LOCUS       sequence-322510-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids 
            and D614G mutation for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman 
            J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter 
            G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt 
            AG, Iafrate AJ, Balazs AB
  TITLE     COVID-19-neutralizing antibodies predict disease severity and 
            survival.
  JOURNAL   Cell. 2021 Jan 21;184(2):476-488.e11. doi: 
            10.1016/j.cell.2020.12.015. Epub 2020 Dec 15.
  PUBMED    33412089
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.015"; journalName: "Cell"; date: 
            "2021-01-21- 21"; volume: "184"; issue: "2"; pages: "476-488.e11"
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 D614G
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      252..388
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    540..611
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(616..662)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    680..728
                     /note="synthetic polyadenylation signal"
     enhancer        809..1112
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1114..1316
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1267..1287
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          1450..1925
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     misc_feature    5822..6410
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5875..5895)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6293..6304)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6463..6584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6474..6493)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6528..6547
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      6699..6745
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      6775..6911
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    6956..7027
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        7072..7268
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7119..7254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7282..8076
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7336..7355)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7946..7965
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8087..8311
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(8507..9095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8587..8606)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(9841..9909,9910..10462,1..239)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10059..10078)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
       61 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
      121 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
      181 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
      241 cgattctagg tgcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact
      301 cccacatatg ccagattcag caacggatac ggcttcccca acttgcccac ttccatacgt
      361 gtcctcctta ccagaaattt atccttaaga tcccgaatcg tttaaactcg actctggctc
      421 tatcgaatct ccgtcgtttc gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg
      481 gcatcgaatc tcgtcagcta tcgtcagctt acctttttgg cagcgatcgc ggctcccgac
      541 atcttggacc attagctcca caggtatctt cttccctcta gtggtcataa cagcagcttc
      601 agctacctct caattcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
      661 tatcaatcgt tgcgttacac acacaaaaaa ccaacacaca tccatcttcg atggatagcg
      721 attttattat ctaactgctg atcgagtgta gccagatcta gtaatcaatt acggggtcat
      781 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      841 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      901 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      961 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     1021 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1081 acatctacgt attagtcatc gctattacca tgctgatgcg gttttggcag tacatcaatg
     1141 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     1201 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     1261 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
     1321 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     1381 accgggaccg atccagcctc ccctcgaagc ttacatgtgg taccgagctc ggatcctgag
     1441 aacttcaggg tgagtctatg ggacccttga tgttttcttt ccccttcttt tctatggtta
     1501 agttcatgtc ataggaaggg gagaagtaac agggtacaca tattgaccaa atcagggtaa
     1561 ttttgcattt gtaattttaa aaaatgcttt cttcttttaa tatacttttt tgtttatctt
     1621 atttctaata ctttccctaa tctctttctt tcagggcaat aatgatacaa tgtatcatgc
     1681 ctctttgcac cattctaaag aataacagtg ataatttctg ggttaaggca atagcaatat
     1741 ttctgcatat aaatatttct gcatataaat tgtaactgat gtaagaggtt tcatattgct
     1801 aatagcagct acaatccagc taccattctg cttttatttt atggttggga taaggctgga
     1861 ttattctgag tccaagctag gcccttttgc taatcatgtt catacctctt atcttcctcc
     1921 cacagctcct gggcaacgtg ctggtctgtg tgctggccca tcactttggc aaaggcggcc
     1981 gccatgtttg tgttcctcgt tctcctgccc ctcgtttctt cccaatgcgt gaacctcaca
     2041 actagaacgc agctcccacc cgcttacacg aactctttta ctcgtggagt atactatccc
     2101 gataaagtct ttaggtccag tgtccttcat tctactcaag accttttcct gccattcttc
     2161 tcaaatgtca cctggttcca tgctatccac gtttctggca ccaacggaac taaacgattt
     2221 gataatcccg tcttgccatt taacgatggc gtatactttg cctcaacgga gaagtccaac
     2281 attatccggg gatggatatt tggcaccacc ctggactcca agacacagtc tctcctcatt
     2341 gtgaacaacg ccaccaatgt cgtcatcaaa gtctgtgaat ttcaattttg taacgatccc
     2401 tttctcgggg tttattatca taagaataac aagtcttgga tggagtcaga gttccgcgtt
     2461 tacagttccg ctaacaactg tacttttgag tacgtttccc aaccatttct catggatctt
     2521 gaagggaagc aaggcaactt caagaatctc cgcgaatttg tgtttaagaa tatagacggc
     2581 tacttcaaaa tttatagcaa gcataccccg ataaatctgg ttcgggatct ccctcaaggc
     2641 ttctccgcgt tggaacccct cgtggacttg ccgattggca tcaacatcac ccggttccaa
     2701 acccttcttg cccttcatcg aagctacctc acgcctggcg acagtagtag tggttggaca
     2761 gcgggcgcag cagcctacta tgtgggatat ttgcaaccac gcacctttct tctcaaatat
     2821 aacgaaaacg gaacaattac cgatgccgta gactgtgccc tcgatcccct gtccgaaact
     2881 aaatgcacac tcaagtcctt cacagttgag aaaggcatat accaaaccag taacttccgc
     2941 gtccaaccca ccgaatccat tgttcgattc cctaacatca ccaatttgtg tcctttcggt
     3001 gaagtattta atgccactcg atttgcatca gtttatgcct ggaatcgcaa gcgaatatca
     3061 aattgtgttg cagattatag tgtcctgtat aatagcgcat cattcagcac ttttaagtgt
     3121 tatggggtca gccctacgaa attgaatgac ttgtgtttta ccaacgttta cgcagattct
     3181 ttcgtcatac gcggcgacga agtccgacag attgcaccgg ggcaaaccgg aaagatagca
     3241 gactataatt ataagttgcc ggatgacttc accggctgcg ttatagcatg gaactccaat
     3301 aatctcgatt caaaagtcgg cggcaactat aattatcttt accgcctgtt tcgcaagtca
     3361 aatctgaagc cctttgaacg tgacatctcc accgaaattt atcaagctgg cagcacccca
     3421 tgtaatggag tggaagggtt taactgttat tttccccttc aatcatacgg atttcaacca
     3481 acaaatgggg tgggctatca accatatcgg gtggtagtct tgagctttga gctgctccac
     3541 gcaccggcta cagtttgcgg accgaagaaa agcactaacc tggtgaagaa taaatgtgtg
     3601 aacttcaact ttaatgggct caccggaaca ggggtgctga cagaatcaaa caagaaattt
     3661 ctcccttttc aacaatttgg tagagacatt gccgatacca cagatgccgt ccgcgatccg
     3721 caaacactgg aaatcctcga tataacaccc tgtagcttcg gcggggtcag cgtgataact
     3781 cccggaacga acacatccaa ccaagtggct gtcctgtatc aaggtgtcaa ctgtaccgaa
     3841 gtccctgtgg cgatacacgc cgaccaactg acacccacct ggagagtcta ctcaacaggt
     3901 tccaatgtct ttcaaactag agcgggttgt ctcattggcg ctgagcatgt caataattca
     3961 tacgaatgtg atattcctat tggggcagga atctgtgcgt cttatcaaac tcaaacgaac
     4021 tcaccacggc gcgctcgctc agtagccagc cagtccataa tcgcctatac catgagtctc
     4081 ggggctgaga actccgtggc ttattcaaac aattccatcg caatcccaac taactttacc
     4141 attagcgtta ccacggaaat cttgcccgtc agcatgacta agacgagcgt agactgtacg
     4201 atgtatatct gtggggattc caccgagtgt agcaacctct tgcttcaata cgggtctttc
     4261 tgtactcaac tcaaccgagc attgaccgga attgcggtcg aacaagacaa gaatacccaa
     4321 gaggtgttcg cacaagtaaa gcaaatatac aaaacgccgc caatcaaaga ttttggcggg
     4381 tttaatttta gccaaatact gccagaccca tctaagcctt caaaacgcag ctttatcgag
     4441 gatctgctgt tcaacaaagt tacgttggct gacgcgggtt tcattaagca gtatggggat
     4501 tgtttgggag acattgcggc ccgcgacctt atctgtgctc aaaaattcaa tgggttgaca
     4561 gttctgcccc ctctgctcac tgatgagatg attgcacagt atacatcagc attgttggca
     4621 ggaaccatta catccggctg gacttttggc gcgggtgcgg cccttcaaat acctttcgct
     4681 atgcaaatgg cttacagatt caatgggatt ggtgtcacgc aaaatgtatt gtatgaaaat
     4741 cagaagctga tcgctaatca atttaacagc gcgattggca agatccaaga ttcactgtca
     4801 agcacagcgt ccgccctcgg caaactccaa gatgttgtga atcaaaacgc ccaggccctg
     4861 aataccctcg tgaagcaact gagttctaac ttcggcgcaa tctccagcgt cctcaacgac
     4921 atattgtcca gacttgataa ggtggaagcc gaggtgcaaa ttgatcggct catcacaggc
     4981 aggctccagt cactccaaac ttatgtgaca caacagttga tccgggctgc tgagatacga
     5041 gcaagcgcca acctggccgc cactaaaatg tcagagtgcg tgctcgggca gtccaaacgg
     5101 gttgactttt gtggcaaggg ctatcacttg atgtccttcc cccaatccgc cccacatgga
     5161 gtggtctttc tccacgtcac ttatgtccct gctcaagaga agaactttac gacagctcca
     5221 gcaatctgtc acgatggcaa agcgcacttt cccagggaag gcgttttcgt cagtaatggc
     5281 acgcattggt ttgtgaccca aaggaatttc tatgaacccc aaatcataac cactgacaat
     5341 acatttgtct caggcaactg tgatgtggtc attgggatcg tgaacaacac ggtatatgat
     5401 cccctgcaac ccgagttgga tagcttcaaa gaggaattgg ataaatactt taagaatcat
     5461 acttcacccg atgttgatct cggggacata tcaggaataa atgcaagtgt cgtcaacatt
     5521 cagaaagaga ttgatcgcct taatgaggtc gccaagaact tgaatgaatc tttgatcgac
     5581 ttgcaagaac tgggcaaata cgaacaatat attaaatggc cctggtacat ttggttgggc
     5641 tttatcgccg gacttatagc tatcgttatg gtcacaatta tgttgtgctg tatgactagc
     5701 tgttgttcct gcttgaaagg atgttgcagt tgcggatctt gttgtaaata aaggatcctc
     5761 tagaagttgt ctcctcctgc actgactgac tgatacaatc gatttctgga tccgcaggcc
     5821 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5881 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5941 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6001 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6061 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6121 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6181 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     6241 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6301 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6361 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtctagct
     6421 tgactgactg agatacagcg taccttcagc tcacagacat gataagatac attgatgagt
     6481 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     6541 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     6601 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     6661 tctacaaatg tggtattggc ccatctctat cggtatcgta gcataacccc ttggggcctc
     6721 taaacgggtc ttgaggggtt ttttgtgccc ctcgggccgg attgctatct accggcattg
     6781 gcgcagaaaa aaatgcctga tgcgacgctg cgcgtcttat actcccacat atgccagatt
     6841 cagcaacgga tacggcttcc ccaacttgcc cacttccata cgtgtcctcc ttaccagaaa
     6901 tttatcctta aggtcgtcag ctatcctgca ggcgatctct cgatttcgat caagacattc
     6961 ctttaatggt cttttctgga caccactagg ggtcagaagt agttcatcaa actttcttcc
     7021 ctccctaatc tcattggtta ccttgggcta tcgaaactta attaagcgat ctgcatctca
     7081 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7141 gttccgccca ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg
     7201 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7261 tttgcaaagg aggtagccaa catgattgaa caagatggat tgcacgcagg ttctcccgcc
     7321 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     7381 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     7441 tccggtgccc tgaatgaact ccaggacgag gcagcgcggc tatcgtggct ggccacgacg
     7501 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     7561 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     7621 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     7681 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     7741 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     7801 ctcaaggcgc ggatgcccga cggcgaggat ctcgtcgtga cccacggcga tgcctgcttg
     7861 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     7921 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     7981 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     8041 atcgccttct atcgccttct tgacgagttc ttctagtatg taagccctgt gccttctagt
     8101 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     8161 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     8221 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     8281 aggcatgctg gggatgcggt gggctctatg gttaattaac cagtcaagtc agctacttgg
     8341 cgagatcgac ttgtctgggt ttcgactacg ctcagaattg cgtcagtcaa gttcgatctg
     8401 gtccttgcta ttgcacccgt tctccgatta cgagtttcat ttaaatcatg tgagcaaaag
     8461 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8521 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8581 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8641 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8701 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8761 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8821 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8881 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8941 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9001 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9061 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9121 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9181 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9241 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9301 cgatctgtct atttcgttca tccatagttg catttaaatt tccgaactct ccaaggccct
     9361 cgtcggaaaa tcttcaaacc tttcgtccga tccatcttgc aggctacctc tcgaacgaac
     9421 tatcgcaagt ctcttggccg gccttgcgcc ttggctattg cttggcagcg cctatcgcca
     9481 ggtattactc caatcccgaa tatccgagat cgggatcacc cgagagaagt tcaacctaca
     9541 tcctcaatcc cgatctatcc gagatccgag gaatatcgaa atcggggcgc gcctggtgta
     9601 ccgagaacga tcctctcagt gcgagtctcg acgatccata tcgttgcttg gcagtcagcc
     9661 agtcggaatc cagcttggga cccaggaagt ccaatcgtca gatattgtac tcaagcctgg
     9721 tcacggcagc gtaccgatct gtttaaacct agatattgat agtctgatcg gtcaacgtat
     9781 aatcgagtcc tagcttttgc aaacatctat caagagacag gatcagcagg aggctttcgc
     9841 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9901 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcg
     9961 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
    10021 gaagaacgct ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
    10081 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
    10141 gttgagtatt caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
    10201 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatt
    10261 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
    10321 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
    10381 cctgtagcaa tggcaacaac cttgcgtaaa ctattaactg gcgaactact tactctagct
    10441 tcccggcaac agttgataga ct
//
