LOCUS       sequence-322546-       11453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of GFP-BRCA1 siRNA resistant construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 11453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pDEST-FRT/T0-GFP-BRCA1
FEATURES             Location/Qualifiers
     source          1..11453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(990..992,993..995,996..1706)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(990..992,993..995,996..1706)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1035..1056)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1296..1315)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1643..1664
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1716..1740
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7339..7363)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7405..7422)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7411..7635
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7768..7787)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7919..7966
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7974..8996
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9126..9247
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9163..9182)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9217..9236
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9296..9312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9296..9312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9309..9331)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9320..9336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9344..9350,9351..9368,9369..9374))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9389..9410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9527..9544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9698..10286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9778..9797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10457..11248,11249..11317))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11080..11099
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11318..11422)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tggtgagcaa gggcgaggag ctgttcaccg
     1021 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1081 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1141 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     1201 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1261 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1321 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1381 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1441 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1501 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1561 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1621 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1681 ctctcggcat ggacgagctg tacaagggcg cgcccacaag tttgtacaaa aaagcaggct
     1741 tcaccatgga tttatctgct cttcgcgttg aagaagtaca aaatgtcatt aatgctatgc
     1801 agaaaatctt agagtgtccc atctgtctgg agttgatcaa ggaacctgtc tccacaaagt
     1861 gtgaccacat attttgcaaa ttttgcatgc tgaaacttct caaccagaag aaagggcctt
     1921 cacagtgtcc tttatgtaag aatgatataa ccaaaaggag cctacaagaa agtacgagat
     1981 ttagtcaact tgttgaagag ctattgaaaa tcatttgtgc ttttcagctt gacacaggtt
     2041 tggagtatgc aaacagctat aattttgcaa aaaaggaaaa taactctcct gaacatctaa
     2101 aagatgaagt ttctatcatc caaagtatgg gctacagaaa ccgtgccaaa agacttctac
     2161 agagtgaacc cgaaaatcct tccttgcagg aaaccagtct cagtgtccaa ctctctaacc
     2221 ttggaactgt gagaactctg aggacaaagc agcggataca acctcaaaag acgtctgtct
     2281 acattgaatt gggatctgat tcttctgaag ataccgttaa taaggcaact tattgcagtg
     2341 tgggagatca agaattgtta caaatcaccc ctcaaggaac cagggatgaa atcagtttgg
     2401 attctgcaaa aaaggctgct tgtgaatttt ctgagacgga tgtaacaaat actgaacatc
     2461 atcaacccag taataatgat ttgaacacca ctgagaagcg tgcagctgag aggcatccag
     2521 aaaagtatca gggtagttct gtttcaaact tgcatgtgga gccatgtggc acaaatactc
     2581 atgccagctc attacagcat gagaacagca gtttattact cactaaagac agaatgaatg
     2641 tagaaaaggc tgaattctgt aataaaagca aacagcctgg cttagcaagg agccaacata
     2701 acagatgggc tggaagtaag gaaacatgta atgataggcg gactcccagc acagaaaaaa
     2761 aggtagatct gaatgctgat cccctgtgtg agagaaaaga atggaataag cagaaactgc
     2821 catgctcaga gaatcctaga gatactgaag atgttccttg gataacacta aatagcagca
     2881 ttcagaaagt taatgagtgg ttttccagaa gtgatgaact gttaggttct gatgactcac
     2941 atgatgggga gtctgaatca aatgccaaag tagctgatgt attggacgtt ctaaatgagg
     3001 tagatgaata ttctggttct tcagagaaaa tagacttact ggccagtgat cctcatgagg
     3061 ctttaatatg taaaagtgaa agagttcact ccaaatcagt agagagtaat attgaagaca
     3121 aaatatttgg gaaaacctat cggaagaagg caagcctccc caacttaagc catgtaactg
     3181 aaaatctaat tataggagca tttgttactg agccacagat aatacaagag cgtcccctca
     3241 caaataaatt aaagcgtaaa aggagaccta catcaggcct tcatcctgag gattttatca
     3301 agaaagcaga tttggcagtt caaaagactc ctgaaatgat aaatcaggga actaaccaaa
     3361 cggagcagaa tggtcaagtg atgaatatta ctaatagtgg tcatgagaat aaaacaaaag
     3421 gtgattctat tcagaatgag aaaaatccta acccaataga atcactcgaa aaagaatctg
     3481 ctttcaaaac gaaagctgaa cctataagca gcagtataag caatatggaa ctcgaattaa
     3541 atatccacaa ttcaaaagca cctaaaaaga ataggctgag gaggaagtct tctaccaggc
     3601 atattcatgc gcttgaacta gtagtcagta gaaatctaag cccacctaat tgtactgaat
     3661 tgcaaattga tagttgttct agcagtgaag agataaagaa aaaaaagtac aaccaaatgc
     3721 cagtcaggca cagcagaaac ctacaactca tggaaggtaa agaacctgca actggagcca
     3781 agaagagtaa caagccaaat gaacagacaa gtaaaagaca tgacagtgat actttcccag
     3841 agctgaagtt aacaaatgca cctggttctt ttactaagtg ttcaaatacc agtgaactta
     3901 aagaatttgt caatcctagc cttccaagag aagaaaaaga agagaaacta gaaacagtta
     3961 aagtgtctaa taatgctgaa gaccccaaag atctcatgtt aagtggagaa agggttttgc
     4021 aaactgaaag atctgtagag agtagcagta tttcactggt acctggtact gattatggca
     4081 ctcaggaaag tatctcgtta ctggaagtta gcactctagg gaaggcaaaa acagaaccaa
     4141 ataaatgtgt gagtcagtgt gcagcatttg aaaaccccaa gggactaatt catggttgtt
     4201 ccaaagataa tagaaatgac acagaaggct ttaagtatcc attgggacat gaagttaacc
     4261 acagtcggga aacaagcata gaaatggaag aaagtgaact tgatgctcag tatttgcaga
     4321 atacattcaa ggtttcaaag cgccagtcat ttgctctgtt ttcaaatcca ggaaatgcag
     4381 aagaggaatg tgcaacattc tctgcccact ctgggtcctt aaagaaacaa agtccaaaag
     4441 tcacttttga atgtgaacaa aaggaagaaa atcaaggaaa gaatgagtct aatatcaagc
     4501 ctgtacagac agttaatatc actgcaggct ttcctgtggt tggtcagaaa gataagccag
     4561 ttgataatgc caaatgtagt atcaaaggag gctctaggtt ttgtctatca tctcagttca
     4621 gaggcaacga aactggactc attactccaa ataaacatgg acttttacaa aacccatatc
     4681 gtataccacc actttttccc atcaagtcat ttgttaaaac taaatgtaag aaaaatctgc
     4741 tagaggaaaa ctttgaggaa cattcaatgt cacctgaaag agaaatggga aatgagaaca
     4801 ttccaagtac agtgagcaca attagccgta ataacattag agaaaatgtt tttaaaggag
     4861 ccagctcaag caatattaat gaagtaggtt ccagtactaa tgaagtgggc tccagtatta
     4921 atgaaatagg ttccagtgat gaaaacattc aagcagaact aggtagaaac agagggccaa
     4981 aattgaatgc tatgcttaga ttaggggttt tgcaacctga ggtctataaa caaagtcttc
     5041 ctggaagtaa ttgtaagcat cctgaaataa aaaagcaaga atatgaagaa gtagttcaga
     5101 ctgttaatac agatttctct ccatatctga tttcagataa cttagaacag cctatgggaa
     5161 gtagtcatac atctcaggtt tgttctgaga cacctgatga cctgttagat gatggtgaaa
     5221 taaaggaaga tactagtttt gctgaaaatg acattaagga aagttctgct gtttttagca
     5281 aaagcgtcca gagaggagag cttagcagga gtcctagccc tttcacccat acacatttgg
     5341 ctcagggtta ccgaagaggg gccaagaaat tagagtcctc agaagagaac ttatctagtg
     5401 aggatgaaga gcttccctgc ttccaacact tgttatttgg taaagtaaac aatatacctt
     5461 ctcagtctac taggcatagc accgttgcta ccgagtgtct gtctaagaac acagaggaga
     5521 atttattatc attgaagaat agcttaaatg actgcagtaa ccaggtaata ttggcaaagg
     5581 catctcagga acatcacctt agtgaggaaa caaaatgttc tgctagcttg ttttcttcac
     5641 agtgcagtga attggaagac ttgactgcaa atacaaacac ccaggatcct ttcttgattg
     5701 gttcttccaa acaaatgagg catcagtctg aaagccaggg agttggtctg agtgacaagg
     5761 aattggtttc agatgatgaa gaaagaggaa cgggcttgga agaaaataat caagaagagc
     5821 aaagcatgga ttcaaactta ggtgaagcag catctgggtg tgagagtgaa acaagcgtct
     5881 ctgaagactg ctcagggcta tcctctcaga gtgacatttt aaccactcag cagagggata
     5941 ccatgcaaca taacctgata aagctccagc aggaaatggc tgaactagaa gctgtgttag
     6001 aacagcatgg gagccagcct tctaacagct accctagtat aatcagtgac tcttctgccc
     6061 ttgaggacct gcgaaatcca gaacaaagca catcagaaaa agcagtatta acttcacaga
     6121 aaagtagtga ataccctata agccagaatc cagaaggcct ttctgctgac aagtttgagg
     6181 tgtctgcaga tagttctacc agtaaaaata aagaaccagg agtggaaagg tcatcccctt
     6241 ctaaatgccc atcattagat gataggtggt acatgcacag ttgctctggg agtcttcaga
     6301 atagaaacta cccatctcaa gaggagctca ttaaggttgt tgatgtggag gagcaacagc
     6361 tggaagagtc tgggccacac gatttgacgg aaacatctta cttgccaagg caagatctag
     6421 agggaacccc ttacctggaa tctggaatca gcctcttctc tgatgaccct gaatctgatc
     6481 cttctgaaga cagagcccca gagtcagctc gtgttggcaa cataccatct tcaacctctg
     6541 cattgaaagt tccccaattg aaagttgcag aatctgccca gggtccagct gctgctcata
     6601 ctactgatac tgctgggtat aatgcaatgg aagaaagtgt gagcagggag aagccagaat
     6661 tgacagcttc aacagaaagg gtcaacaaaa gaatgtccat ggtggtgagt ggcctgaccc
     6721 cagaagaatt tatgctcgtg tacaagtttg ccagaaaaca ccacatcact ttaactaatc
     6781 taattactga agagactact catgttgtta tgaaaacaga tgctgagttt gtgtgtgaac
     6841 ggacactgaa atattttcta ggaattgcgg gaggaaaatg ggtagttagc tatttctggg
     6901 tgacccagtc tattaaagaa agaaaaatgc tgaatgagca tgattttgaa gtcagaggag
     6961 atgtggtcaa tggaagaaac caccaaggtc caaagcgagc aagagaatcc caggacagaa
     7021 agatcttcag ggggctagaa atctgttgct atgggccctt caccaacatg cccacagatc
     7081 aactggaatg gatggtacag ctgtgtggtg cttctgtggt gaaggagctt tcatcattca
     7141 cccttggcac aggtgtccac ccaattgtgg ttgtgcagcc agatgcctgg acagaggaca
     7201 atggcttcca tgcaattggg cagatgtgtg aggcacctgt ggtgacccga gagtgggtgt
     7261 tggacagtgt agcactctac cagtgccagg agctggacac ctacctgata ccccagatcc
     7321 cccacagcca ctactgagac ccagctttct tgtacaaagt ggtgtgactc gagtctagag
     7381 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7441 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7501 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7561 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     7621 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     7681 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7741 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7801 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7861 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtacctaga
     7921 agttcctatt ccgaagttcc tattctctag aaagtatagg aacttccttg gccaaaaagc
     7981 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     8041 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     8101 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     8161 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggagttca
     8221 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     8281 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     8341 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     8401 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     8461 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     8521 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     8581 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     8641 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     8701 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     8761 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     8821 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     8881 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     8941 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagcacg
     9001 tactacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     9061 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     9121 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9181 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9241 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     9301 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     9361 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     9421 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     9481 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     9541 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9601 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9661 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9721 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9781 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9841 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     9901 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9961 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10021 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10081 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    10141 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10201 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10261 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10321 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    10381 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    10441 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    10501 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    10561 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    10621 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10681 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10741 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10801 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10861 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10921 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10981 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11041 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11101 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    11161 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    11221 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    11281 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    11341 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    11401 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
