LOCUS       sequence-322456-        9508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH 
            system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: Str-Golgin84_VSVG-SBP-EGFP
FEATURES             Location/Qualifiers
     source          1..9508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(48..67)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        239..618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     773..793
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     867..886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        867..885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      937..946
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          3695..3924
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     3907..3926
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(3997..4571,4572..4574,4575..4583)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4164..4181)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4391..4410
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4597..4644,4645..6129)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     CDS             6139..6252
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             join(6262..6264,6265..6267,6268..6978)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6262..6264,6265..6267,6268..6978)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6307..6328)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6568..6587)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6915..6936
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7132..7253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7169..7188)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7355..7371)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7355..7371)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7368..7390)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7379..7395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7403..7409,7410..7427,7428..7433))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7448..7469
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7586..7603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7757..8345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7837..7856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8516..9307,9308..9376))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9139..9158
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9377..9481)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
       61 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
      121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
      181 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
      241 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      301 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      361 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      421 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      481 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      541 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      601 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      661 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      721 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      781 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
      841 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttgg
      901 taccgagctc ggatcgatat ctgcggccta gctagcgcca ccatggaccc tagcaaagac
      961 tcaaaagctc aggtgtccgc tgccgaggct ggcattactg gaacatggta caatcagctc
     1021 gggagcacct ttattgtgac tgctggagcc gatggagccc tcaccggaac atacgaatct
     1081 gctgtgggaa acgccgaatc acggtacgtc ctcactggcc gatacgatag tgcccctgcc
     1141 accgacggat ctgggactgc cctgggatgg actgtcgctt ggaaaaacaa ctaccggaat
     1201 gctcattctg ccacaacatg gagtggacag tacgtgggag gcgctgaggc tagaatcaat
     1261 acacagtggc tgctcacatc tggcacaacc gaggcaaatg cttggaaatc caccctggtg
     1321 ggacatgaca cattcaccaa agtgaaaccc tccgccgctt caatcgatgc cgccaaaaaa
     1381 gccggagtca acaacggcaa tcctctggat gccgtccagc agaccggttc ttggtttgtt
     1441 gatcttgctg gaaaggcaga agatctttta aaccgagttg atcaaggggc tgcaacagct
     1501 ctcagtagga aagacaatgc cagcaacata tatagcaaaa atactgacta tactgaactt
     1561 caccagcaaa atacagattt gatatatcag actggaccta aatctacgta tatttcatca
     1621 gcaggtgata acattcgaaa tcaaaaagcc accatcttag ctggcactgc aaatgtgaaa
     1681 gtaggatctc ggacaccagt agaggcctct catcctgttg aaaatgcatc tgttcctagg
     1741 ccttcatccc attttgtgcg aagaaaaaag tcagaacctg atgatgagct gctgtttgat
     1801 tttcttaata gttcacagaa ggagcctacc gggagggtgg aaatcagaaa ggaaaaaggc
     1861 aagacacctg tctttcagag ctctcagaca tcaagtgtca gttctgtgaa ccccagtgta
     1921 accaccatca aaaccattga agaaaattct tttgggagcc aaacccacga agctgccagt
     1981 aactcagatt ctagccatga aggtcaagag gaatcttcaa aggaaaatgt gtcatcaaat
     2041 gctgcctgcc ctgaccacac cccaacacct aatgatgatg gcaaatcaca tgaactgtct
     2101 aaccttcgac tggagaatca gctgctgagg aatgaagttc agtctttaaa tcaagaaatg
     2161 gcctcgttac tccaaagatc caaagagact caagaagaat taaacaaagc aagagcaaga
     2221 gttgaaaagt ggaatgctga ccattcaaag agtgatcgaa tgactcgagg actccgagcc
     2281 caagtagatg acctgactga agctgtggct gcaaaggatt cccagctggc tgtactgaaa
     2341 gtgagactcc aggaagctga ccagctactg agtactcgca cagaagcatt agaggcctta
     2401 cagagtgaaa aatcacgaat aatgcaggat caaagtgaag gtaacagcct gcagaatcaa
     2461 gctctgcaga ctcttcagga gagactgcat gaagcggatg ccactctgaa gagagagcag
     2521 gagagctata aacagatgca gagcgagttt gctgcacgcc ttaataaagt ggaaatggaa
     2581 cgtcagaatt tagcagaagc aattacactg gccgaaagaa aatactcaga tgagaagaag
     2641 agggttgatg aactgcagca gcaagtcaag ctgtataagt tgaacttgga gtcctctaag
     2701 caggaattaa ttgactacaa gcaaaaagct actagaatac tgcaatctaa ggaaaaattg
     2761 attaacagct tgaaagaagg ctctggtttt gaaggcctag atagcagcac tgccagtagc
     2821 atggagctgg aagaacttcg gcatgagaaa gagatgcaga gggaggaaat acagaagctg
     2881 atgggccaga tacatcagct cagatccgaa ttacaggata tggaggcaca gcaagttaat
     2941 gaagcagaat cagcaagaga acagttacag gatctgcatg accaaatagc tgggcagaaa
     3001 gcatccaaac aagaactaga gacagaactg gagcgactga agcaggagtt ccactatata
     3061 gaagaagatc tttatcgaac aaagaacaca ttgcaaagca gaattaaaga tcgagacgaa
     3121 gaaattcaaa aactcaggaa tcagcttacc aataaaactt taagcaatag cagtcagtct
     3181 gagttagaaa atcgactcca tcagctaaca gagactctca tccagaaaca gaccatgctg
     3241 gagagtctca gcacagaaaa gaactccctg gtctttcaac tggagcgcct cgaacagcag
     3301 atgaactccg cctctggaag tagtagtaat gggtcttcga ttaatatgtc tggaattgac
     3361 aatggtgaag gcactcgtct gcgaaatgtt cctgttcttt ttaatgacac agaaactaat
     3421 ctggcaggaa tgtacggaaa agttcgcaaa gctgctagtt caattgatca gtttagtatt
     3481 cgcctgggaa tttttctccg aagatacccc atagcgcgag tttttgtaat tatatatatg
     3541 gctttgcttc acctctgggt catgattgtt ctgttgactt acacaccaga aatgcaccac
     3601 gaccaaccat atggcaaatg aggatcgcgg ccgcatagat aactgatcca gtgtgctgga
     3661 attaattcgc tgtctgcgag ggccagctgt tggggtgagt actccctctc aaaagcgggc
     3721 atgacttctg cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg
     3781 cccgcggtga tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg
     3841 ttgtcaagct tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca
     3901 tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcag gtcgagcatg
     3961 catctagggc ggccaattcc gcccctctcc cccccccccc tctccctccc ccccccctaa
     4021 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     4081 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     4141 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     4201 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     4261 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     4321 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     4381 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     4441 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     4501 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     4561 acgatgataa gcttgccaca acccgggagg cgcgccatga agtgcctttt gtacttagcc
     4621 tttttattca ttggggtgaa ttgcaagttc accatagttt ttccacacaa ccaaaaagga
     4681 aactggaaaa atgttccttc taattaccat tattgcccgt caagctcaga tttaaattgg
     4741 cataatgact taataggcac agccttacaa gtcaaaatgc ccaagagtca caaggctatt
     4801 caagcagacg gttggatgtg tcatgcttcc aaatgggtca ctacttgtga tttccgctgg
     4861 tatggaccga agtatataac acattccatc cgatccttca ctccatctgt agaacaatgc
     4921 aaggaaagca ttgaacaaac gaaacaagga acttggctga atccaggctt ccctcctcaa
     4981 agttgtggat atgcaactgt gacggatgcc gaagcagtga ttgtccaggt gactcctcac
     5041 catgtgctgg ttgatgaata cacaggagaa tgggttgatt cacagttcat caacggaaaa
     5101 tgcagcaatt acatatgccc cactgtccat aactctacaa cctggcattc tgactataag
     5161 gtcaaagggc tatgtgattc taacctcatt tccatggaca tcaccttctt ctcagaggac
     5221 ggagagctat catccctggg aaaggagggc acagggttca gaagtaacta ctttgcttat
     5281 gaaactggag gcaaggcctg caaaatgcaa tactgcaagc attggggagt cagactccca
     5341 tcaggtgtct ggttcgagat ggctgataag gatctctttg ctgcagccag attccctgaa
     5401 tgcccagaag ggtcaagtat ctctgctcca tctcagacct cagtggatgt aagtctaatt
     5461 caggacgttg agaggatctt ggattattcc ctctgccaag aaacctggag caaaatcaga
     5521 gcgggtcttc caatctctcc agtggatctc agctatcttg ctcctaaaaa cccaggaacc
     5581 ggtcctgctt tcaccataat caatggtacc ctaaaatact ttgagaccag atacatcaga
     5641 gtcgatattg ctgctccaat cctctcaaga atggtcggaa tgatcagtgg aactaccaca
     5701 gaaagggaac tgtgggatga ctgggcacca tatgaagacg tggaaattgg acccaatgga
     5761 gttctgagga ccagttcagg atataagttt cctttataca tgattggaca tggtatgttg
     5821 gactccgatc ttcatcttag ctcaaaggct caggtgttcg aacatcctca cattcaagac
     5881 gctgcttcgc aacttcctga tgatgagagt ttattttttg gtgatactgg gctatccaaa
     5941 aatccaatcg agcttgtaga aggttggttc agtagttgga aaagctctat tgcctctttt
     6001 ttctttatca tagggttaat cattggacta ttcttggttc tccgagttgg tatccatctt
     6061 tgcattaaat taaagcacac caagaaaaga cagatttata cagacataga gatgaaccga
     6121 cttggaaagc tgaattccat ggacgagaag accactggtt ggcgaggtgg acacgttgtt
     6181 gaaggactgg ctggggaact tgaacaactt cgtgcacgac tggagcatca cccacaaggt
     6241 caacgtgaac cacctgcagg tatggtgagc aaaggcgagg agctgttcac cggggtggtg
     6301 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     6361 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     6421 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     6481 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     6541 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     6601 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     6661 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     6721 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     6781 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     6841 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     6901 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     6961 atggacgagc tgtacaaggg ccggccttaa ggcctcgagg gccaattaat taactctaga
     7021 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     7081 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     7141 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7201 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgtc
     7261 gagtgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7321 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7381 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7441 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7501 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7561 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7621 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7681 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7741 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7801 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7861 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7921 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7981 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8041 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8101 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8161 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8221 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8281 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8341 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8401 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8461 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8521 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8581 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8641 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8701 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8761 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8821 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8881 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8941 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9001 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9061 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9121 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9181 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9241 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9301 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9361 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9421 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9481 gcacatttcc ccgaaaagtg ccacctga
//
