LOCUS       sequence-322650-       10516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 Spike Protein for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10516)
  AUTHORS   Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman 
            J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter 
            G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt 
            AG, Iafrate AJ, Balazs AB
  TITLE     COVID-19-neutralizing antibodies predict disease severity and 
            survival.
  JOURNAL   Cell. 2021 Jan 21;184(2):476-488.e11. doi: 
            10.1016/j.cell.2020.12.015. Epub 2020 Dec 15.
  PUBMED    33412089
REFERENCE   2  (bases 1 to 10516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.015"; journalName: "Cell"; date: 
            "2021-01-21- 21"; volume: "184"; issue: "2"; pages: "476-488.e11"
COMMENT     Sequence Label: pTwist-SARS-CoV-2
FEATURES             Location/Qualifiers
     source          1..10516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      257..393
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    545..616
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(621..667)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    685..733
                     /note="synthetic polyadenylation signal"
     enhancer        814..1117
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1119..1321
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1272..1292
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          1455..1930
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     misc_feature    5881..6469
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5934..5954)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6352..6363)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6522..6643
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6533..6552)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6587..6606
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      6758..6804
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      6834..6970
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    7015..7086
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        7131..7327
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7178..7313
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7341..8135
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7395..7414)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8005..8024
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8146..8370
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(8566..9154)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8646..8665)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(9900..9968,9969..10516,1..244)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10118..10137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
       61 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
      121 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
      181 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
      241 gtaaccgatt ctaggtgcat tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt
      301 atactcccac atatgccaga ttcagcaacg gatacggctt ccccaacttg cccacttcca
      361 tacgtgtcct ccttaccaga aatttatcct taagatcccg aatcgtttaa actcgactct
      421 ggctctatcg aatctccgtc gtttcgagct tacgcgaaca gccgtggcgc tcatttgctc
      481 gtcgggcatc gaatctcgtc agctatcgtc agcttacctt tttggcagcg atcgcggctc
      541 ccgacatctt ggaccattag ctccacaggt atcttcttcc ctctagtggt cataacagca
      601 gcttcagcta cctctcaatt caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt
      661 tatgctatca atcgttgcgt tacacacaca aaaaaccaac acacatccat cttcgatgga
      721 tagcgatttt attatctaac tgctgatcga gtgtagccag atctagtaat caattacggg
      781 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      841 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      901 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      961 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1021 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1081 gcagtacatc tacgtattag tcatcgctat taccatgctg atgcggtttt ggcagtacat
     1141 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     1201 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     1261 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     1321 tggtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     1381 aagacaccgg gaccgatcca gcctcccctc gaagcttaca tgtggtaccg agctcggatc
     1441 ctgagaactt cagggtgagt ctatgggacc cttgatgttt tctttcccct tcttttctat
     1501 ggttaagttc atgtcatagg aaggggagaa gtaacagggt acacatattg accaaatcag
     1561 ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt
     1621 atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat
     1681 catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaatagc
     1741 aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata
     1801 ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg
     1861 ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt
     1921 cctcccacag ctcctgggca acgtgctggt ctgtgtgctg gcccatcact ttggcaaagg
     1981 cggccgccat gtttgtgttc ctcgttctcc tgcccctcgt ttcttcccaa tgcgtgaacc
     2041 tcacaactag aacgcagctc ccacccgctt acacgaactc ttttactcgt ggagtatact
     2101 atcccgataa agtctttagg tccagtgtcc ttcattctac tcaagacctt ttcctgccat
     2161 tcttctcaaa tgtcacctgg ttccatgcta tccacgtttc tggcaccaac ggaactaaac
     2221 gatttgataa tcccgtcttg ccatttaacg atggcgtata ctttgcctca acggagaagt
     2281 ccaacattat ccggggatgg atatttggca ccaccctgga ctccaagaca cagtctctcc
     2341 tcattgtgaa caacgccacc aatgtcgtca tcaaagtctg tgaatttcaa ttttgtaacg
     2401 atccctttct cggggtttat tatcataaga ataacaagtc ttggatggag tcagagttcc
     2461 gcgtttacag ttccgctaac aactgtactt ttgagtacgt ttcccaacca tttctcatgg
     2521 atcttgaagg gaagcaaggc aacttcaaga atctccgcga atttgtgttt aagaatatag
     2581 acggctactt caaaatttat agcaagcata ccccgataaa tctggttcgg gatctccctc
     2641 aaggcttctc cgcgttggaa cccctcgtgg acttgccgat tggcatcaac atcacccggt
     2701 tccaaaccct tcttgccctt catcgaagct acctcacgcc tggcgacagt agtagtggtt
     2761 ggacagcggg cgcagcagcc tactatgtgg gatatttgca accacgcacc tttcttctca
     2821 aatataacga aaacggaaca attaccgatg ccgtagactg tgccctcgat cccctgtccg
     2881 aaactaaatg cacactcaag tccttcacag ttgagaaagg catataccaa accagtaact
     2941 tccgcgtcca acccaccgaa tccattgttc gattccctaa catcaccaat ttgtgtcctt
     3001 tcggtgaagt atttaatgcc actcgatttg catcagttta tgcctggaat cgcaagcgaa
     3061 tatcaaattg tgttgcagat tatagtgtcc tgtataatag cgcatcattc agcactttta
     3121 agtgttatgg ggtcagccct acgaaattga atgacttgtg ttttaccaac gtttacgcag
     3181 attctttcgt catacgcggc gacgaagtcc gacagattgc accggggcaa accggaaaga
     3241 tagcagacta taattataag ttgccggatg acttcaccgg ctgcgttata gcatggaact
     3301 ccaataatct cgattcaaaa gtcggcggca actataatta tctttaccgc ctgtttcgca
     3361 agtcaaatct gaagcccttt gaacgtgaca tctccaccga aatttatcaa gctggcagca
     3421 ccccatgtaa tggagtggaa gggtttaact gttattttcc ccttcaatca tacggatttc
     3481 aaccaacaaa tggggtgggc tatcaaccat atcgggtggt agtcttgagc tttgagctgc
     3541 tccacgcacc ggctacagtt tgcggaccga agaaaagcac taacctggtg aagaataaat
     3601 gtgtgaactt caactttaat gggctcaccg gaacaggggt gctgacagaa tcaaacaaga
     3661 aatttctccc ttttcaacaa tttggtagag acattgccga taccacagat gccgtccgcg
     3721 atccgcaaac actggaaatc ctcgatataa caccctgtag cttcggcggg gtcagcgtga
     3781 taactcccgg aacgaacaca tccaaccaag tggctgtcct gtatcaagat gtcaactgta
     3841 ccgaagtccc tgtggcgata cacgccgacc aactgacacc cacctggaga gtctactcaa
     3901 caggttccaa tgtctttcaa actagagcgg gttgtctcat tggcgctgag catgtcaata
     3961 attcatacga atgtgatatt cctattgggg caggaatctg tgcgtcttat caaactcaaa
     4021 cgaactcacc acggcgcgct cgctcagtag ccagccagtc cataatcgcc tataccatga
     4081 gtctcggggc tgagaactcc gtggcttatt caaacaattc catcgcaatc ccaactaact
     4141 ttaccattag cgttaccacg gaaatcttgc ccgtcagcat gactaagacg agcgtagact
     4201 gtacgatgta tatctgtggg gattccaccg agtgtagcaa cctcttgctt caatacgggt
     4261 ctttctgtac tcaactcaac cgagcattga ccggaattgc ggtcgaacaa gacaagaata
     4321 cccaagaggt gttcgcacaa gtaaagcaaa tatacaaaac gccgccaatc aaagattttg
     4381 gcgggtttaa ttttagccaa atactgccag acccatctaa gccttcaaaa cgcagcttta
     4441 tcgaggatct gctgttcaac aaagttacgt tggctgacgc gggtttcatt aagcagtatg
     4501 gggattgttt gggagacatt gcggcccgcg accttatctg tgctcaaaaa ttcaatgggt
     4561 tgacagttct gccccctctg ctcactgatg agatgattgc acagtataca tcagcattgt
     4621 tggcaggaac cattacatcc ggctggactt ttggcgcggg tgcggccctt caaatacctt
     4681 tcgctatgca aatggcttac agattcaatg ggattggtgt cacgcaaaat gtattgtatg
     4741 aaaatcagaa gctgatcgct aatcaattta acagcgcgat tggcaagatc caagattcac
     4801 tgtcaagcac agcgtccgcc ctcggcaaac tccaagatgt tgtgaatcaa aacgcccagg
     4861 ccctgaatac cctcgtgaag caactgagtt ctaacttcgg cgcaatctcc agcgtcctca
     4921 acgacatatt gtccagactt gataaggtgg aagccgaggt gcaaattgat cggctcatca
     4981 caggcaggct ccagtcactc caaacttatg tgacacaaca gttgatccgg gctgctgaga
     5041 tacgagcaag cgccaacctg gccgccacta aaatgtcaga gtgcgtgctc gggcagtcca
     5101 aacgggttga cttttgtggc aagggctatc acttgatgtc cttcccccaa tccgccccac
     5161 atggagtggt ctttctccac gtcacttatg tccctgctca agagaagaac tttacgacag
     5221 ctccagcaat ctgtcacgat ggcaaagcgc actttcccag ggaaggcgtt ttcgtcagta
     5281 atggcacgca ttggtttgtg acccaaagga atttctatga accccaaatc ataaccactg
     5341 acaatacatt tgtctcaggc aactgtgatg tggtcattgg gatcgtgaac aacacggtat
     5401 atgatcccct gcaacccgag ttggatagct tcaaagagga attggataaa tactttaaga
     5461 atcatacttc acccgatgtt gatctcgggg acatatcagg aataaatgca agtgtcgtca
     5521 acattcagaa agagattgat cgccttaatg aggtcgccaa gaacttgaat gaatctttga
     5581 tcgacttgca agaactgggc aaatacgaac aatatattaa atggccctgg tacatttggt
     5641 tgggctttat cgccggactt atagctatcg ttatggtcac aattatgttg tgctgtatga
     5701 ctagctgttg ttcctgcttg aaaggatgtt gcagttgcgg atcttgttgt aaatttgacg
     5761 aagatgattc tgagcctgta ctcaagggag tcaaactgca ttacacctaa aggatcctct
     5821 agaagttgtc tcctcctgca ctgactgact gatacaatcg atttctggat ccgcaggcct
     5881 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5941 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6001 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6061 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6121 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6181 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6241 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     6301 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6361 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6421 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtctagctt
     6481 gactgactga gatacagcgt accttcagct cacagacatg ataagataca ttgatgagtt
     6541 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     6601 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     6661 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     6721 ctacaaatgt ggtattggcc catctctatc ggtatcgtag cataacccct tggggcctct
     6781 aaacgggtct tgaggggttt tttgtgcccc tcgggccgga ttgctatcta ccggcattgg
     6841 cgcagaaaaa aatgcctgat gcgacgctgc gcgtcttata ctcccacata tgccagattc
     6901 agcaacggat acggcttccc caacttgccc acttccatac gtgtcctcct taccagaaat
     6961 ttatccttaa ggtcgtcagc tatcctgcag gcgatctctc gatttcgatc aagacattcc
     7021 tttaatggtc ttttctggac accactaggg gtcagaagta gttcatcaaa ctttcttccc
     7081 tccctaatct cattggttac cttgggctat cgaaacttaa ttaagcgatc tgcatctcaa
     7141 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7201 ttccgcccat tctccgcccc atcgctgact aatttttttt atttatgcag aggccgaggc
     7261 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7321 ttgcaaagga ggtagccaac atgattgaac aagatggatt gcacgcaggt tctcccgccg
     7381 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     7441 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     7501 ccggtgccct gaatgaactc caggacgagg cagcgcggct atcgtggctg gccacgacgg
     7561 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     7621 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     7681 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     7741 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     7801 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     7861 tcaaggcgcg gatgcccgac ggcgaggatc tcgtcgtgac ccacggcgat gcctgcttgc
     7921 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     7981 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     8041 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     8101 tcgccttcta tcgccttctt gacgagttct tctagtatgt aagccctgtg ccttctagtt
     8161 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     8221 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     8281 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     8341 ggcatgctgg ggatgcggtg ggctctatgg ttaattaacc agtcaagtca gctacttggc
     8401 gagatcgact tgtctgggtt tcgactacgc tcagaattgc gtcagtcaag ttcgatctgg
     8461 tccttgctat tgcacccgtt ctccgattac gagtttcatt taaatcatgt gagcaaaagg
     8521 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8581 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8641 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8701 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8761 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8821 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8881 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8941 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9001 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9061 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9121 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9181 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9241 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9301 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9361 gatctgtcta tttcgttcat ccatagttgc atttaaattt ccgaactctc caaggccctc
     9421 gtcggaaaat cttcaaacct ttcgtccgat ccatcttgca ggctacctct cgaacgaact
     9481 atcgcaagtc tcttggccgg ccttgcgcct tggctattgc ttggcagcgc ctatcgccag
     9541 gtattactcc aatcccgaat atccgagatc gggatcaccc gagagaagtt caacctacat
     9601 cctcaatccc gatctatccg agatccgagg aatatcgaaa tcggggcgcg cctggtgtac
     9661 cgagaacgat cctctcagtg cgagtctcga cgatccatat cgttgcttgg cagtcagcca
     9721 gtcggaatcc agcttgggac ccaggaagtc caatcgtcag atattgtact caagcctggt
     9781 cacggcagcg taccgatctg tttaaaccta gatattgata gtctgatcgg tcaacgtata
     9841 atcgagtcct agcttttgca aacatctatc aagagacagg atcagcagga ggctttcgca
     9901 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9961 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcgc
    10021 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    10081 aagaacgctt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10141 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10201 ttgagtattc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10261 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgattg
    10321 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10381 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10441 ctgtagcaat ggcaacaacc ttgcgtaaac tattaactgg cgaactactt actctagctt
    10501 cccggcaaca gttgat
//
