LOCUS       sequence-322467-        5192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase-based reporter for VEGF promoter region (-1000 to -1 bp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Wood LW, Cox NI, Phelps CA, Lai SC, Poddar A, Talbot C Jr, Mu D
  TITLE     Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of 
            Secretome.
  JOURNAL   Sci Rep. 2016 Feb 25;6:19857. doi: 10.1038/srep19857.
  PUBMED    26912193
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-25"; volume: "6:19857. doi"; pages: " 
            10.1038/srep19857"
COMMENT     Sequence Label: pGL4.10-VEGFprom(-1000 to -1)
FEATURES             Location/Qualifiers
     source          1..5192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1044..1053
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1050..2702
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2746..2867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2757..2776)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2811..2830
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3115..3132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3286..3874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3366..3385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4074..4865,4866..4934))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5039..5087
                     /note="synthetic polyadenylation signal"
     misc_feature    5101..5192
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5141..5160
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtaccc cagtcccaaa tatgtagctg tttgggaggt cagaaatagg
       61 gggtccagga gcaaactccc cccaccccct ttccaaagcc cattccctct ttagccagag
      121 ccggggtgtg cagacggcag tcactagggg gcgctcggcc accacaggga agctgggtga
      181 atggagcgag cagcgtcttc gagagtgagg acgtgtgtgt ctgtgtgggt gagtgagtgt
      241 gtgcgtgtgg ggttgagggt gttggagcgg ggagaaggcc aggggtcact ccaggattcc
      301 aatagatctg tgtgtccctc tccccacccg tccctgtccg gctctccgcc ttcccctgcc
      361 cccttcaata ttcctagcaa agagggaacg gctctcaggc cctgtccgca cgtaacctca
      421 ctttcctgct ccctcctcgc caatgccccg cgggcgcgtg tctctggaca gagtttccgg
      481 gggcggatgg gtaattttca ggctgtgaac cttggtgggg gtcgagcttc cccttcattg
      541 cggcgggctg cgggccaggc ttcactgagc gtccgcagag cccgggcccg agccgcgtgt
      601 ggaagggctg aggctcgcct gtccccgccc cccggggcgg gccgggggcg gggtcccggc
      661 ggggcggagc catgcgcccc cccctttttt ttttaaaagt cggctggtag cggggaggat
      721 cgcggaggct tggggcagcc gggtagctcg gaggtcgtgg cgctgggggc tagcaccagc
      781 gctctgtcgg gaggcgcagc ggttaggtgg accggtcagc ggactcaccg gccagggcgc
      841 tcggtgctgg aatttgatat tcattgatcc gggttttatc cctcttcttt tttcttaaac
      901 attttttttt aaaactgtat tgtttctcgt tttaatttat ttttgcttgc cattccccac
      961 ttgaatcggg ccgacggctt ggggagattg ctctacttcc ccaaatcact gtggaagctt
     1021 ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt aagaagggcc
     1081 cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga
     1141 agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca
     1201 ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg
     1261 ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc
     1321 ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg
     1381 agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga
     1441 aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca
     1501 tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc
     1561 atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa
     1621 ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac
     1681 cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga
     1741 tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca
     1801 ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg
     1861 agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac
     1921 tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg
     1981 agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct
     2041 tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga
     2101 tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg
     2161 ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt
     2221 gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc
     2281 tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc
     2341 acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc
     2401 cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg
     2461 gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta
     2521 aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga
     2581 agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg
     2641 acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtgt
     2701 aataattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg
     2761 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2821 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     2881 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     2941 acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt gagagccttc
     3001 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     3061 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
     3121 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3181 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3241 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3481 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3721 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3961 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4021 atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag
     4081 tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc
     4141 tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca
     4201 gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca
     4261 gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg
     4321 agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg
     4381 gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct
     4441 ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc
     4501 tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta
     4561 atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc
     4621 ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc
     4681 ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc
     4741 gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg
     4801 atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg
     4861 gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa
     4921 tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag
     4981 tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa
     5041 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact
     5101 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
     5161 cagtgcaagt gcaggtgcca gaacatttct ct
//
