LOCUS       sequence-322599-        7623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of full-length wild-type mouse talin-1 in mammalian 
            cells. Contains C-terminal EGFP tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Azizi L, Cowell AR, Mykuliak VV, Goult BT, Turkki P, Hytonen VP
  TITLE     Cancer associated talin point mutations disorganise cell adhesion 
            and migration.
  JOURNAL   Sci Rep. 2021 Jan 11;11(1):347. doi: 10.1038/s41598-020-77911-4.
  PUBMED    33431906
REFERENCE   2  (bases 1 to 7623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-01-11"; volume: "11(1):347. doi"; pages: " 
            10.1038/s41598-020-77911-4"
COMMENT     Sequence Label: pEGFP_WT-talin1 (1-2541)
FEATURES             Location/Qualifiers
     source          1..7623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggttgcgc tttcgctgaa gattagcatt gggaatgtgg tgaagacgat gcaatttgag
       61 ccatctacca tggtgtacga cgcctgccgc atgattcgtg agcggatccc agaggccctg
      121 gctggccctc ccaacgactt tgggctcttt ctgtcagatg atgaccccaa aaaaggcatc
      181 tggctagagg ctgggaaagc tttggactac tacatgctcc gaaatgggga caccatggag
      241 tacagaaaaa agcagagacc cctgaagatc cggatgttag acggaacggt gaagactatc
      301 atggtggatg actccaagac cgtcacagac atgctcatga ccatttgtgc ccgaattggt
      361 atcaccaacc atgatgagta ttcactggtt cgagagctga tggaagaaaa gaaagatgag
      421 gggacaggga ccctgagaaa agacaagacc ctactgcgag atgaaaagaa gatggagaag
      481 ctaaagcaga agttgcacac agacgatgag ttgaactggc tggaccatgg gcggacactg
      541 agggaacagg gagtggaaga gcatgagacg ttgctgctgc ggagaaagtt cttctactca
      601 gaccagaatg tggattcccg agaccctgta cagctgaacc ttctctatgt gcaggcacga
      661 gatgacatcc tgaatggctc ccatcctgtc tcctttgaca aggcctgtga atttgcaggc
      721 ttccagtgcc agatccagtt tggacctcac aatgaacaga agcacaaggc tggcttcctt
      781 gacctgaagg acttcctgcc caaggagtat gtgaagcaga agggagagcg taagatcttt
      841 caggcacaca agaattgtgg gcagatgagt gaaattgagg ccaaggtacg ctacgtgaag
      901 ctggcccgtt ccctcaagac ttacggtgtc tccttcttcc tagtcaagga aaagatgaag
      961 gggaagaata aactggtgcc caggctgttg ggcatcacta aggagtgtgt gatgcgtgtg
     1021 gatgagaaga ccaaggaggt gattcaggaa tggagcctca ccaacatcaa acgctgggct
     1081 gcctctccca agagctttac tctggacttt ggagactatc aggatggcta ctactcagta
     1141 cagacgacag aaggcgagca gatcgcacag ctcattgctg gctacataga tatcatcctt
     1201 aagaagaaaa aaagcaagga ccattttggg ctggagggag atgaagagtc tactatgttg
     1261 gaggactcag tgtctcccaa aaagtcaaca gtccttcagc agcagtacaa ccgagtgggg
     1321 aaagtggagc acggctctgt ggctctgcca gccatcatgc gctctggagc ctctggtcct
     1381 gagaatttcc aggtggggag catgccacct gcccagcagc agatcaccag tggccagatg
     1441 caccgaggac acatgccacc tctgacttca gcccagcagg cgctcactgg aaccattaac
     1501 tccagcatgc aggctgtgca ggctgcccag gccactctgg atgactttga gactctaccc
     1561 cctcttggcc aggatgctgc ctccaaagct tggcgtaaga acaagatgga tgaatcaaag
     1621 catgagatcc actcccaggt agatgccatc acagctggca ctgcctctgt ggtaaacctg
     1681 acagcaggag atcctgcaga gacagactat acagcagtgg gctgtgcagt caccaccatc
     1741 tcttccaacc tgacggagat gtcccgtggg gtgaagctat tggctgccct gctggaggat
     1801 gaaggcggca atggccggcc ccttctgcaa gcagcaaagg gccttgcagg ggctgtgtcg
     1861 gaactgcttc gcagtgctca gcccgccagc gctgagcctc gtcagaacct gctgcaggca
     1921 gccgggaacg tgggccaggc cagtggggag ctgttgcagc aaattgggga aagtgacact
     1981 gacccccact tccaggatgt tctaatgcag ctagcaaacg cagtggcaag tgctgcagcc
     2041 gccctggtcc tcaaggccaa gagtgtggcc cagcgcacag aggattccgg gcttcagacc
     2101 caagtgattg ctgcagctac gcaatgtgct ctgtccactt cccagctggt ggcctgtacc
     2161 aaggtggtag cacctacaat cagctcacct gtgtgccaag aacagctagt cgaggctggg
     2221 cgactggtgg ccaaagctgt ggagggctgt gtgtctgcct cgcaagcagc tacagaagat
     2281 ggacagctgc ttcgaggggt aggagcagca gccacggctg tcacccaggc cctcaatgag
     2341 ctgctgcagc acgtgaaggc ccacgccacc ggagctgggc ctgccggtcg gtatgaccaa
     2401 gccactgata ccatcctcac cgtcactgaa aacatcttca gctccatggg tgatgctggg
     2461 gagatggtgc gccaggcccg catcctggcc caagccacct cagacctggt caatgctatc
     2521 aaggccgatg ctgaggggga gagtgatctg gagaactcta gaaagctcct gagtgctgcc
     2581 aagatcctcg ctgatgccac cgccaagatg gtggaggcgg ccaagggagc agccgcccac
     2641 cctgacagtg aggaacagca gcagcgactg cgtgaagcag ctgaggggct tcgcatggcc
     2701 accaatgcag ctgcgcagaa cgccatcaag aagaagttgg tgcagcgcct ggagcatgca
     2761 gccaagcaag ctgcagcctc tgcaacacag accattgctg cagcccaaca tgcagcctct
     2821 gcccccaagg cctctgcggg cccccagccc ctactggtgc agagctgtaa ggccgtggca
     2881 gagcagattc cgttgctggt gcagggtgtc cgaggaagtc aagctcagcc tgacagccct
     2941 agtgctcagc tcgccctcat tgctgccagc cagagcttcc tgcagccagg tgggaagatg
     3001 gtggcagcgg caaaagcctc agtgccaaca attcaggacc aggcttcagc gatgcagctg
     3061 agtcagtgcg caaagaacct aggcactgcc ctggccgagc tgcgcactgc tgctcagaag
     3121 gctcaggagg catgtggacc tttggagatg gattctgcac tgagtgttgt acaaaatcta
     3181 gagaaagacc tacaggaaat aaaagcagca gctcgagatg gcaagcttaa gcccttacct
     3241 ggggaaacaa tggagaagtg tacccaagat cttggcaaca gcaccaaagc ggtgagctct
     3301 gccatcgcca agctgctggg ggagattgcc cagggcaacg agaactatgc aggtattgca
     3361 gctcgggatg tagctggtgg actaaggtca ctagcccagg ctgcgcgggg tgtggctgcg
     3421 ctgacatcag atcctgcagt gcaggccatt gtgcttgaca cagccagcga cgttctggac
     3481 aaggccagca gcctcattga agaggcaaaa aaagcatctg gacacccagg ggacccagaa
     3541 agccagcaaa ggcttgctca ggtagctaaa gcagtgaccc aagccctgaa ccgctgtgtc
     3601 agctgcctgc ctggccaaag agacgtggat aatgccctac gagccgttgg agatgccagc
     3661 aagcgactcc tgagtgactt gcttcctcca agcacgggga catttcaaga agcacagagc
     3721 cgattgaatg aagctgcagc tgggttaaat caggccgcca cagaactggt gcaagcctcc
     3781 cgaggaactc ctcaggacct ggctcgggcc tccggccgat ttggacagga cttcagcacc
     3841 ttcctggaag ctggtgtgga gatggccgga caggccccga gccaggagga ccgagcccag
     3901 gtggtgtcca acctgaaggg catatccatg tcttcaagca aacttctcct tgccgctaag
     3961 gccttgtcca cagaccctgc ttctcccaac ctcaagagtc agctggctgc ggctgcccgg
     4021 gcagtgacgg acagcatcaa ccagctcatc accatgtgca cccagcaggc acctggccag
     4081 aaggagtgtg acaatgcact tcggcagcta gagacagtcc gagaactcct ggagaatcca
     4141 gtccagccca tcaacgacat gtcctacttc ggttgcttgg acagtgtcat ggagaactcc
     4201 aaggtcctag gtgaggccat gactggcatc tcccaaaatg ccaagaatgg aaatctgccg
     4261 gagtttggag atgccattgc cacagcctcc aaggctctct gtggcttcac ggaggcagcc
     4321 gcacaggcag catatctagt tggtgtctct gaccccaaca gccaagctgg acagcaagga
     4381 ctggtggaac ccacacagtt tgcccgtgca aaccaggcaa ttcagatggc ctgtcagagt
     4441 ctgggggagc ctggctgtac ccaggcccag gtgttatctg cagccactat tgtagccaaa
     4501 cacacatctg cattgtgtaa cagctgtcgc ctggcttccg ctaggactgc caatcccact
     4561 gccaagcgcc agtttgtaca gtcagccaag gaggtggcca acagtacagc caatcttgtc
     4621 aagaccatca aggcactaga tggggacttc acagaagaga accgtgccca gtgccgagca
     4681 gccacagccc ctctgctgga agctgtggat aacctgagtg cctttgcctc caaccctgag
     4741 ttctccagcg tccctgccca gatcagccct gagggcagag cagccatgga gcccattgta
     4801 atctctgcta agacaatgtt ggagagtgct ggagggctca tccagacagc tcgggcgtta
     4861 gcagtcaatc cccgagaccc cccacgttgg tctgtgctag ctggccactc tcggactgtc
     4921 tcagattcca tcaagaaact tattacaagc atgagggaca aagccccagg gcagctggag
     4981 tgtgagacag ccattgcggc tctgaacagc tgcttgcggg acctagatca ggcttcgctt
     5041 gccgctgtca gccagcagct tgctccccgt gaaggaatct ctcaagaggc tttgcacacc
     5101 cagatgctca ctgcagtgca agaaatttct catctcattg agccgctggc cagcgctgct
     5161 cgggctgaag cctcccagct gggacacaag gtgtcccaaa tggcccagta ctttgaacca
     5221 ctcaccctgg ctgcagtggg tgctgcgtct aagaccctga gccacccgca gcagatggca
     5281 cttctggacc agactaaaac gttggcagag tctgccttgc agttgctata tactgccaag
     5341 gaggctggtg gtaaccccaa gcaagcagca cacacccagg aagccctgga ggaggctgtg
     5401 cagatgatga cagaggccgt agaagacctg acgacgaccc tcaacgaagc agccagtgct
     5461 gcaggagtcg ttggcggcat ggtggactct atcactcagg ccatcaacca gctagatgaa
     5521 ggacctatgg gtgacccaga aggctcattc gtagattacc agacaaccat ggtgaggaca
     5581 gccaaggcca ttgctgtcac tgttcaggag atggtaacca agtcaaacac cagccctgaa
     5641 gagctaggcc ctcttgccaa ccagctgacc agtgactatg gccgactggc ctcacaagcc
     5701 aagcctgcag ctgtggctgc tgaaaatgaa gagataggcg ctcacatcaa acaccgagta
     5761 caggagctgg gccatggctg ctctgctctg gtcaccaagg caggtgcctt gcagtgtagc
     5821 cccagtgatg tctacaccaa gaaggagctc atagagtgtg cccgcagagt gtcagagaag
     5881 gtctcccatg ttctggctgc actccaggct gggaatcgtg gtacccaggc ctgcattaca
     5941 gcagccagtg ctgtgtctgg tatcattgct gacctcgaca ccaccatcat gttcgctact
     6001 gctggcacac ttaaccgtga gggtgctgaa acttttgctg accaccggga gggtatctta
     6061 aagacagcaa aggttcttgt ggaggacacc aaggtcctag tgcagaatgc agctgggagc
     6121 caggagaagt tggcacaagc cgcccagtcc tccgtggcca ccattacccg cctcgctgat
     6181 gtggtcaagc tcggtgcagc cagcctagga gccgaagacc ctgaaactca ggtggtgctg
     6241 atcaatgcag taaaggacgt agccaaggcc ctgggtgacc tcatcagcgc tacgaaggct
     6301 gcagcgggca aagttgggga tgaccctgca gtgtggcagc tcaagaactc tgccaaggtg
     6361 atggtgacca atgtgacatc attgctcaag acagtgaagg ctgtggaaga tgaggccacc
     6421 aaaggcacac gggccctaga ggcaaccaca gagcacatac gtcaggaact ggcggtcttc
     6481 tgttccccag agccacctgc caagacctct acccctgaag acttcatccg aatgaccaag
     6541 ggtattacta tggcaacagc caaagccgtt gctgctggca attcctgtcg acaggaagat
     6601 gtcattgcca cagccaatct gagccgacgg gctattgcgg acatgcttcg ggcttgcaag
     6661 gaagcagctt tccacccaga agtggcgcct gatgtacggc tccgagccct gcactatggc
     6721 cgggagtgtg ccaatggtta cctggaactg ctggaccacg tgctgctgac ccttcagaag
     6781 ccaaacccag acctgaagca gcagctaact ggacactcaa agcgggttgc tggctctgtg
     6841 actgagctca tccaggctgc tgaagccatg aaaggaacag agtgggtgga cccagaggac
     6901 cctactgtca ttgctgagaa tgagctcttg ggagccgcag ccgccatcga ggctgcagcc
     6961 aagaagctgg agcagctgaa gcccagggcc aaacccaagg aggcggatga gtccttgaac
     7021 tttgaggaac aaatcctaga agctgccaag tccatcgctg cagccaccag tgcactggta
     7081 aaggctgcgt cagcagccca gagggagctg gtggctcaag gaaaggtggg cgccattcca
     7141 gccaatgcac tggatgatgg gcagtggtcg cagggcctca tttccgctgc ccgtatggtg
     7201 gctgcggcca ccaacaatct gtgtgaagca gctaacgcag ctgtccaggg ccatgctagc
     7261 caggagaaac tcatttcctc agccaagcaa gtagctgcct ccacagctca gctcctggta
     7321 gcttgcaagg tcaaagcaga tcaggactct gaggcaatga aacggctcca ggctgctggc
     7381 aatgcagtga agagggcctc agataacctg gtaaaggcgg cccagaaagc tgcagccttc
     7441 gaagaccagg agaatgagac ggtggtggtg aaggagaaaa tggttggggg cattgcccag
     7501 attatcgcag cacaggaaga gatgcttcgg aaggaacgag agctggaaga ggctcggaaa
     7561 aagctcgccc agatccggca gcagcagtac aagttcttgc cttcagagct tcgagacgag
     7621 cac
//
