LOCUS       sequence-322408-        5428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter for Chantico lncRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Hall JM, Kainth AS, Rowton MJ, Lu E, Haddad G, Keplinger AJ, 
            Scepanovic J , Perez-Cervantes C, Chan SSK, Piccirilli JA, Moskowitz
            IP, Ruthenburg AJ
  TITLE     The cardiac lncRNA Chantico directly regulates Cxcl1 chemokine 
            transcription
  JOURNAL   bioRxiv 2021.12.23.474040
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.12.23.474040"
COMMENT     Sequence Label: pChantico-Firefly
FEATURES             Location/Qualifiers
     source          1..5428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1178..1209
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1236..1245
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1242..2894
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2938..3059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2949..2968)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3003..3022
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3307..3324)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3478..4066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3558..3577)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4266..5057,5058..5126))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5231..5279
                     /note="synthetic polyadenylation signal"
     misc_feature    5293..5384
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5333..5352
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gataaggatc cggtcattca gtaaatataa atgcagatag cctacgttct gaaagtgggg
       61 ttctagggtc ccttggttag ttttaaaaat tcaaagtcac tttgagtttt gttttgtttt
      121 gttttgtttt aaagcttgag agtggccgtg cagagcaaag ttgtccaatt aagtttatat
      181 cagtgacaga tgggcttatc agaccttcgc tctcagacag tagccatgga acacttagct
      241 gctgcctccc gaaagtgcaa gcagaccagt tcaggatctg aagctttgtt ttggtttgac
      301 ttaaatgtaa gtgcaaacag tcgctgtggg gtgtgtgtgt gtgtgtgtgt gtgatactgg
      361 ccgatgcagg ctgagagtag gggtgtctca ctcctgactg cgtactcccc ctctgtttta
      421 cacaggctta tttactgtta cacggagact tttcttcgaa ctgttaaact cttggctgag
      481 tttgtgtgga gtatgaggca aatctctaac cgcaagcatg cagtggaaag attaggcata
      541 cttttgaaac gagtttgtgt accagatcat attcttggct caagaaagaa aaagaaaggg
      601 ggctggggaa aggcttgtgg ttgctactct gggaactaaa gaattcaaaa ggcttgttct
      661 ccatgtttcg tagacacaga catctggaat tgttgtttta aaattacaag caattgtgta
      721 tgctttgtta ctgcaacact tattcagagg caaggacata ggtgccactg ttgtcagaaa
      781 gccagatgtc ctttccttaa attggcctgc ctggctgctg gcgcctgggt gtcagcagac
      841 actttgggaa tctcagttag cggtactgca tggatatagt gcatctgctt ctcacacttc
      901 tgccaacctt gtgtattggt ttgcttctct ccatggtgtt aaaacaatga ctgaaagcgt
      961 cttgggtagg gcaagggtat atttagctta tagttgcaca tgacagtcca tcgtcaaggg
     1021 agagcatggc gggaactcaa ggactttgag gaggaactga agcacaggca tagaggctgt
     1081 tcctcactgg cttgctcccc agggcttgct cagctagctg tcttacagag cccagaaccg
     1141 atatcaagat ctggcctcgg cggccaagct tagacactag agggtatata atggaagctc
     1201 gacttccagc ttggcaatcc ggtactgttg gtaaagccac catggaagat gccaaaaaca
     1261 ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc
     1321 acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata
     1381 tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta
     1441 tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc
     1501 agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg
     1561 acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat
     1621 tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac
     1681 aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct
     1741 tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg
     1801 accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg
     1861 gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct
     1921 tcggcaacca gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct
     1981 tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc
     2041 gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc
     2101 tggtgcccac actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa
     2161 gcaacttgca cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg
     2221 tggccaaacg cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca
     2281 gcgccattct gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc
     2341 ccttcttcga ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc
     2401 gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg
     2461 ctacaaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg
     2521 acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct
     2581 accaggtagc cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg
     2641 ccggggtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc
     2701 tggaacacgg taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta
     2761 caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga
     2821 ccggcaagtt ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca
     2881 agatcgccgt gtaataattc tagagtcggg gcggccggcc gcttcgagca gacatgataa
     2941 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     3001 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     3061 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     3121 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgatgccc
     3181 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     3241 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc
     3301 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3361 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3421 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3481 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3541 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3601 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3661 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3721 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3781 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3841 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3901 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3961 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4021 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4081 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4141 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4201 aatctaaagt atatatgagt aaacttggtc tgacagcggc cgcaaatgct aaaccactgc
     4261 agtggttacc agtgcttgat cagtgaggca ccgatctcag cgatctgcct atttcgttcg
     4321 tccatagtgg cctgactccc cgtcgtgtag atcactacga ttcgtgaggg cttaccatca
     4381 ggccccagcg cagcaatgat gccgcgagag ccgcgttcac cggcccccga tttgtcagca
     4441 atgaaccagc cagcagggag ggccgagcga agaagtggtc ctgctacttt gtccgcctcc
     4501 atccagtcta tgagctgctg tcgtgatgct agagtaagaa gttcgccagt gagtagtttc
     4561 cgaagagttg tggccattgc tactggcatc gtggtatcac gctcgtcgtt cggtatggct
     4621 tcgttcaact ctggttccca gcggtcaagc cgggtcacat gatcacccat attatgaaga
     4681 aatgcagtca gctccttagg gcctccgatc gttgtcagaa gtaagttggc cgcggtgttg
     4741 tcgctcatgg taatggcagc actacacaat tctcttaccg tcatgccatc cgtaagatgc
     4801 ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg agtagtgtat acggcgacca
     4861 agctgctctt gcccggcgtc tatacgggac aacaccgcgc cacatagcag tactttgaaa
     4921 gtgctcatca tcgggaatcg ttcttcgggg cggaaagact caaggatctt gccgctattg
     4981 agatccagtt cgatatagcc cactcttgca cccagttgat cttcagcatc ttttactttc
     5041 accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt
     5101 gcgacacgaa aatgttggat gctcatactc gtcctttttc aatattattg aagcatttat
     5161 cagggttact agtacgtctc tcaaggataa gtaagtaata ttaaggtacg ggaggtattg
     5221 gacaggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
     5281 atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa
     5341 ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctggccta actggccggt
     5401 acctgagctc gctagcctcg aggatatc
//
