LOCUS       sequence-322676-        9597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP tagged Man2a1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Sun X, Mahajan D, Chen B, Song Z, Lu L
  TITLE     A quantitative study of the Golgi retention of glycosyltransferases.
  JOURNAL   J Cell Sci. 2021 Oct 15;134(20). pii: 272560. doi: 
            10.1242/jcs.258564. Epub 2021 Oct 21.
  PUBMED    34533190
REFERENCE   2  (bases 1 to 9597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-15"; volume: "134(20). pii: 272560. doi"; 
            pages: " 10.1242/jcs.258564. Epub 2021 Oct 21"
COMMENT     Sequence Label: Man2a1-GFP
FEATURES             Location/Qualifiers
     source          1..9597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        71..450
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        451..662
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     613..633
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     659..683
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          823..955
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     938..957
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1000..1018
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4471..4473,4474..5187)
                     /codon_start=1
                     /product="monomeric GFP variant optimized for use in 
                     oxidizing environments such as the secretory pathway 
                     (Costantini et al., 2015)"
                     /label=moxGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQSFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4513..4534)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    5234..5355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5245..5264)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5299..5318
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5541..5996
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5628..5647)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5838..5859
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6113..6470
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6113..6304
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6136..6156)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6321..6456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6521..7315
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6575..6594)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7185..7204
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7379..7427
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7468..7487)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7587..7609
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7647..7665)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7733..7837
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7838..7906,7907..8698)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8056..8075)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8869..9457
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9358..9377
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga
       61 ccgccatgtt ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      121 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      181 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      241 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      301 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
      361 tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac
      421 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
      481 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      541 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctgcgatcgc
      601 ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      661 ctcgtttagt gaaccgtcag atcactagaa gctttattgc ggtagtttat cacagttaaa
      721 ttgctaacgc agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agtgactctc
      781 ttaaggtagc cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca
      841 agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt
      901 ttctgatagg cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc
      961 cactcccagt tcaattacag ctcttaaggc tagagtactt aatacgactc actataggct
     1021 agcctcgagg ccaccatgaa gttaagccgc cagttcaccg tgttcggcag tgcgatcttc
     1081 tgtgtggtga ttttctcgct ctacctgatg ctggaccggg gtcacttaga ctaccccagg
     1141 aacccgcgcc gcgagggctc cttccctcag ggccagctct caatgttgca agaaaaaata
     1201 gaccatttgg agcgtttgct agctgagaat aatgagatca tctcaaatat tagagactca
     1261 gtcatcaatt tgagtgagtc tgtggaggat ggtccgaaaa gttcacaaag caatttcagc
     1321 caaggtgctg gctcacatct tctgccctca caattatccc tctcagttga cactgcagac
     1381 tgtctgtttg cttcacaaag tggaagtcac aattcagatg tgcagatgtt ggatgtttac
     1441 agtctaattt cttttgacaa tccagatggt ggagtttgga agcaaggatt tgacattact
     1501 tatgaatcta atgaatggga cactgaaccc cttcaagtct ttgtggtgcc tcattcccat
     1561 aacgacccag gttggttgaa gactttcaat gactacttta gagacaagac tcagtatatt
     1621 tttaataaca tggtcctaaa gctgaaagaa gactcacgga ggaagtttat ttggtctgag
     1681 atctcttacc tttcaaagtg gtgggatatt atagatattc agaagaagga tgctgttaaa
     1741 agtttaatag aaaatggtca gcttgaaatt gtgacaggtg gctgggttat gcctgatgaa
     1801 gctactccac attattttgc cttaattgat caactaattg aaggacatca gtggctggaa
     1861 aataatatag gagtgaaacc tcggtccggc tgggctattg atccctttgg acactcacca
     1921 acaatggctt atcttctaaa ccgtgctgga ctttctcaca tgcttatcca gagagttcat
     1981 tatgcagtta aaaaacactt tgcactgcat aaaacattgg agtttttttg gagacagaat
     2041 tgggatctgg gatctgtcac agatatttta tgccacatga tgcccttcta cagctatgac
     2101 atccctcaca cttgtggacc tgatcctaaa atatgctgcc agtttgattt taaacgtctt
     2161 cctggaggca gatttggttg tccctgggga gtccccccag aaacaataca tcctggaaat
     2221 gtccaaagca gggctcggat gctactagat cagtaccgaa agaagtcaaa gctttttcgt
     2281 accaaagttc tcctggctcc actaggagat gatttccgct actgtgaata cacggaatgg
     2341 gatttacagt ttaagaatta tcagcagctt tttgattata tgaattctca gtccaagttt
     2401 aaagttaaga tacagtttgg aactttatca gatttttttg atgcgctgga taaagcagat
     2461 gaaactcaga gagacaaggg ccagtcgatg ttccctgttt taagtggaga ttttttcact
     2521 tatgccgatc gagatgatca ttactggagt ggctatttta catccagacc cttttacaaa
     2581 cgaatggaca gaatcatgga atctcattta agggctgctg aaattcttta ctatttcgcc
     2641 ctgagacaag ctcacaaata caagataaat aaatttctct catcatcact ttacacggca
     2701 ctgacagaag ccagaaggaa tttgggactg tttcaacatc atgatgctat cacaggaact
     2761 gcaaaagact gggtggttgt ggattatggt accagacctt ttcattcgtt aatggttttg
     2821 gagaagataa ttggaaattc tgcatttctt cttattttga aggacaaact cacatacgac
     2881 tcttactctc ctgatacctt cctggagatg gatttgaaac aaaaatcaca agattctctg
     2941 ccacaaaaaa atataataag gctgagtgcg gagccaaggt accttgtggt ctataatcct
     3001 ctagaacaag accgaatctc gttggtctca gtctatgtga gttccccgac agtgcaagtg
     3061 ttctctgctt caggaaaacc tgtggaagtt caagtcagcg cagtttggga tacagcaaat
     3121 actatttcag aaacagccta tgagatctct tttcgagcac atataccgcc attgggactg
     3181 aaagtgtata agattttgga atcagcaagt tcaaattcac atttagctga ttatgtcttg
     3241 tataagaata aagtagaaga tagcggaatt ttcaccataa agaatatgat aaatactgaa
     3301 gaaggtataa cactagagaa ctcctttgtt ttacttcggt ttgatcaaac tggacttatg
     3361 aagcaaatga tgactaaaga agatggtaaa caccatgaag taaatgtgca attttcatgg
     3421 tatggaacca caattaaaag agacaaaagt ggtgcctacc tcttcttacc tgatggtaat
     3481 gccaagcctt atgtttacac aacaccgccc tttgtcagag tgacacatgg aaggatttat
     3541 tcggaagtga cttgcttttt tgaccatgtt actcatagag tccgactata ccacatacag
     3601 ggaatagaag gacagtctgt ggaagtttcc aatattgtgg acatccgaaa agtatataac
     3661 cgtgagattg caatgaaaat ttcttctgat ataaaaagcc aaaatagatt ttatactgac
     3721 ctaaatgggt accagattca acctagaatg acactgagca aattgcctct tcaagcaaat
     3781 gtctatccca tgaccacaat ggcctatatc caggatgcca aacatcgttt gacactgctc
     3841 tctgctcagt ctttaggggt ttcgagtttg aatagtggtc agattgaagt tatcatggat
     3901 cgaagactca tgcaagatga taatcgtggc cttgagcaag gtatccagga taacaagatt
     3961 acagctaatc tatttcgaat actactagaa aaaagaagtg ctgttaatac ggaagaagaa
     4021 aagaagtcgg tcagttatcc ttctctcctt agccacataa cttcttctct catgaatcat
     4081 ccagtcattc caatggcaaa taagttctca cctacccttg agctgcaagg tgaattctct
     4141 ccattacagt catctttgcc ttgtgacatt catctggtta atttgagaac aatacagtca
     4201 aaggtgggca atgggcactc caatgaggca gccttgatcc tccacagaaa agggtttgat
     4261 tgtcggttct ctagcaaagg cacagggctg ttttgttcta ctactcaggg aaagatattg
     4321 gtacagaaac ttttaaacaa gtttattgtc gaaagtctca caccttcatc actatccttg
     4381 atgcattcac ctcccggcac tcagaatata agtgagatca acttgagtcc aatggaaatc
     4441 agcacattcc gaatccagtt gaggccgcgg gtgtccaagg gcgaggagct gttcaccggg
     4501 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt ctccgtgcgg
     4561 ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat cagcaccacc
     4621 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagagc
     4681 ttctcccgct accccgacca catgaagcgc cacgacttct tcaagagcgc catgcccgaa
     4741 ggctacgtcc aggagcgcac catctccttc aaggacgacg gcacctacaa gacccgcgcc
     4801 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     4861 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaactc ccacaacgtc
     4921 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     4981 gtggaggacg gctccgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     5041 ggccccgtgc tgctgcccga caaccactac ctgtccaccc agtccaagct gtccaaagac
     5101 cccaacgaga agcgcgatca catggtcctt ctggaattcg tgaccgccgc cgggatcact
     5161 cacggcatgg acgagctgta caagtaagcg gccgcttccc tttagtgagg gttaatgctt
     5221 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     5281 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     5341 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     5401 atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat ccgataagga
     5461 tcgatccggg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     5521 gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5581 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5641 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     5701 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     5761 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5821 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5881 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5941 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg
     6001 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg cggatctgcg
     6061 cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg
     6121 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttgatt
     6481 cttctgacac aacagtctcg aacttaaggc tagagccacc atgattgaac aagatggatt
     6541 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     6601 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     6661 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     6721 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     6781 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     6841 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     6901 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     6961 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     7021 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     7081 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     7141 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     7201 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     7261 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     7321 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg atggccgcaa
     7381 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat
     7441 aaggatccgc gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     7501 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     7561 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     7621 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     7681 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     7741 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7801 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7861 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7921 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7981 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     8041 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     8101 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     8161 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8221 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8281 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8341 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8401 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8461 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8521 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8581 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8641 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8701 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8761 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8821 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8881 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8941 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9001 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9061 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9121 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9181 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9241 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9301 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9361 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9421 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9481 acggttcctg gccttttgct ggccttttgc tcacatggct cgacagatct tcaatattgg
     9541 ccattagcca tattattcat tggttatata gcataaatca atattggcta ttggcca
//
