtga tactggccga tgcaggctga gagtaggggt
     2581 gtctcactcc tgactgcgta ctccccctct gttttacaca ggcttattta ctgttacacg
     2641 gagacttttc ttcgaactgt taaactcttg gctgagtttg tgtggagtat gaggcaaatc
     2701 tctaaccgca agcatgcagt ggaaagatta ggcatacttt tgaaacgagt ttgtgtacca
     2761 gatcatattc ttggctcaag aaagaaaaag aaagggggct ggggaaaggc ttgtggttgc
     2821 tactctggga actaaagaat tcaaaaggct tgttctccat gtttcgtaga cacagacatc
     2881 tggaattgtt gttttaaaat tacaagcaat tgtgtatgct ttgttactgc aacacttatt
     2941 cagaggcaag gacataggtg ccactgttgt cagaaagcca gatgtccttt ccttaaattg
     3001 gcctgcctgg ctgctggcgc ctgcagacac tttgggaatc tcagttagcg gtactgcatg
     3061 gatatagtgc atctgcttct cacacttctg ccaaccttgt gtattggttt gcttctctcc
     3121 atggtgttaa aacaatgact gaaagcgtct tgggtagggc aagggtatat ttagcttata
     3181 gttgcacatg acagtccatc gtcaagggag agcatggcgg gaactcaagg actttgagga
     3241 ggaactgaag cacaggcata gaggctgttc ctcactggct tgctccccag ggcttgctca
     3301 gctagctgtc ttacagagcc cagaaccccg atgcccttga gagccttcaa cccagtcagc
     3361 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     3421 atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct
     3481 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3541 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3601 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3661 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3721 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3781 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3841 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3901 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3961 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4021 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4081 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4141 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4201 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4261 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4321 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4381 ttggtctgac agcggccgca aatgctaaac cactgcagtg gttaccagtg cttgatcagt
     4441 gaggcaccga tctcagcgat ctgcctattt cgttcgtcca tagtggcctg actccccgtc
     4501 gtgtagatca ctacgattcg tgagggctta ccatcaggcc ccagcgcagc aatgatgccg
     4561 cgagagccgc gttcaccggc ccccgatttg tcagcaatga accagccagc agggagggcc
     4621 gagcgaagaa gtggtcctgc tactttgtcc gcctccatcc agtctatgag ctgctgtcgt
     4681 gatgctagag taagaagttc gccagtgagt agtttccgaa gagttgtggc cattgctact
     4741 ggcatcgtgg tatcacgctc gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg
     4801 tcaagccggg tcacatgatc acccatatta tgaagaaatg cagtcagctc cttagggcct
     4861 ccgatcgttg tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta
     4921 cacaattctc ttaccgtcat gccatccgta agatgctttt ccgtgaccgg cgagtactca
     4981 accaagtcgt tttgtgagta gtgtatacgg cgaccaagct gctcttgccc ggcgtctata
     5041 cgggacaaca ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct
     5101 tcggggcgga aagactcaag gatcttgccg ctattgagat ccagttcgat atagcccact
     5161 cttgcaccca gttgatcttc agcatctttt actttcacca gcgtttcggg gtgtgcaaaa
     5221 acaggcaagc aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg ttggatgctc
     5281 atactcgtcc tttttcaata ttattgaagc atttatcagg gttactagta cgtctctcaa
     5341 ggataagtaa gtaatattaa ggtacgggag gtattggaca ggccgcaata aaatatcttt
     5401 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
     5461 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
     5521 aggtgccaga acatttctct ggcctaactg gccggtacct gagctcgcta gcctcgagga
     5581 tatc
//
