LOCUS       sequence-322470-       11344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11344)
  AUTHORS   Kim J, Kato M, Beachy PA
  TITLE     Gli2 trafficking links Hedgehog-dependent activation of Smoothened 
            in the primary cilium to transcriptional activation in the nucleus.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21666-71. Epub 2009 
            Dec 8.
  PUBMED    19996169
REFERENCE   2  (bases 1 to 11344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2009-12-22"; volume: "106(51)"; pages: 
            "21666-71. Epub 2009 Dec 8"
COMMENT     Sequence Label: pCEFLmGFP-Gli2
FEATURES             Location/Qualifiers
     source          1..11344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          213..1155
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1112..1132
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(1192..1194,1195..1197,1198..1908)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1237..1258)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1498..1517)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1845..1866
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(6581..6599)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6582..6599)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6619..6636)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6625..6849
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6895..7323
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6982..7001)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7192..7213
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7332..7352)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7337..7666
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7517..7652
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7733..8527
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7787..7806)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8397..8416
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8701..8822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8738..8757)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8792..8811
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8871..8887)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8871..8887)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8884..8906)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8895..8911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8919..8925,8926..8943,8944..8949))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8964..8985
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9102..9119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9273..9861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9353..9372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10032..10823,10824..10892))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10655..10674
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10893..10997)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11076..11095)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(11326..11344,1..1164)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
       61 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      121 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      181 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
      241 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
      301 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
      361 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
      421 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
      481 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
      541 cgggccaaga tcgatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      601 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      661 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      721 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      781 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
      841 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
      901 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
      961 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     1021 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     1081 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     1141 tttcttccat ttcaggtgtc gtgaggaatt ggcgaagctt gtcgacccac catggtgagc
     1201 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1261 aacggccaca agttcagcgt gtctggcgag ggcgagggcg atgccaccta cggcaagctg
     1321 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1381 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     1441 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1501 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1561 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1621 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     1681 gcgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1741 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1801 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1861 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagac tagtgagact
     1921 tctgccccag cccctgcact ggagaagaaa gaagccaaga gtggtctctt ggaggacagc
     1981 agcttccccg acccagggaa aaaggcctgt cctctggcgg tggccgcagc tgtagccgcc
     2041 cacggagttc ctcagcagct cctgccggct ttccacgcgc ctttgccgat tgacatgaga
     2101 caccaggagg gaaggtacca ttatgaccct cactctgtcc acagtgtaca cgggcctccc
     2161 accctaagtg gcagccctgt catctcagat atctccttga tacgactttc tccacaccct
     2221 gctggccctg gagagtcacc cttcagcgcc caccacccct acgtgaaccc ccatatggag
     2281 cactacctcc ggtctgtgca cagcagcccc acactctcaa tgatctctgc cgccaggggc
     2341 ctcagccctg ctgatgtggc ccacgaacat ctgaaagaga ggggactctt tagcctcgca
     2401 gccccaggca ccaacccttc agactattac caccagatga ccctcatggc aagccacccc
     2461 accccttatg gggaccttct aatgcagagc gggggtgctg ctagcgcacc ccatctccat
     2521 gactacctca accctgtgga tgcatcacga ttctctagtc cacgtgtgac cccacgactg
     2581 agccgcaagc gggctctgtc catctccccg ctctcagatg ccagcctcga cctacaacgc
     2641 atgattcgga cctctcccaa ctcgctggta gcttacatca acaactccag gagcagctca
     2701 gcagccagtg gctcttatgg acatctgtct gctggtgccc tcagcccagc cttcactttt
     2761 ccccacccca tcaatcctgt ggcctaccag cagatcttga gccagcagcg gggcctgggc
     2821 tcagcctttg gacacacacc acccctgatc cagccttcac ccaccttctt ggcccagcag
     2881 cccatgactc tcacctccat cagcaccatg cctacccaac tcagcagcag tagcagcaac
     2941 tgtctaaatg atgccaacca gaacaagcag aacagcgagt cagctgtgag cagcaccgtg
     3001 aaccccatca ccattcataa gcggagcaag gtcaagactg aggctgaggg cctgcgtcca
     3061 gcatccccgc ttggactgac acaggagcag ctggccgatc tcaaggaaga cctggacagg
     3121 gatgactgta agcaggaggc cgaggtggtc atctacgaga ccaactgcca ctgggcagac
     3181 tgcaccaagg agtatgacac acaggagcag ctggtgcatc atatcaacaa tgaacacatc
     3241 cacggggaga agaaggagtt cgtgtgccgc tggcaggcct gcacgagaga gcagaagccc
     3301 ttcaaggccc agtacatgct ggttgttcac atgcgcagac acacgggtga gaagccacac
     3361 aagtgcacgt tcgaaggctg ttccaaggcc tactctcgcc tggagaacct gaagacacac
     3421 ctgcgttcac acacaggaga gaagccatat gtgtgtgaac acgaaggctg taacaaagcc
     3481 ttctccaatg cctcagaccg cgccaagcac cagaaccgca ctcactccaa tgagaaaccc
     3541 tacatctgca agatcccagg ctgcaccaag aggtacacag accccagctc actccgcaag
     3601 catgtgaaga ctgtccatgg gccagacgcc catgtcacca agaaacagcg taatgatgtg
     3661 catgtccgtg ctccactgct caaggagaat ggggataatg aggccagcgc cgagccaggt
     3721 ggccggggac ctgaggagag tgtggaggcc agtagcacca gccacactgt ggaggactgc
     3781 ctacatatca aagccatcaa gacagagagc tccgggcttt gtcagtccag ccccggggcc
     3841 cagtcatcct gcagcagcga gccctctccc ctgggcagtg cccccaacaa tgacagtggc
     3901 atggagatgc cggggacagg gcctgggagt ctgggagacc tgacagcact ggctgacacg
     3961 tgtccaggag ctgacacctc agccctggct gcaccctcca ctggtggcct gcagctgcgc
     4021 aaacacatga gcaccgtgca tcgctttgag cagctgaaga gagagaagct caagtcactg
     4081 aaggattcct gctcgtgggc cggcccagct ccacacaccc gcaacaccaa gctgcctccc
     4141 cttccagtca atggttctgt cctggaaaac ttcaacaata cagggggcgg tggaccggca
     4201 ggactgctgc ccagccagcg gctaccagag ctgaccgaag tgacgatgct gagccagctg
     4261 caggaacgaa gagacagctc caccagcacc atgagctcgg cctacactgt gagccgccgc
     4321 tcctctggca tctccccata cttctctagc cgtcgctcca gcgaggcttc gcctctcggt
     4381 ggcctacgcc cgcacaacgc cagctcagca gactcctatg accccatctc cacagatgcc
     4441 tctcggcgct ccagtgaagc cagccagtgc agtggcggtg gcccagagct gctcaacctc
     4501 acacctgcgc agcagtacaa cctgcgtgcc aagtacgcag cggccacagg tggaccaccg
     4561 cccacgccac tgccgggcct cgatcgtgta agccttcgta cccgcctggc cttgctggat
     4621 gctcctgagc gtgcacttcc tggtgcctgc ccacatccac tggggccacg gcgtggcagc
     4681 gatgggccta cctatagcca tggtcatggc catggctacg caggtgcggc tccagcattc
     4741 ccccacgagg ggccaaacag cagcacacgg cgggccagcg accctgtgcg gcgccctgac
     4801 ccccttattc tgcctcgagt gcaacgtttc cacagtaccc acaacatgaa tccaggttca
     4861 ctgccaccct gcgctgatcg gcgtggcctg cacgtacaga gccaccccag cgtagacagc
     4921 aacctgaccc gcaacgccta ctctcccaga ccccctagca tcaatgagaa cgtggtgatg
     4981 gaggccgtgg ctgctggggt agacggccca gggctagagt gcgacctggg gctggtggag
     5041 gatgagctgg tgctgccaga tgatgtggta cagtacatca aggctcacac cggtggtacc
     5101 ttggatgacg gcattcggca ggggtatccc acagaaggta ctggcttccc cgagaactct
     5161 aagctgccca gtcctgggct acaaggccac cgcaggctag cagctgccga ctccaacatg
     5221 ggtccttctg ctcctggact cgggggctgc cagctgagct acagcccctc ctccaacctc
     5281 aacaagagca acatgcctgt gcagtggaat gaggtgagtt ctggcaccgt ggatgccctg
     5341 cctacccagg tgaagccacc tcctttccct cacagcaacc tggctgtggt ccaacagaag
     5401 ccagcctttg gccagtatcc aggatataat ccacaatccg tgcagagcag ctccggaggt
     5461 ctagacagca cccagccgca cctacagctt cgaggagccc cctctgcatc aagagggagc
     5521 tacacgcaac agcctcgaca gccagctgca ggcagtcagt gcctgggtat gagtgcggcc
     5581 atgagcccgc aggccagcta cagccaagcc cacccccagc tgagcccaaa cattgtcagc
     5641 ggatctctga accagttttc tccctcctgc agcaatatgg cagccaagcc cagccacctg
     5701 ggactccctc agcaaatgga agttgtcccc aatgccacca tcatgaatgg ccatcaacgg
     5761 gagcacgggg tccccaattc atccctggct gcggtgtcac aacctcaccc agtcctgagc
     5821 tatccccagc aggacagcta ccaacagggc tccaaccttc tgtcatccca tcagcctggc
     5881 ttcatggagt cccagcagaa cgcgggcttt ggtctcatgc agcctcggcc acccctggaa
     5941 cccaacacgg ccagccgtca ccgtggagta cgttctgggc aacagcagtt gtatgccagg
     6001 accactggcc aagccatggt cacatcagcc aaccaagaga cagcagaagc tatgcccaag
     6061 ggaccagcag ggaccatggt atccctagct cctcagccat ctcaggacac agggcgggca
     6121 caagatcaga acacgctata ctactatggc cagatccaca tgtatgaaca gaatggaggc
     6181 tgcccagccg tgcagcccca gccgccacaa ccacaagctt gctcagacag tatccagcct
     6241 gagcctttgc cttcaccggg agtcaaccag gtgtctagca ccgtggactc ccagctcctg
     6301 gagccccccc agattgactt tgatgccatc atggatgatg gtgatcactc gagtttgttt
     6361 tctggtgcac tgagcccaac ccttctccac aatctctccc agaattcctc acgcctcacc
     6421 acaccccgga attccttgac actgccctcc atccctgcgg gcatcagcaa catggccgtg
     6481 ggcgacatga gttccatgct caccagcctg gctgaagaga gcaagttttt aaacatgatg
     6541 acctaagcgg ccgctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg
     6601 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     6661 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6721 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     6781 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     6841 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     6901 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6961 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7021 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7081 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7141 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     7201 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7261 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7321 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     7381 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     7441 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     7501 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7561 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     7621 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     7681 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     7741 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     7801 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     7861 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     7921 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     7981 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     8041 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     8101 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     8161 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     8221 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     8281 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     8341 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     8401 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     8461 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     8521 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     8581 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     8641 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     8701 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8761 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8821 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     8881 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8941 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9001 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9061 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9121 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9181 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9241 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9301 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9361 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9421 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9481 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9541 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9601 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9661 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9721 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9781 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9841 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9901 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9961 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10021 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10081 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10141 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10201 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10261 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10321 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10381 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10441 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10501 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10561 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10621 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10681 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10741 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10801 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10861 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10921 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10981 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    11041 gatcgatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    11101 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    11161 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
    11221 aggcgttttg cgctgcttcg agctttgcaa agatggataa agttttaaac agagaggaat
    11281 ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
    11341 cagt
//
