LOCUS       sequence-322409-        5420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter for Chantico lncRNA with Tbx5 site deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Hall JM, Kainth AS, Rowton MJ, Lu E, Haddad G, Keplinger AJ, 
            Scepanovic J , Perez-Cervantes C, Chan SSK, Piccirilli JA, Moskowitz
            IP, Ruthenburg AJ
  TITLE     The cardiac lncRNA Chantico directly regulates Cxcl1 chemokine 
            transcription
  JOURNAL   bioRxiv 2021.12.23.474040
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.12.23.474040"
COMMENT     Sequence Label: pChantico-Tdel-Firefly
FEATURES             Location/Qualifiers
     source          1..5420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1170..1201
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1228..1237
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1234..2886
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2930..3051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2941..2960)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2995..3014
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3299..3316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3470..4058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3550..3569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4258..5049,5050..5118))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5223..5271
                     /note="synthetic polyadenylation signal"
     misc_feature    5285..5376
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5325..5344
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gataaggatc cggtcattca gtaaatataa atgcagatag cctacgttct gaaagtgggg
       61 ttctagggtc ccttggttag ttttaaaaat tcaaagtcac tttgagtttt gttttgtttt
      121 gttttgtttt aaagcttgag agtggccgtg cagagcaaag ttgtccaatt aagtttatat
      181 cagtgacaga tgggcttatc agaccttcgc tctcagacag tagccatgga acacttagct
      241 gctgcctccc gaaagtgcaa gcagaccagt tcaggatctg aagctttgtt ttggtttgac
      301 ttaaatgtaa gtgcaaacag tcgctgtggg gtgtgtgtgt gtgtgtgtgt gtgatactgg
      361 ccgatgcagg ctgagagtag gggtgtctca ctcctgactg cgtactcccc ctctgtttta
      421 cacaggctta tttactgtta cacggagact tttcttcgaa ctgttaaact cttggctgag
      481 tttgtgtgga gtatgaggca aatctctaac cgcaagcatg cagtggaaag attaggcata
      541 cttttgaaac gagtttgtgt accagatcat attcttggct caagaaagaa aaagaaaggg
      601 ggctggggaa aggcttgtgg ttgctactct gggaactaaa gaattcaaaa ggcttgttct
      661 ccatgtttcg tagacacaga catctggaat tgttgtttta aaattacaag caattgtgta
      721 tgctttgtta ctgcaacact tattcagagg caaggacata ggtgccactg ttgtcagaaa
      781 gccagatgtc ctttccttaa attggcctgc ctggctgctg gcgcctgcag acactttggg
      841 aatctcagtt agcggtactg catggatata gtgcatctgc ttctcacact tctgccaacc
      901 ttgtgtattg gtttgcttct ctccatggtg ttaaaacaat gactgaaagc gtcttgggta
      961 gggcaagggt atatttagct tatagttgca catgacagtc catcgtcaag ggagagcatg
     1021 gcgggaactc aaggactttg aggaggaact gaagcacagg catagaggct gttcctcact
     1081 ggcttgctcc ccagggcttg ctcagctagc tgtcttacag agcccagaac cgatatcaag
     1141 atctggcctc ggcggccaag cttagacact agagggtata taatggaagc tcgacttcca
     1201 gcttggcaat ccggtactgt tggtaaagcc accatggaag atgccaaaaa cattaagaag
     1261 ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc
     1321 atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg
     1381 gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc
     1441 tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc
     1501 atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac
     1561 aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc
     1621 aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc
     1681 atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact
     1741 tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac
     1801 aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc
     1861 ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac
     1921 cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg
     1981 ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag
     2041 gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc
     2101 acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg
     2161 cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa
     2221 cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt
     2281 ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc
     2341 gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag
     2401 ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac
     2461 gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac
     2521 gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta
     2581 gccccagccg aactggagag catcctgctg caacacccca acatcttcga cgccggggtc
     2641 gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac
     2701 ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc
     2761 aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag
     2821 ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc
     2881 gtgtaataat tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt
     2941 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     3001 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     3061 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     3121 taaaacctct acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc
     3181 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     3241 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct
     3301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3901 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4141 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4201 gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta
     4261 ccagtgcttg atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt
     4321 ggcctgactc cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag
     4381 cgcagcaatg atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca
     4441 gccagcaggg agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc
     4501 tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt
     4561 tgtggccatt gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa
     4621 ctctggttcc cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt
     4681 cagctcctta gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat
     4741 ggtaatggca gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt
     4801 gaccggcgag tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc
     4861 ttgcccggcg tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat
     4921 catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag
     4981 ttcgatatag cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt
     5041 ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg
     5101 aaaatgttgg atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta
     5161 ctagtacgtc tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc
     5221 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     5281 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
     5341 tccccagtgc aagtgcaggt gccagaacat ttctctggcc taactggccg gtacctgagc
     5401 tcgctagcct cgaggatatc
//
