LOCUS       sequence-32263-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, 
            Baillie D, Vidal M, Dupuy D
  TITLE     An antibiotic selection marker for nematode transgenesis.
  JOURNAL   Nat Methods. 2010 Sep;7(9):721-3. doi: 10.1038/nmeth.1494. Epub 2010
            Aug 22.
  PUBMED    20729841
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1494"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "721-3"
COMMENT     Sequence Label: pDD04neo pmyo2::gfp
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..49)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(58..82)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttctccttt actcattttt tctaccggta ccttacgctt cttctttgga gcagtcaagc
       61 ctgctttttt gtacaaactt gtcatttctg tgtctgacga tcgagggtta aaatgaaaag
      121 tggtggagag aagtcaaccg ctcggtggga tgcgcgagcc tggagataac tttgattttc
      181 ggcgagccct tctttttccc tgggtgggtg gattgggagg cggggatatc caattgtaat
      241 tctaggtagc tgaaaagtgt ctgcacactg ttctctgacc atcgacgcag gaaaagtctc
      301 tttctcaagt gcaaatagca aaaatcttct ttcttccccc ccatcttccg agcaaggcgc
      361 ccccacggat agttgctcgc tggcggtagg gagcgaagag agcagagaac ctcaacggga
      421 tagtggggat acgagaaaag aaacggcatt ttaggttaac taaagatagt gaagaagccg
      481 gctcaatgca gagaggcaga catccgacgg caaacaacga gagaatttta tccagaacaa
      541 tgagtacagt gtgtgcgcgc aaagtgctct tcctgccatt ttgctactct tatccacaca
      601 accactttag cgattaccaa cctatcttga gacttatctt tattttctct tcttttgccg
      661 acgaaacggg agagaggggc aaaaaatgaa gaatcggggg cactgattaa cacacttact
      721 tttttttagg ctgaagcaca cattgctgtt cggggtgtaa agttcagcag aattcaaagc
      781 aaaagaccac ccgcatcggt agtcaatatt gatctggaag attattaaaa attgtcagag
      841 tgagccatat gcatatgaat ttaattcata cataacattt
//
