LOCUS       sequence-322522-        8377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of membrane-localized mCherry driven by the ser-2promo3 
            promoter. For use as a PVD neuron marker in C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Feng Z, Zhao Y, Li T, Nie W, Yang X, Wang X, Wu J, Liao J, Zou Y
  TITLE     CATP-8/P5A ATPase Regulates ER Processing of the DMA-1 Receptor for 
            Dendritic Branching.
  JOURNAL   Cell Rep. 2020 Sep 8;32(10):108101. doi: 
            10.1016/j.celrep.2020.108101.
  PUBMED    32905774
REFERENCE   2  (bases 1 to 8377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-09-8"; volume: "32(10):108101. doi"; pages: " 
            10.1016/j.celrep.2020.108101"
COMMENT     Sequence Label: pSM_ser-2prom3p::myr-mCherry
FEATURES             Location/Qualifiers
     source          1..8377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5947..5969)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6069..6088
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6170..6188)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6256..6360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6361..6429,6430..7221)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6579..6598)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7392..7980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7881..7900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8134..8151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8268..8289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8304..8309,8310..8327,8328..8334)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8342..8358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8347..8369
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(8366..8377,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(8366..8377,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgtaaaag tttagtaaat taactgctaa agaatataaa
       61 gctaacacag caaaacatgt gaatgatcca acgataatca ccacccacaa catagctttg
      121 agttgattat ttgagttttt aaatcaaaat cgtcttttta aaacaggata aacccgacga
      181 aattcaaaaa attcccagaa agtgaaaaaa aatcaagcaa gtatgtgtct ttgatttttg
      241 tagttttttt ttacaaattt caaaagatct agtatcctaa attataccgt atattcccta
      301 ttagtatcgc ccccctcact cgacttatcg tttatatctt ggttgtcttt gatcatattt
      361 taaaaattca aacgacaaaa tctttttcaa atctatcgtt atgctctggt ttttgaagat
      421 atcaaaaatc ttctgaaacc gctgaaatat tagccgacaa agtcgattaa tggggggcca
      481 taataatagg gaatagtgtt tttcccatat tccctaattg ttttccaaat atcaattttc
      541 acagttttaa tttaattgcg cttactacat agttttaact aagttttaat attcgaaact
      601 agctgttaca aaaaggttat gaaattacat tttcagacga tttctgaatt tttaaacatt
      661 caaaaggtct tgctatattg tttgatccaa ataaacaaaa agcctgtaca agaaacatat
      721 cttatttcat cataaataat gttgttctat tttgaaataa ttacataatt aagttgaaaa
      781 attgtcagaa gccggaaaaa aacacgaatc gactttccca gcaagaatat cagaaaacct
      841 gttgagcaaa aaaaaacaat ctcgttgttg aaaaaaccag atagctggaa gaaattgaaa
      901 ataaaaagac aacacacata tatactaaaa acatgaggca aaagaactgt ctattgaaac
      961 acgaaacgag gagaaaaaaa aataaaaacg gccttgtgat tgtccagttg agttttgttt
     1021 cacacggttt ttacgatcaa tgcaaagaaa aaaaaaggat tcaataatgg tggaagaaat
     1081 attcgtggaa gtacaactat gtactacaac ttatgaattt gaatttcatt cccgaaaatt
     1141 tgtcggaaaa ttgtgtaaaa ggggtcatat agataataac cgatgtgggg ggagaaagga
     1201 tgaagaagtg gtgggaggca aaacaaagat gaatccgaaa aggatgcagc aattcagaac
     1261 agatgcatcc cggaaaatga aaacgaaggt catcgctatt tcttaccttt tcaccaaaat
     1321 ctttcttctt cactgatcca gggtcagaaa tttgaaattt tgaaggacaa cagtgttgcc
     1381 atagcaggaa ctgggggctg gggctggggg gagaaaattt atttctcacg tcgtttttct
     1441 caaccttctt cagtctccta gatatctcct agaaaccttt gagttatacg agtgaagaga
     1501 cggaaaagcc acagaataag ggaaaaatgt aagtaagagt ggtggagagc caaggaaatg
     1561 tgacaaatta ataacacgac ggcctacaac tcactttgtt ataataattt taatttgaaa
     1621 tcttctttct tgcaaaaaga gtactagtga aggacggatg aagaacgatg gaatacttga
     1681 aaatcttctc agatcttctt ctttcctcct cgaaattaga gttaatccaa aagataaaca
     1741 gttgtaagtg gtgtgtgaat cttcaagaga gccggagatg acagtctcag aggtgagtca
     1801 ctgttttttt ttgttggatt gtgctgggct tagctgctag ccggatgtaa gaatattgaa
     1861 aaaatgaata taaaaagttt aatgatataa accggtagcg agatgatata taagttcaga
     1921 tgtgttgata aatagggttt ttcgtttaaa tgtactgata attattgaaa attttaaatt
     1981 ttctagaaaa tcagttcaaa aaattttgat tttgtaataa caaaaaagtt cagtcaaacg
     2041 ttcaggcagt tgccatgagt ttagtaaagc tagacttttt gagaacattt tttgttgaaa
     2101 taattgtttt aattttaaaa tgtttagttc aaataactag aacatacata agtagaacat
     2161 aaaatttgaa taaaattgtc gtcttccaaa attgtaaaat tatattgcaa aaactgtgta
     2221 actcaaactg ctgatcaaaa tatttattga agtttaacag agtttaaaac acaaaattat
     2281 agtgaagaaa tgcacagaaa atgcatccaa actcaattat ttcctagaat gaagaatttt
     2341 aaaaatgatg ttataacaaa attcttttca attcaaaaac tagattgtat ttacaacgtc
     2401 ggaatgatta aaacacattt agaatataaa attcttaaaa taaattaaaa ttttgtggac
     2461 tggacttttt ggagacttgg cgaattatgg ttattccgaa acgctgtcga cttcaactgt
     2521 aggcgaaata cacgcgcgcg gaactcgtaa gatgtcggcg gccttatatt gggtggggtc
     2581 ccgatttttg cctttttccc gtgagtggac gagcaaaatt cggggcttct ttgggcgtac
     2641 acgccaagca gttttattat tttgagtgta tagctcttaa aaaactgtaa aaatgtgttt
     2701 gaaaaagata tgctatgaat tttgatcatt ttatccactg tactgaaact cgaacataaa
     2761 aaccaaaatt atacgaaaaa atcgtttgga gacggcgatt ctaacttttg gtgatgaaaa
     2821 acttatcgaa atgttacatt tcgaatcttt gatatcagaa aaaaagtcac tgatcatctt
     2881 ctgaaataaa ttaacaagaa agtattcctc tagtatcaaa atgtctctag tggctttctc
     2941 aaagacatta atccccatat ctcatagttc agacgaactt tccacagtaa acaatactag
     3001 acgttcagaa tatgaaattt aatcatgttt tatatgaaaa aagttgctcc attcaatttt
     3061 aatactcgac aaaaaaggaa ccaagttcct tagtgcaaaa attccaaaaa tctaaaacag
     3121 gttccactga gctttggcaa aatgattaga agagcatttc aatgctcttc taatatgttt
     3181 ctaggtgtta ttttttttct caattttttt ttgtaacttt cttgttttag caacaacaaa
     3241 aattatttca tttgaaatta cagaaacaga aattctcaaa atgctgtttc caaaaatcag
     3301 gtataatttg ttcacacaat taataatgtt tctagaaaat agttttattt tgacgcaact
     3361 aagaaacata aaaacatctt ttaatcagtc agttttaatc aggggcagat agctcagccg
     3421 gtagcgccgg ccgctagcaa tctggaggtc acgagttcaa gtcccgcctc accccctatg
     3481 ctcacccatc atctattagg aaatggagca atccccaact agattatcgg ccacagtccc
     3541 cggctaggac gtggcttaaa ttacagctca gtgggagcac caccaggcag tgtacctgaa
     3601 tcccagatcc gcagtacata gcacttgaag aacggatcgt cctttaatcc ttttttttta
     3661 aatctcgttt caaaattatt ttaaacccat ttttttcaaa ttgttacttg aattcagtgt
     3721 ttctgaattt attacagcca tatgtgtttt gtgatttctg atccatctcg attcggtttc
     3781 cacttctcat atccatttct caacccgttt tttttgtatt ctaccttttt cctaccaact
     3841 catgtcaatt tcgcacaaca taacaaatca caacgtctaa aagcgaagca aatgaatgta
     3901 aaagaaggtt atcaatcaga gtctcctcgg gctccttcct tttgaggttt gatgacgcga
     3961 aaaatcggaa gggccttgcg ttgtcctccg gccagtagca attcatttcg tagacgtttt
     4021 tttcccctgc tacattctga cgacaataat attatttttg ttggctaaat ttactatagc
     4081 taattattca ttttggcata tttttgtgac atcacaacac ataatgggat ccccgggatt
     4141 ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa cattttcagg
     4201 aggacccttg gctagcgggc cggccatggg atcatgtatt ggaaaagtct caaagggtga
     4261 agaagataac atggcaatta ttaaagagtt tatgcgtttc aaggtgcata tggagggatc
     4321 tgtcaatggg catgagtttg aaattgaagg tgaaggagaa ggccgaccat atgagggaac
     4381 acaaaccgca aaactaaagg taagtttaaa catatatata ctaactaacc ctgattattt
     4441 aaattttcag gtaactaaag gcggaccatt accattcgcc tgggacatcc tctctccaca
     4501 gttcatgtat ggaagtaaag cttatgttaa acatccggca gatataccag attatttgaa
     4561 actttcattc ccggagggtt ttaagtggga acgcgtaatg aattttgaag acggaggagt
     4621 tgttacagtg acgcaagact caaggtaagt ttaaacagtt cggtactaac taaccataca
     4681 tatttaaatt ttcagcctcc aagatggaga atttatttat aaagtcaaac ttcgaggaac
     4741 gaatttcccc tcggatggac ctgttatgca gaagaagact atgggatggg aagcttcaag
     4801 tgaaagaatg taccctgaag acggtgctct taagggagag attaaacaac gtcttaaatt
     4861 gaaagatgga ggacattacg atgctgaggt aagtttaaac atgattttac taactaacta
     4921 atctgattta aattttcagg tgaagacaac ttacaaagcc aaaaaaccag ttcagctgcc
     4981 aggagcgtac aatgttaata ttaaactgga tatcacctcc cacaacgagg attacactat
     5041 cgttgagcaa tatgaaagag ctgaagggcg gcactcgaca ggtggcatgg atgaattgta
     5101 taagtaaggt accatggtat tgatatctga gctccgcatc ggccgctgtc atcagatcgc
     5161 catctcgcgc ccgtgcctct gacttctaag tccaattact cttcaacatc cctacatgct
     5221 ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaacttc tcttaatttc
     5281 tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata
     5341 gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat
     5401 tctatcccaa aatctacaca atgttctgtg tacacttctt atgtttttta cttctgataa
     5461 atttttttga aacatcatag aaaaaaccgc acacaaaata ccttatcata tgttacgttt
     5521 cagtttatga ccgcaatttt tatttcttcg cacgtctggg cctctcatga cgtcaaatca
     5581 tgctcatcgt gaaaaagttt tggagtattt ttggaatttt tcaatcaagt gaaagtttat
     5641 gaaattaatt ttcctgcttt tgctttttgg ggtttcccct attgtttgtc aagatttcga
     5701 ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg
     5761 aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg
     5821 agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat
     5881 aactgtttct actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     5941 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     6001 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     6061 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     6121 gcacagatgc gtaaggagaa aataccgcat caggcggcct taattaaggg cctcgtgata
     6181 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     6241 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6301 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6361 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6421 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6481 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6541 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6601 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6661 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6721 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6781 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6841 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6901 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6961 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7021 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7081 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7141 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7201 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7261 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7321 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7381 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7441 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7501 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     7561 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7621 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7681 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7741 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7801 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7861 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7921 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7981 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8041 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8101 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8161 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     8221 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     8281 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     8341 attgtgagcg gataacaatt tcacacagga aacagct
//
