LOCUS       sequence-322770-        6045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization of mGreenLantern fluorescent protein to tubulin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Campbell BC, Nabel EM, Murdock MH, Lao-Peregrin C, Tsoulfas P, 
            Blackmore MG, Lee FS, Liston C, Morishita H, Petsko GA
  TITLE     mGreenLantern: a bright monomeric fluorescent protein with rapid 
            expression and cell filling properties for neuronal imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Dec 1;117(48):30710-30721. doi: 
            10.1073/pnas.2000942117. Epub 2020 Nov 18.
  PUBMED    33208539
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.2000942117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-12-1- 1"; volume: "117"; issue: "48"; pages: 
            "30710-30721"
COMMENT     Sequence Label: Tubulin-mGreenLantern
FEATURES             Location/Qualifiers
     source          1..6045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      243..252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(249..251,252..254,255..965)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(294..315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    2469..2590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2506..2525)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2560..2579
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2597..3052)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2734..2755)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2946..2965
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3079..3183
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3185..3542
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3185..3376
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3208..3228)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3393..3528
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3577..4371
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3577..4371
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3631..3650)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4241..4260
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4559..4578)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4603..4650
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4979..5567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5468..5487
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5742..6045
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      301 agctggacgg cgacgtaaac ggccacaagt tcagcgtccg cggcgagggc gagggcgatg
      361 ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      421 ggcccaccct cgtgaccacc ttaggctacg gcgtggcctg cttcgcccgc taccccgacc
      481 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      541 ccatctcttt caaggacgac ggtacctaca agacccgcgc cgaggtgaag ttcgagggcg
      601 acaccctggt gaaccgcatc gtgctgaagg gcatcgactt caaggaggac ggcaacatcc
      661 tggggcacaa gctggagtac aacttcaaca gccacaaggt ctatatcacg gccgacaagc
      721 agaagaacgg catcaaggct aacttcaaga cccgccacaa cgttgaggac ggcggcgtgc
      781 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      841 acaaccacta cctgagccat cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
      901 acatggtcct gaaggagagg gtgaccgccg ccgggattac acatgacatg gacgagctgt
      961 acaagtccgg actcagatct cgagtgcgtg agtgcatctc catccacgtt ggccaggctg
     1021 gtgtccagat tggcaatgcc tgctgggagc tctactgcct ggaacacggc atccagcccg
     1081 atggccagat gccaagtgac aagaccattg ggggaggaga tgactccttc aacaccttct
     1141 tcagtgagac gggcgctggc aagcacgtgc cccgggctgt gtttgtagac ttggaaccca
     1201 cagtcattga tgaagttcgc actggcacct accgccagct cttccaccct gagcagctca
     1261 tcacaggcaa ggaagatgct gccaataact atgcccgagg gcactacacc attggcaagg
     1321 agatcattga ccttgtgttg gaccgaattc gcaagctggc tgaccagtgc accggtcttc
     1381 agggcttctt ggttttccac agctttggtg ggggaactgg ttctgggttc acctccctgc
     1441 tcatggaacg tctctcagtt gattatggca agaagtccaa gctggagttc tccatttacc
     1501 cagcacccca ggtttccaca gctgtagttg agccctacaa ctccatcctc accacccaca
     1561 ccaccctgga gcactctgat tgtgccttca tggtagacaa tgaggccatc tatgacatct
     1621 gtcgtagaaa cctcgatatc gagcgcccaa cctacactaa ccttaaccgc cttattagcc
     1681 agattgtgtc ctccatcact gcttccctga gatttgatgg agccctgaat gttgacctga
     1741 cagaattcca gaccaacctg gtgccctacc cccgcatcca cttccctctg gccacatatg
     1801 cccctgtcat ctctgctgag aaagcctacc atgaacagct ttctgtagca gagatcacca
     1861 atgcttgctt tgagccagcc aaccagatgg tgaaatgtga ccctcgccat ggtaaataca
     1921 tggcttgctg cctgttgtac cgtggtgacg tggttcccaa agatgtcaat gctgccattg
     1981 ccaccatcaa aaccaagcgc agcatccagt ttgtggattg gtgccccact ggcttcaagg
     2041 ttggcatcaa ctaccagcct cccactgtgg tgcctggtgg agacctggcc aaggtacaga
     2101 gagctgtgtg catgctgagc aacaccacag ccattgctga ggcctgggct cgcctggacc
     2161 acaagtttga cctgatgtat gccaagcgtg cctttgttca ctggtacgtg ggtgagggga
     2221 tggaggaagg cgagttttca gaggcccgtg aagatatggc tgcccttgag aaggattatg
     2281 aggaggttgg tgtggattct gttgaaggag agggtgagga agaaggagag gaatactaag
     2341 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
     2401 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     2461 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     2521 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     2581 atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     2641 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     2701 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     2761 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     2821 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     2881 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     2941 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     3001 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     3061 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3121 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3181 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     3241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     3301 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3361 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3421 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3481 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     3541 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     3601 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     3661 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     3721 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     3781 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     3841 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     3901 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     3961 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     4021 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     4081 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     4141 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     4201 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     4261 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     4321 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     4381 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     4441 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     4501 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     4561 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     4621 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     4681 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     4741 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     4801 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt
     4861 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4921 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4981 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5041 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5101 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5161 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5221 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5281 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5341 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5401 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5461 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5521 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5581 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5641 tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca
     5701 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     5761 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     5821 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     5881 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     5941 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     6001 cctacttggc agtacatcta cgtattagtc atcgctatta ccatg
//
