LOCUS       sequence-322410-        6142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter for Chantico lncRNA with triple pAS insertion 
            downstream of the promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Hall JM, Kainth AS, Rowton MJ, Lu E, Haddad G, Keplinger AJ, 
            Scepanovic J , Perez-Cervantes C, Chan SSK, Piccirilli JA, Moskowitz
            IP, Ruthenburg AJ
  TITLE     The cardiac lncRNA Chantico directly regulates Cxcl1 chemokine 
            transcription
  JOURNAL   bioRxiv 2021.12.23.474040
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.12.23.474040"
COMMENT     Sequence Label: pChantico-pAS-Firefly
FEATURES             Location/Qualifiers
     source          1..6142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1168..1216
                     /note="synthetic polyadenylation signal"
     polyA_signal    1225..1359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1368..1829
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     promoter        1892..1923
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1950..1959
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1956..3608
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3652..3773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4021..4038)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4192..4780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4272..4291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4980..5771,5772..5840))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5945..5993
                     /note="synthetic polyadenylation signal"
     misc_feature    6007..6098
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6047..6066
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gataaggatc cggtcattca gtaaatataa atgcagatag cctacgttct gaaagtgggg
       61 ttctagggtc ccttggttag ttttaaaaat tcaaagtcac tttgagtttt gttttgtttt
      121 gttttgtttt aaagcttgag agtggccgtg cagagcaaag ttgtccaatt aagtttatat
      181 cagtgacaga tgggcttatc agaccttcgc tctcagacag tagccatgga acacttagct
      241 gctgcctccc gaaagtgcaa gcagaccagt tcaggatctg aagctttgtt ttggtttgac
      301 ttaaatgtaa gtgcaaacag tcgctgtggg gtgtgtgtgt gtgtgtgtgt gtgatactgg
      361 ccgatgcagg ctgagagtag gggtgtctca ctcctgactg cgtactcccc ctctgtttta
      421 cacaggctta tttactgtta cacggagact tttcttcgaa ctgttaaact cttggctgag
      481 tttgtgtgga gtatgaggca aatctctaac cgcaagcatg cagtggaaag attaggcata
      541 cttttgaaac gagtttgtgt accagatcat attcttggct caagaaagaa aaagaaaggg
      601 ggctggggaa aggcttgtgg ttgctactct gggaactaaa gaattcaaaa ggcttgttct
      661 ccatgtttcg tagacacaga catctggaat tgttgtttta aaattacaag caattgtgta
      721 tgctttgtta ctgcaacact tattcagagg caaggacata ggtgccactg ttgtcagaaa
      781 gccagatgtc ctttccttaa attggcctgc ctggctgctg gcgcctgggt gtcagcagac
      841 actttgggaa tctcagttag cggtactgca tggatatagt gcatctgctt ctcacacttc
      901 tgccaacctt gtgtattggt ttgcttctct ccatggtgtt aaaacaatga ctgaaagcgt
      961 cttgggtagg gcaagggtat atttagctta tagttgcaca tgacagtcca tcgtcaaggg
     1021 agagcatggc gggaactcaa ggactttgag gaggaactga agcacaggca tagaggctgt
     1081 tcctcactgg cttgctcccc agggcttgct cagctagctg tcttacagag cccagaaccg
     1141 aggttctctt ttcccagttg tctctagaat aaaagatctt tattttcatt agatctgtgt
     1201 gttggttttt tgtgtggggg cccgaacttg tttattgcag cttataatgg ttacaaataa
     1261 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     1321 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc atcgatgctg taagtctgca
     1381 gaaattgatg atctattaaa caataaagat gtccactaaa atggaagttt ttcctgtcat
     1441 actttgttaa gaagggtgag aacagagtac ctacattttg aatggaagga ttggagctac
     1501 gggggtgggg gtggggtggg attagataaa tgcctgctct ttactgaagg ctctttacta
     1561 ttgctttatg ataatgtttc atagttggat atcataattt aaacaagcaa aaccaaatta
     1621 agggccagct cattcctccc actcatgatc tatagatcta tagatctctc gtgggatcat
     1681 tgtttttctc ttgattccca ctttgtggtt ctaagtactg tggtttccaa atgtgtcagt
     1741 ttcatagcct gaagaacgag atcagcagcc tctgttccac atacacttca ttctcagtat
     1801 tgttttgcca agttctaatt ccatcagaac ctcgagttta aacgtgaagt tccgatatca
     1861 agatctggcc tcggcggcca agcttagaca ctagagggta tataatggaa gctcgacttc
     1921 cagcttggca atccggtact gttggtaaag ccaccatgga agatgccaaa aacattaaga
     1981 agggcccagc gccattctac ccactcgaag acgggaccgc cggcgagcag ctgcacaaag
     2041 ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt taccgacgca catatcgagg
     2101 tggacattac ctacgccgag tacttcgaga tgagcgttcg gctggcagaa gctatgaagc
     2161 gctatgggct gaatacaaac catcggatcg tggtgtgcag cgagaatagc ttgcagttct
     2221 tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct aacgacatct
     2281 acaacgagcg cgagctgctg aacagcatgg gcatcagcca gcccaccgtc gtattcgtga
     2341 gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga
     2401 tcatcatcat ggatagcaag accgactacc agggcttcca aagcatgtac accttcgtga
     2461 cttcccattt gccacccggc ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg
     2521 acaaaaccat cgccctgatc atgaacagta gtggcagtac cggattgccc aagggcgtag
     2581 ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca
     2641 accagatcat ccccgacacc gctatcctca gcgtggtgcc atttcaccac ggcttcggca
     2701 tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg
     2761 aggaggagct attcttgcgc agcttgcaag actataagat tcaatctgcc ctgctggtgc
     2821 ccacactatt tagcttcttc gctaagagca ctctcatcga caagtacgac ctaagcaact
     2881 tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca
     2941 aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca accagcgcca
     3001 ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct
     3061 tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac cagcgcggcg
     3121 agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaacccc gaggctacaa
     3181 acgctctcat cgacaaggac ggctggctgc acagcggcga catcgcctac tgggacgagg
     3241 acgagcactt cttcatcgtg gaccggctga agagcctgat caaatacaag ggctaccagg
     3301 tagccccagc cgaactggag agcatcctgc tgcaacaccc caacatcttc gacgccgggg
     3361 tcgccggcct gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac
     3421 acggtaaaac catgaccgag aaggagatcg tggactatgt ggccagccag gttacaaccg
     3481 ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga ctgaccggca
     3541 agttggacgc ccgcaagatc cgcgagattc tcattaaggc caagaagggc ggcaagatcg
     3601 ccgtgtaata attctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca
     3661 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     3721 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     3781 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     3841 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga
     3901 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     3961 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc
     4021 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4081 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4141 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4201 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4261 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4321 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4381 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4441 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4501 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4561 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4621 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4681 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4741 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4801 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4861 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4921 aagtatatat gagtaaactt ggtctgacag cggccgcaaa tgctaaacca ctgcagtggt
     4981 taccagtgct tgatcagtga ggcaccgatc tcagcgatct gcctatttcg ttcgtccata
     5041 gtggcctgac tccccgtcgt gtagatcact acgattcgtg agggcttacc atcaggcccc
     5101 agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc agcaatgaac
     5161 cagccagcag ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc ctccatccag
     5221 tctatgagct gctgtcgtga tgctagagta agaagttcgc cagtgagtag tttccgaaga
     5281 gttgtggcca ttgctactgg catcgtggta tcacgctcgt cgttcggtat ggcttcgttc
     5341 aactctggtt cccagcggtc aagccgggtc acatgatcac ccatattatg aagaaatgca
     5401 gtcagctcct tagggcctcc gatcgttgtc agaagtaagt tggccgcggt gttgtcgctc
     5461 atggtaatgg cagcactaca caattctctt accgtcatgc catccgtaag atgcttttcc
     5521 gtgaccggcg agtactcaac caagtcgttt tgtgagtagt gtatacggcg accaagctgc
     5581 tcttgcccgg cgtctatacg ggacaacacc gcgccacata gcagtacttt gaaagtgctc
     5641 atcatcggga atcgttcttc ggggcggaaa gactcaagga tcttgccgct attgagatcc
     5701 agttcgatat agcccactct tgcacccagt tgatcttcag catcttttac tttcaccagc
     5761 gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat gagtgcgaca
     5821 cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt attgaagcat ttatcagggt
     5881 tactagtacg tctctcaagg ataagtaagt aatattaagg tacgggaggt attggacagg
     5941 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     6001 agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc
     6061 tgtccccagt gcaagtgcag gtgccagaac atttctctgg cctaactggc cggtacctga
     6121 gctcgctagc ctcgaggata tc
//
