LOCUS       sequence-322622-        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human talin-1 head (residues 1-405) in E. coli. 
            Contains 30 amino acid deletion in F1-loop. N-terminal His6-tag, 
            Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag 
            removal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Kukkurainen S, Azizi L, Zhang P, Jacquier MC, Baikoghli M, von Essen
            M, Tuukkanen A, Laitaoja M, Liu X, Rahikainen R, Orlowski A, Janis 
            J, Maatta JAE, Varjosalo M, Vattulainen I, Rog T, Svergun D, Cheng 
            RH, Wu J, Hytonen VP, Wehrle-Haller B
  TITLE     The F1 loop of the talin head domain acts as a gatekeeper in 
            integrin activation and clustering.
  JOURNAL   J Cell Sci. 2020 Oct 12;133(19). pii: 133/19/jcs239202. doi: 
            10.1242/jcs.239202.
  PUBMED    33046605
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-10-12"; volume: "133(19). pii: 133/19/jcs239202. 
            doi"; pages: " 10.1242/jcs.239202"
COMMENT     Sequence Label: t1-405(del30)
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggttgcac tttcactgaa gatcagcatt gggaatgtgg tgaagacgat gcagtttgag
       61 ccgtctacca tggtgtacga cgcctgccgc atcattcgtg agcggatccc agaggcccca
      121 gctggtcctc ccagcgactt tgggctcttt ctgtcagatg atgaccccaa aaagggtata
      181 tggctggagg ctgggaaagc tttggactac tacatgctcc gaaatgggga cactatggag
      241 tacaggaaga aacagagacc cctgaagatc cgtatgctgg atggaactgt gaagacgatc
      301 atggtggatg actctaagac tgtcactgac atgctcatga ccatctgtgc ccgcattggc
      361 atcaccaatc atgatgaata ttcattggtt cgagagctga tggaagagaa aaaggatgag
      421 ttgaactggc tggaccatgg tcggacactg agggagcagg gtgtagagga gcacgagacg
      481 ctgctgctgc ggaggaagtt cttttactca gaccagaatg tggattcccg ggaccctgta
      541 cagctgaacc tcctgtatgt gcaggcacga gatgacatcc tgaatggctc ccatcctgtc
      601 tcctttgaca aggcctgtga gtttgctggc ttccaatgcc agatccagtt tgggccccac
      661 aatgagcaga agcacaaggc tggcttcctt gacctgaagg acttcctgcc caaggagtat
      721 gtgaagcaga agggagagcg taagatcttc caggcacaca agaattgtgg gcagatgagt
      781 gagattgagg ccaaggtccg ctacgtgaag ctagcccgtt ctctcaagac ttacggtgtc
      841 tccttcttcc tggtgaagga aaaaatgaaa gggaagaaca agctagtgcc caggcttctg
      901 ggcatcacca aggagtgtgt gatgcgagtg gatgagaaga ccaaggaagt gatccaggag
      961 tggaacctca ccaacatcaa acgctgggct gcgtctccca aaagcttcac cctggatttt
     1021 ggagattacc aagatggcta ttactcagta cagacaactg aaggggagca gatcgcacag
     1081 ctcattgctg gctacatcga tatcatcctg aagaagaaaa aaagctaa
//
