LOCUS       sequence-32264-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, 
            Baillie D, Vidal M, Dupuy D
  TITLE     An antibiotic selection marker for nematode transgenesis.
  JOURNAL   Nat Methods. 2010 Sep;7(9):721-3. doi: 10.1038/nmeth.1494. Epub 2010
            Aug 22.
  PUBMED    20729841
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1494"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "721-3"
COMMENT     Sequence Label: pdestRG5273Neo
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(37..139)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    296..395
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             404..424
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(467..495)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
       61 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
      121 acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt
      181 atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta
      241 ttcggcgcaa agtgcgtcgg gtgatgctgc caacttagtc gacgctctag agctccaaat
      301 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt
      361 ttttataatg ccaactttgt acaaaaaagc aggcttgact gctccaaaga agaagcgtaa
      421 ggtaccggta gaaaaaatga gtaaaggaga agaacttttc actggagttg tcccaattct
      481 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      541 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      601 tccatgggta agtttaaaca tatatatact aactaaccct gattatttaa attttcagcc
      661 aacacttgtc actactttct gttatggtgt tcaatgcttc tcgagatacc cagatcatat
      721 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      781 atttttcaaa gatgacggga actacaagac acgtaagttt aaacagttcg gtactaacta
      841 accatacata tttaaatttt caggtgctga agtcaagttt gag
//
