LOCUS       sequence-322448-       12044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags 
            in a mammalian system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   Ko M, Huang Y, Jankowska AM, Pape UJ, Tahiliani M, Bandukwala HS, An
            J, Lamperti ED, Koh KP, Ganetzky R, Liu XS, Aravind L, Agarwal S, 
            Maciejewski JP, Rao A
  TITLE     Impaired hydroxylation of 5-methylcytosine in myeloid cancers with 
            mutant TET2.
  JOURNAL   Nature. 2010 Dec 9;468(7325):839-43. doi: 10.1038/nature09586.
  PUBMED    21057493
REFERENCE   2  (bases 1 to 12044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-9- 9"; volume: "468"; issue: "7325"; pages:
            "839-43"
COMMENT     Sequence Label: FH-Tet2-pEF
FEATURES             Location/Qualifiers
     source          1..12044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(166..185)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        473..1651
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          704..1642
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1599..1619
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1668..1687
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1668..1686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1784..1807
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1820..1846
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1820..1846
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7675..7716
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7726..7743
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7766..7783)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7772..7996
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8042..8470
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8129..8148)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8339..8360
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8479..8499)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8484..8813
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8664..8799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8726..8745
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8880..9674
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8934..8953)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9544..9563
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9850..9971
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9887..9906)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9941..9960
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10020..10036)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10020..10036)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10033..10055)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10044..10060
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10068..10074,10075..10092,10093..10098))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10113..10134
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10251..10268)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10411..10999)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10491..10510)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11170..11961,11962..12030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11793..11812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(12031..12044,1..91))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag
     1741 tgtggtggaa ttcgccctta gatcttccgc tcgatgaacc atggactaca aggacgacga
     1801 tgacaagctc gatggaggat acccctacga cgtgcccgac tacgccggag gactcgagga
     1861 acaggacaga accacccatg ctgagggcac cagactgagt ccattcctga tagcaccacc
     1921 ttctcccatc agccatacag aacctctggc tgtcaaactc cagaatggaa gcccgttagc
     1981 agagagacct catccagaag taaatggaga caccaagtgg caatcttccc aaagctgtta
     2041 tggaatatcc cacatgaaag gcagccagag cagtcatgag agtccacatg aagacagagg
     2101 gtattccagg tgcttacaga atggagggat aaaacgcaca gtcagtgaac catctctctc
     2161 tgggctccat ccaaacaaga tattgaaact agaccaaaaa gctaagggag aaagcaatat
     2221 cttcgaggaa agccaagaaa gaaaccacgg taaaagcagc cgtcagccaa atgtctccgg
     2281 actaagtgat aatggagagc ccgtgacctc gaccacccag gaaagttcag gtgcagatgc
     2341 tttcccaaca cggaactaca atggagttga aattcaggtt ctcaacgagc aggaagggga
     2401 aaaaggcagg agcgttacat tacttaaaaa caagattgtg ctaatgccta atggtgctac
     2461 agtttctgcc cattctgagg agaacacacg tggtgaactc ctggaaaaaa cacagtgtta
     2521 tccagattgt gtctccattg ccgtgcagag taccgcatct catgtaaaca cccctagcag
     2581 tcaggctgct atcgagttgt cccacgagat ccctcaacca tcgcttacct cagcgcagat
     2641 caatttctcc cagacctcaa gcttgcagct gcctccagag ccagctgcaa tggtgactaa
     2701 ggcctgtgat gctgataatg ccagtaaacc agctatagta ccaggtacct gtccttttca
     2761 gaaagcagaa caccaacaaa agtcagcttt ggacataggc ccatctcgtg cagaaaacaa
     2821 aaccatccaa ggaagcatgg agctatttgc tgaagaatac tatcctagtt ccgaccggaa
     2881 tttgcaagct tcgcatggca gctctgaaca gtattcaaag caaaaggaaa ccaatggtgc
     2941 ttacttcagg caaagctcga agttccctaa agattccatc tctcccacta ctgtgacccc
     3001 accgtcacaa tcacttcttg ctccccgtct tgttcttcag cctcctttag aaggaaaagg
     3061 cgctctaaat gatgtagctt tggaagaaca ccatgactac cccaaccgaa gcaaccgaac
     3121 tcttttaagg gaagggaaaa tagaccatca acccaagaca tcatctagcc agagtctgaa
     3181 tccatctgta catacaccca accccccctt gatgcttcca gaacagcatc agaatgattg
     3241 tggctcaccg agccctgaaa agtcaagaaa aatgtcagaa tatctcatgt attacctgcc
     3301 aaatcatggc cacagtggag gtttacaaga acatagccaa tacctgatgg ggcacaggga
     3361 gcaagagatt ccgaaggatg caaacgggaa acaaacgcaa ggctctgtac aggcagcacc
     3421 tggctggata gaactgaaag ccccgaattt gcatgaagca ctccatcaga caaaacgcaa
     3481 ggatatatcc ttgcactcag tcctccactc tcagaccggc cctgtcaatc agatgagctc
     3541 caaacagtcc actggcaatg tcaacatgcc aggaggattc caaaggctac cttacctcca
     3601 gaaaacagcc cagccagagc agaaggcaca aatgtaccaa gtgcaagtga accaaggacc
     3661 gtctccaggt atgggggacc aacatcttca gttccagaaa gctttatacc aggagtgcat
     3721 ccccaggaca gatccgtcat ctgaggctca cccgcaagca ccgagcgttc ctcagtatca
     3781 tttccagcaa agagtaaatc cctccagtga taagcatttg agtcaacagg ccacagagac
     3841 tcaacggtta tcaggctttt tacaacatac tcctcagacg caggcatcac aaacaccagc
     3901 atcccagaac tcaaatttcc ctcaaatctg ccagcagcag cagcagcagc agttacagag
     3961 gaagaataaa gagcaaatgc ctcagacttt ctctcatctc caaggtagca atgataagca
     4021 aagagaaggc tcgtgctttg gccagattaa agtggaagaa agcttttgtg tcggaaatca
     4081 gtactccaaa tcaagtaatt tccaaactca caataatacc caaggggggt tggagcaagt
     4141 acaaaatata aataaaaatt ttccttattc gaagatctta acaccaaatt cgagcaactt
     4201 acagattctc ccttcaaatg acacacaccc ggcttgtgag cgggaacaag ctctacatcc
     4261 cgtaggaagt aagacctcaa acctgcagaa catgcagtat ttcccgaata atgtgacccc
     4321 aaatcaggac gttcaccggt gctttcagga acaagcgcag aagcctcagc aagcttcgtc
     4381 tctacagggg cttaaggaca gaagccaggg tgagtctcca gccccaccag ctgaggcagc
     4441 tcaacagagg tatttggtgc ataatgaagc aaaggcactc cctgtgcctg agcaaggagg
     4501 aagtcagaca cagacccctc ctcagaagga cactcagaag cacgctgcct taaggtggct
     4561 tctcttacag aagcaagaac agcagcaaac acagcaatcc cagcctggtc ataaccagat
     4621 gcttaggcca atcaagactg agcctgtatc caaaccttct tcctatagat accccttgtc
     4681 accgccacaa gaaaatatgt ccagcaggat aaagcaagag atctcctctc caagccgtga
     4741 caatgggcag ccaaagagca tcattgagac catggaacag cacctgaagc agtttcagct
     4801 caagtcactc tgtgactata aggctctgac tctcaagtca cagaaacacg tgaaagtgcc
     4861 aacagatatc caggctgcag aatcggagaa ccacgcccga gctgcagagc ctcaagcaac
     4921 caaaagcaca gattgttctg ttctcgacga tgtttcagaa tcagatactc ctggtgaaca
     4981 aagtcagaat ggcaaatgtg aaggatgcaa tccagacaaa gatgaagctc cttattatac
     5041 ccatctagga gctggtcctg atgtggcagc tattagaaca ctcatggaag aaaggtatgg
     5101 agagaagggt aaagctatta ggattgaaaa agtcatatat actggtaaag aaggcaagag
     5161 ctctcaggga tgtcctattg ctaaatgggt atatcggaga tcgagtgagg aggagaaact
     5221 actgtgtttg gtacgagtgc gacctaatca cacatgtgag acggcggtga tggtaattgc
     5281 catcatgttg tgggacggaa tcccaaagct actcgcatca gaactctact cagaacttac
     5341 agatatcttg ggcaagtgtg gcatatgcac caaccgtcgc tgttctcaga atgaaacgaa
     5401 gaaaaagcaa tcaccaccca gaaactgttg ttgtcagggt gagaatccag agacctgtgg
     5461 tgcctccttt tcttttggtt gttcttggag catgtactat aatggatgta agtttgccag
     5521 aagcaagaaa ccaaggaaat ttaggctaca tggagctgag ccaaaagagg aagagagact
     5581 aggttctcat ttgcaaaacc tggctactgt cattgctcca atatacaaga agcttgcacc
     5641 cgatgcatac aataatcagg ttgaatttga acaccaagcc ccagactgct gtttgggtct
     5701 gaaggaaggc cggccattct caggagtcac tgcatgtttg gacttctctg ctcattccca
     5761 cagagaccag cagaacatgc caaatggcag tacagtggtg gtcaccctca atagagaaga
     5821 caatcgagaa gtcggagcta agcctgagga tgagcagttc cacgtgctgc ctatgtacat
     5881 catcgcccct gaggatgagt ttgggagtac ggaaggccag gagaagaaga tacggatggg
     5941 gtccattgag gttctgcagt catttcggag gagaagggtc ataaggatag gagagctgcc
     6001 caagagttgc aagaagaaag cggagcccaa gaaagccaag accaagaaag cagctcgaaa
     6061 gcgttcctct ctggagaact gctccagtag gactgagaag ggaaagtctt cctcacatac
     6121 aaagctgatg gaaaatgcaa gccatatgaa acaaatgaca gcacaaccgc agctttcggg
     6181 cccggtcatc cggcagccac caacactcca gaggcacctt cagcaagggc agaggccaca
     6241 gcagccgcag ccacctcagc cgcagccgca gacgacacct cagccacagc cacagccaca
     6301 gcatatcatg cccggtaact ctcagtctgt tggttctcat tgttctggat ccaccagtgt
     6361 ctacacgaga cagcctactc ctcacagtcc ttatcccagc tcagcacaca cctcagatat
     6421 ttatggagat accaaccatg tgaactttta ccccacttca tctcatgcct cgggttcata
     6481 tttgaatcct tctaattaca tgaaccccta ccttgggctt ttgaatcaga ataaccaata
     6541 tgcacctttt ccatacaatg ggagtgtgcc agtggacaat ggttcccctt tcttaggttc
     6601 ttattccccc caggctcagt ccagggatct acatagatat ccaaaccagg accatctcac
     6661 caatcagaac ttaccaccca tccacaccct tcaccaacag acgtttgggg acagtccctc
     6721 taagtactta agttatggaa accaaaatat gcagagagat gccttcacta ctaactccac
     6781 cctaaaacca aatgtacacc acctagcaac gttttctcct taccccaccc ccaagatgga
     6841 tagtcatttc atgggagctg cctccagatc accatacagc cacccacaca ctgactacaa
     6901 aaccagtgag catcatctac cctctcacac gatctacagc tacacggcag cagcttcggg
     6961 gagcagttcc agccacgcct tccacaacaa ggagaatgac aacatagcca atgggctctc
     7021 aagagtgctt ccagggttta atcatgatag aactgcttct gcccaagaac tattatacag
     7081 tctgactggc agcagtcagg agaagcagcc tgaggtgtca ggccaggatg cagctgctgt
     7141 gcaggaaatt gagtattggt cagatagtga gcacaacttt caggatcctt gcattggagg
     7201 ggtggctata gctccaactc atgggtcaat tcttattgag tgtgcaaagt gtgaggttca
     7261 tgccacaacc aaagtaaacg atcccgaccg gaatcacccc accaggatct cacttgtact
     7321 gtataggcat aagaatttgt ttctaccaaa acattgtttg gctctctggg aagccaaaat
     7381 ggctgaaaag gcccggaaag aggaagagtg cggaaagaat ggatcagacc acgtgtctca
     7441 gaaaaatcat ggcaaacagg aaaagcgtga gcccacaggg ccacaggaac ccagttacct
     7501 gcgtttcatc cagtctcttg ctgagaacac agggtctgtg actacggatt ctaccgtgac
     7561 tacatcacca tatgctttca ctcaggtcac agggccttac aacacatttg tatgatagaa
     7621 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggccctt cgaaggtaag
     7681 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac
     7741 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     7801 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     7861 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     7921 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     7981 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     8041 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8101 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8161 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8221 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8281 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8341 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8401 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8461 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     8521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     8581 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8641 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8701 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     8761 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     8821 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     8881 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     8941 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     9001 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     9061 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     9121 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     9181 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     9241 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     9301 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     9361 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     9421 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     9481 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     9541 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     9601 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     9661 acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac
     9721 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     9781 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     9841 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9901 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9961 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
    10021 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    10081 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    10141 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    10201 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
    10261 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    10321 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    10381 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10441 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10501 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10561 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10621 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10681 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10741 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10801 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    10861 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10921 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10981 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    11041 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11101 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    11161 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    11221 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11281 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11341 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    11401 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    11461 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    11521 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    11581 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11641 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    11701 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    11761 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    11821 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    11881 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    11941 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    12001 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttc
//
