LOCUS       sequence-32276-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLKA-J72016
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(192..296)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    343..367
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     429..446
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(473..490)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(614..638)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
       61 taacccactc gtgcacccaa ctgatcctct gcatctttta ctttcaccag cgtttctggg
      121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      301 tttccccgaa aagtgccacc tgacgtctaa gagatccgat caaccacttt gtacaagaaa
      361 gctgggtcga attgattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
      421 ccgagcgcag cgagtcagtg agcgaggaag cctgaggagc gagttaggat ccgtgatggt
      481 gatgatggtg agaatccata tgtaacttct tagatgctag gtgttgtaga gatctcatga
      541 attccacttc agatccttag cgaaagctaa ggattttttt tatctgaaat tctgcctcgt
      601 gataccaatt cggagcctgc ttttttgtac aaacttgttg ataatggcaa ttcttgatcg
      661 ggagatccgt gattcgcacg ggcccatggc taattcccat gtcagccgtt aagtgttcct
      721 gtgtcactca aaattgcttt gagaggctct aagggcttct cagtgcgtta catccctggc
      781 ttgttgtcca caaccgttaa accttaaaag ctttaaaagc cttatatatt c
//
