LOCUS       sequence-322416-        7865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses in Candida albicans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Van Genechten W, Demuyser L, Dedecker P, Van Dijck P
  TITLE     Presenting a codon-optimized palette of fluorescent proteins for use
            in Candida albicans.
  JOURNAL   Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.1038/s41598-020-63308-w.
  PUBMED    32273559
REFERENCE   2  (bases 1 to 7865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-04-9"; volume: "10(1):6158. doi"; pages: " 
            10.1038/s41598-020-63308-w"
COMMENT     Sequence Label: CIp10 - mScarlet-I IDT
FEATURES             Location/Qualifiers
     source          1..7865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(725..741)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(726..742)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(1151..1169)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1190..1206)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1190..1206)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1203..1225)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1214..1230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1238..1244,1245..1262,1263..1268))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1283..1304
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1421..1438)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1592..2180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1672..1691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2351..3142,3143..3211))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2974..2993
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3212..3316)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3343..3798
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3430..3449)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3640..3661
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3924..3946
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3938..3955
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3939..3955
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3962..3981
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3962..3980
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgctgcaga tggtcagtaa aggggaagca gtcatcaagg aatttatgcg ttttaaggtt
       61 cacatggaag gatctatgaa cggacatgaa tttgagatcg aaggtgaagg tgaagggcga
      121 ccatacgagg gaactcaaac cgccaaattg aaagtgacca agggtgggcc attgccattc
      181 tcatgggaca tcttgagtcc acaatttatg tatggaagtc gagcatttat caagcaccct
      241 gctgatatac ctgactacta taaacaatct ttccctgagg ggttcaagtg ggagagagtg
      301 atgaattttg aggatggtgg ggccgtaact gttacacagg atacatcact tgaggatgga
      361 acattaattt acaaggtaaa attgcgaggg accaactttc cacctgacgg acctgtaatg
      421 cagaaaaaaa ctatgggttg ggaggcttcc accgaacgat tgtacccaga agatggggtt
      481 cttaaaggtg atattaaaat ggccttgaga ttgaaagatg gaggaagata cttagccgac
      541 tttaaaacaa cctacaaagc taagaaacct gtgcaaatgc ctggggccta taacgtagac
      601 cgaaagttgg atataacctc tcataatgag gattatacag tggttgaaca gtatgaacga
      661 tctgagggac gacactctac tggaggaatg gatgagttat ataagtaact agtagcgacg
      721 tcatcgatac cgtcgacctc gaggagtgaa attctggaaa tctggaaatc tggttttgta
      781 ttcttgttat tcttcttttt gttattacat atataacttg ttactttttt aaaaaaatct
      841 ttgtttattt tataaatata taaaactaaa tttaagaaaa agagaaaaat gttttatttg
      901 agagattgaa attttacttg aatttagctt agcttttata aagtattatt atgtaaaaaa
      961 acaaaacaaa tatacattaa aaagttaaga ctataaaata gccacccaag gcatttctat
     1021 atcttgttgt tgttgttttc atcttctgta tcagaggaac ttattttatt attttcgtca
     1081 cgggtatttt ctcttgtttg atgattcatc ccattcattc catcaaaaat gtcggtacca
     1141 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
     1201 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1261 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1321 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1381 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1441 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1501 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1561 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1621 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1681 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1741 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1801 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1861 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1921 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1981 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2041 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2101 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2161 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2221 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2281 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2341 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2401 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2461 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2521 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2581 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2641 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2701 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2761 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2821 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2881 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2941 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3001 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3061 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3121 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3181 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3241 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3301 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
     3361 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3421 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3481 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3541 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3601 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3661 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3721 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     3781 tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     3841 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     3901 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
     3961 gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc gcgtttataa
     4021 taaacttagt ctaatatttc ttttggacca aaatagaaac tgttctcagg tggcaaaaaa
     4081 cagcttaaag tctgtgttaa aaaaagatct cggctatgcc aatcaaaaag gagtgacatt
     4141 attatgtaaa agaagaaatg ttgtaattcc gcgaaacaac aattttaatg cttactgtgc
     4201 cgccaccaat atcctatggc caaggggcct ttaaacatgc cacggaaata tacagtcaaa
     4261 gtaaacctac tatgtcttta gagccttcag gatcaattgt ttttaaatga atgactgcaa
     4321 aactagaaaa gtagcctctt acataagcaa gtcatttgta caccgagagt ttagtaacca
     4381 gtgaagcaag tatatctggc aaacctgaaa cttatctgtg aattataaag ctcataagta
     4441 aaaagtgtag aaaagaagat aaggcagacc aaatacaaat ttgcaacagt acaacgaata
     4501 tttcagcgtc aaaactagag aataataaag aaaacgatct tttcaaaaag aaaaaacctt
     4561 ttagttttcc tttgttgttg ttgtgggtgt gtgctattta tattatatag tttactcata
     4621 ataccataaa atattcggtt tgattaggtt attttaataa gctaatttgt ttctaatcgt
     4681 gtaatttatg ctgtgtatat taagtagtgt gtgcactgcc caaaaatgtt tgttgtttat
     4741 agtcggttaa agagaaaaaa gaaaaaaaga tccatacaca cacgttaatt agttgttcaa
     4801 cgtaatacac tcatattttg ttcttatttg ctttcggtcg ctgttctcac caagatttat
     4861 tgccaacgaa acaatttttt tttatatatt ttcagatttt tctttttttc ctttcctttc
     4921 cttttctaat tttcactcct ggttttcttt ctttcttaga aacattatct cgatattaat
     4981 attaaaaaaa tataatcatt caaactccgg aatgtctact actttggatg atactgctta
     5041 tagatacaga acttctgttc caggtgatgc tgaagctatt gaagctttgg atggttcttt
     5101 cactaccgat actgttttta gagttactgc tactggtgat ggtttcactt tgagagaagt
     5161 tccagttgat ccaccattga ctaaggtttt cccagatgat gaatccgatg atgaatccga
     5221 tgctggtgaa gatggtgatc cagattctag aactttcgtt gcttatggtg atgatggtga
     5281 tttggctggt ttcgttgttg tttcttattc tggttggaac agaagattga ctgttgaaga
     5341 tattgaagtt gctccagaac atagaggtca tggtgttggt agagctttga tgggtttggc
     5401 tactgaattc gccagagaaa gaggtgctgg tcatttgtgg ttggaagtta ccaatgttaa
     5461 tgctccagct attcatgctt atagaagaat gggtttcact ttgtgtggtt tggatactgc
     5521 tttatacgat ggtactgctt ccgatggtga acaagctttg tatatgtcta tgccatgtcc
     5581 ataataactc ggggggatcc gagtgaaatt ctggaaatct ggaaatctgg ttttgtattc
     5641 ttgttattct tctttttgtt attacatata taacttgtta cttttttaaa aaaatctttg
     5701 tttattttat aaatatataa aactaaattt aagaaaaaga gaaaaatgtt ttatttgaga
     5761 gattgaaatt ttacttgaat ttagcttagc ttttataaag tattattatg taaaaaaaca
     5821 aaacaaatat acattaaaaa gttaagacta taaaatagcc acccaaggca tttctatatc
     5881 ttgttgttgt tgttttcatc ttctgtatca gaggaactta ttttattatt ttcgtcacgg
     5941 gtattttctc ttgtttgatg attcatccca ttcttccatc ataaaatact agtggatccc
     6001 ccagatcctt atccttaaac agattctaaa acaacatctt tgaaaccaga agaaactttc
     6061 ttaccttttt cttcggttga accttcaccg tgcaaagcca ataatgaacc caagtcgaat
     6121 tttggttgtt tcaacaattt gacttttctg atgtggacat tttgtaatgg gaaaatggtt
     6181 tgggtggatt tttcaatttc acggccaatg acttctggaa tcaatttgga agttaattga
     6241 gctaaagtac agttggaaac ttctctttgc ataatttcaa tcatcttctt tctgacttct
     6301 ctcaatttag aggattgagc gtaagtagtt ttcttgattt ggtttggttg tcttttggtg
     6361 aaagcaatgg caaaaactct caaaacgtaa tcgtcggaag ttttaacagt gacattagct
     6421 tcaactaatg attgccattt tctgaccaat gatcttaatt tgtcagaagt gaaatccaaa
     6481 ccatgaggcc tccatgaggc ctccatgagg cctccatgag gcctccatgg aggcctcatg
     6541 gaagttggtc aacaagtttt taccttggac ttcatcaact ctcaatttga tttttctgta
     6601 agagtggtct tcggaacctt gtaagtcggc caaacaaact tcgaaaactc tacctttcaa
     6661 gccatcagcg gcattcttta aaccggtaga tctgttgatc aaagttttac caacatttct
     6721 gttttcaaaa gtggttggag ctttgatgtc aaaccaatct tttctggtga atgggtcaac
     6781 gaccttcttt tttaatcctt tctttccttt ggacaatctc ttgtttttac cgacagccat
     6841 gttgtacttg agttggatct acgcgtcagc ctcgtttata ataaacttag tctaatattt
     6901 cttttggacc aaaatagaaa ctgttctcag gtggcaaaaa acagcttaaa gtctgtgtta
     6961 aaaaaagatc tcggctatgc caatcaaaaa ggagtgacat tattatgtaa aagaagaaat
     7021 gttgtaattc cgcgaaacaa caattttaat gcttactgtg ccgccaccaa tatcctatgg
     7081 ccaaggggcc tttaaacatg ccacggaaat atacagtcaa agtaaaccta ctatgtcttt
     7141 agagccttca ggatcaattg tttttaaatg aatgactgca aaactagaaa agtagcctct
     7201 tacataagca agtcatttgt acaccgagag tttagtaacc agtgaagcaa gtatatctgg
     7261 caaacctgaa acttatctgt gaattataaa gctcataagt aaaaagtgta gaaaagaaga
     7321 taaggcagac caaatacaaa tttgcaacag tacaacgaat atttcagcgt caaaactaga
     7381 gaataataaa gaaaacgatc ttttcaaaaa gaaaaaacct tttagttttc ctttgttgtt
     7441 gttgtgggtg tgtgctattt atattatata gtttactcat aataccataa aatattcggt
     7501 ttgattaggt tattttaata agctaatttg tttctaatcg tgtaatttat gctgtgtata
     7561 ttaagtagtg tgtgcactgc ccaaaaatgt ttgttgttta tagtcggtta aagagaaaaa
     7621 agaaaaaaag atccatacac acacgttaat tagttgttca acgtaataca ctcatatttt
     7681 gttcttattt gctttcggtc gctgttctca ccaagattta ttgccaacga aacaattttt
     7741 ttttatatat tttcagattt ttcttttttt cctttccttt ccttttctaa ttttcactcc
     7801 tggttttctt tctttcttag aaacattatc tcgatattaa tattaaaaaa atataatcat
     7861 tcaaa
//
