LOCUS       sequence-32270-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, 
            Baillie D, Vidal M, Dupuy D
  TITLE     An antibiotic selection marker for nematode transgenesis.
  JOURNAL   Nat Methods. 2010 Sep;7(9):721-3. doi: 10.1038/nmeth.1494. Epub 2010
            Aug 22.
  PUBMED    20729841
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1494"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "721-3"
COMMENT     Sequence Label: pdestDD04Neo
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..65
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     49..65
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(99..115)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(100..116)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gatacctcct aagggacaaa gctggagctc caccgcggtg gcggccgctc tagaactagt
       61 ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagaaa
      121 cagttatgtt tggtatattg ggaatgtatt ctgtcattta aggcaaaaac cccatagaca
      181 ctactccact ttcaaattat caacttctac tcaccttcca ctgagcctca aacccaaacc
      241 ttcttccgat ctttcttgca tcgtgctcat caatacttgt gattttagca agaaaaacgc
      301 cgtcctcgaa atcttgacaa acaatagggg aaaccccaaa aagcaaaagc aggaaaatta
      361 atttcataaa ctttcacttg attgaaaaat tccaaaaata ctccaaaact ttttcacgat
      421 gagcatgatt tgacgtcatg agaggcccag acgtgcgaag aaataaaaat tgcggtcata
      481 aactgaaacg taacatatga taaggtattt tgtgtgcggt tttttctatg atgtttcaaa
      541 aaaatttatc agaagtaaaa aacataagaa gtgtacacag aacattgtgt agattttggg
      601 atagaattat tattaatggg gggagggagc acaatttttt tcgacttttt attacgaatt
      661 aattctattt ttgaatctac acaatttcat tgttagaggt ggcttaaaag aagctaaaaa
      721 acaaagaaat taagagaagt tttttgataa taacaaaaat agggggtggg agcacaggga
      781 gaaagagcat gtagggatgt tgaagagtaa ttggactcag aagaactcgt caagaaggcg
      841 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
      901 agcccattcg ccgcca
//
