LOCUS       sequence-322464-        9974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation Lentiviral vector with bi-cistronic expression of 
            dtomato and luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   Fujiwara T, Yakoub MA, Chandler A, Christ AB, Yang G, Ouerfelli O, 
            Rajasekhar VK, Yoshida A, Kondo H, Hata T, Tazawa H, Dogan Y, Moore 
            MAS, Fujiwara T, Ozaki T, Purdue E, Healey JH
  TITLE     CSF-1/CSF-1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms 
            Tumor-Associated Macrophages and Stimulates T-Cell Infiltration in 
            the Sarcoma Microenvironment.
  JOURNAL   Mol Cancer Ther. 2021 Jun 4. pii: 1535-7163.MCT-20-0591. doi: 
            10.1158/1535-7163.MCT-20-0591.
  PUBMED    34088832
REFERENCE   2  (bases 1 to 9974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Ther. 2021 Jun 4. pii: 1535-7163.MCT-20-0591. doi: 
            10.1158/1535-7163.MCT-20-0591."
COMMENT     Sequence Label: pUltra-Chili-Luc
FEATURES             Location/Qualifiers
     source          1..9974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    88..109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(124..129,130..147,148..154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    162..178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     167..189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     186..202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     186..202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     221..241
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        223..241
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        269..495
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             496..676
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    723..848
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1341..1574
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1759..1803
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2101..2218
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2283..3494
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3331..3350
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3537..3546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3543..4244
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(3676..3694)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3944..3963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4138..4161
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             4254..4310
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4317..5969
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4383..4401)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             5985..6038
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    6082..6670
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6135..6155)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6553..6564)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6761..6994
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7066..7187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7103..7122)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7157..7176
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7227..7362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7382..7401)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7383..7401)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7411..7428)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7411..7427)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7420..7442)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7569..8024
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7656..7675)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7866..7887
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8050..8154
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8155..8223,8224..9015)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8373..8392)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9186..9774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9675..9694
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9928..9945
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      121 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      181 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
      241 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
      301 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
      361 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
      421 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
      481 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
      541 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      601 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      661 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
      721 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
      781 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
      841 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
      901 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
      961 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     1021 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     1081 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     1141 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     1201 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     1261 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     1321 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     1381 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     1441 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     1501 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     1561 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     1621 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     1681 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     1741 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     1801 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     1861 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     1921 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     1981 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     2041 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
     2101 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     2161 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     2221 ggtttattac agggacagca gagatccagt ttggttaatt aacccgtgtc ggctccagat
     2281 ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc
     2341 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac
     2401 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
     2461 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
     2521 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
     2581 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
     2641 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg
     2701 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
     2761 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
     2821 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt
     2881 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc
     2941 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
     3001 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
     3061 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
     3121 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
     3181 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
     3241 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
     3301 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     3361 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     3421 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     3481 cttttttgtt agacgaagct tgggctgcag gtccgatcca ccggtggatc gatcccgcca
     3541 ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg
     3601 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     3661 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     3721 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
     3781 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     3841 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
     3901 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
     3961 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     4021 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     4081 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
     4141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     4201 gccgccacca cctgttcctg tacggcatgg acgagctgta caagggatct ggagcaacaa
     4261 acttctcact actcaaacaa gcaggtgacg tggaggagaa tcccgggcct tctagtatgg
     4321 aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg
     4381 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
     4441 ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc
     4501 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
     4561 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
     4621 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg ggcatttcgc
     4681 agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa
     4741 agctcccaat catccaaaaa attattatca tggattctaa aacggattac cagggatttc
     4801 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg
     4861 tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc tctggatcta
     4921 ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg
     4981 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc
     5041 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag
     5101 tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag gattacaaga
     5161 ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc actctgattg
     5221 acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg
     5281 aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa ggatatgggc
     5341 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg
     5401 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc
     5461 tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg tccggttatg
     5521 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag
     5581 acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg aagtctctga
     5641 ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg ctccaacacc
     5701 ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg
     5761 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg
     5821 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag
     5881 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg
     5941 ccaagaaggg cggaaagatc gccgtgtaaa gatccggctc cggcgagggc aggggaagtc
     6001 tactaacatg cggggacgtg gaggaaaatc ccggcccagc tagcgtgtga tcagaattcg
     6061 ttccggagtc gtcgactcga caatcaacct ctggattaca aaatttgtga aagattgact
     6121 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6181 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6241 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6301 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6361 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6421 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     6481 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6541 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6601 ctgcggcctc ttccgcatct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6661 gcctccccgc ctggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
     6721 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct acgtaactcc
     6781 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     6841 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6901 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6961 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7021 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     7081 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7141 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     7201 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7261 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7321 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     7381 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     7441 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     7501 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     7561 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     7621 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     7681 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     7741 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     7801 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7861 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     7921 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7981 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     8041 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8101 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     8161 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8221 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8281 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8341 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8401 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8461 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8521 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8581 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8641 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8701 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8761 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8821 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8881 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8941 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9001 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9061 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9121 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9181 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9241 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9301 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9361 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9421 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9481 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9541 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9601 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9661 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9721 tcacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9781 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9841 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9901 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9961 ccaatacgca aacc
//
