LOCUS       sequence-322776-       14555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Delivery vector to insert the miniTn6900(KanR) element into the E. 
            coli chromosome at the neutral Tn7 attachment site inside a miniTn7 
            element..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14555)
  AUTHORS   Petassi MT, Hsieh SC, Peters JE
  TITLE     Guide RNA Categorization Enables Target Site Choice in 
            Tn7-CRISPR-Cas Transposons.
  JOURNAL   Cell. 2020 Nov 30. pii: S0092-8674(20)31464-1. doi: 
            10.1016/j.cell.2020.11.005.
  PUBMED    33271061
REFERENCE   2  (bases 1 to 14555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Nov 30. pii: S0092-8674(20)31464-1. doi: 
            10.1016/j.cell.2020.11.005."
COMMENT     Sequence Label: pMTP112 (pMS26_miniTn6900(KanR))
FEATURES             Location/Qualifiers
     source          1..14555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3448..3465)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3618..3704
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3796..3823
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3842..3933
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3934..4002,4003..4794)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4152..4171)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(4923..4942)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     oriT            4965..5074
                     /note="incP origin of transfer"
     primer_bind     complement(5112..5131)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      5814..6036
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             6084..7034
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     mobile_element  7467..7632
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     terminator      7743..7770
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(8082..8101)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             8093..8908
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             8093..8908
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(8166..8185)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(9323..9441)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     terminator      9526..9612
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9704..9731
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(10123..11001)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        11028..11312
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     11235..11254
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ccattaggta cgcatttgct actaagtgag cgtatttcgg tcatgattcg aggtggctac
       61 gtaggcagaa atcctaaaac aggagattta caaaagcatt tacaaaatgg ttatgagcgt
      121 gttcaaacgg gagagttgga gacatttcgc tttgaggagg cacgatctac ggcacaaagc
      181 ttattgttaa ttggttgttc tggtagtggg aagacgacct ctcttcatcg tattctagcc
      241 acgtatcctc aggtgattta ccatcgtgaa ctcaatgtag agcaggtggt gtatttgaaa
      301 atagactgct cgcataatgg ttcgctaaaa gaaatctgct tgaatttttt cagagcgttg
      361 gatcgagcct tgggctcgaa ctatgagcgt cgttatggct taaaacgtca tggtatagaa
      421 accatgttgg ctttgatgtc gcaaatagcc aatgcacatg ctttagggtt gttggttatt
      481 gatgaaattc agcatttaag ccgctctcgt tcgggtggat ctcaagagat gctgaacttt
      541 tttgtgacga tggtgaatat tattggcgta ccagtgatgt tgattggtac ccctaaagca
      601 cgagagattt ttgaggctga tttgcggtct gcacgtagag gggcagggtt tggagctata
      661 ttctgggatc ctatacaaca aacgcaacgt ggaaagccca atcaagagtg gatcgctttt
      721 acggataatc tttggcaatt acagctttta caacgcaaag atgcgctgtt atcggatgag
      781 gtccgtgatg tgtggtatga gctaagccaa ggagtgatgg acattgtagt aaaacttttt
      841 gtactcgctc agctccgtgc gctagcttta ggcaatgagc gtattaccgc tggtttattg
      901 cggcaagtgt atcaagatga gttaaagcct gtgcacccca tgctagaggc attacgctcg
      961 ggtatcccag aacgcattgc tcgttattct gatctagtcg ttcccgagat tgataaacgg
     1021 ttaatccaac ttcagctaga tatcgcagcg atacaagaac aaacaccaga agaaaaagcc
     1081 cttcaagagt tagataccga agatcagcgt catttatatc tgatgctgaa agaggattac
     1141 gattcaagcc tgttaattcc cactattaaa aaagcgttta gccagaatcc aacgatgaca
     1201 agacaaaagt tactgcctct tgttttgcag tggttgatgg aaggcgaaac ggtagtgtca
     1261 gaactagaaa agccctccaa gagtaaaaag gtttcggcta taaaggtagt caagcccagc
     1321 gactgggata gcttgcctga tacggattta cgttatatct attcacaacg ccaacctgaa
     1381 aaaaccatgc atgaacggtt aaaagggaaa ggggtaatag tggatatggc gagcttattt
     1441 aaacaagcag gttagccatg agaaactttc ctgttccgta ctcgaatgag ctgatttata
     1501 gcactattgc acgggcaggc gtttatcaag ggattgttag tcctaagcag ctgttggatg
     1561 aggtgtatgg caaccgcaag gtggtcgcta ccttaggtct gccctcgcat ttaggtgtga
     1621 tagcaagaca tctacatcaa acaggacgtt acgctgttca gcagcttatt tatgagcata
     1681 ccttattccc tttatatgct ccgtttgtag gcaaggagcg ccgagacgaa gctattcggt
     1741 taatggagta ccaagcgcaa ggtgcggtgc atttaatgct aggagtcgct gcttctagag
     1801 ttaagagcga taaccgcttt agatactgcc ctgattgcgt tgctcttcag ctaaataggt
     1861 atggggaagc cttttggcaa cgagattggt atttgcccgc tttgccatat tgtccaaaac
     1921 acggtgcttt agtcttcttt gatagagctg tagatgatca ccgacatcaa ttttgggctt
     1981 tgggtcatac tgagctgctt tcagactacc ccaaagactc cctatctcaa ttaacagcac
     2041 tagctgctta tatagcccct ctgttagatg ctccacgagc gcaagagctt tccccaagcc
     2101 ttgagcagtg gacgctgttt tatcagcgct tagcgcagga tctagggcta accaaaagca
     2161 agcacattcg tcatgacttg gtggcggaga gagtgaggca gacttttagt gatgaggcac
     2221 tagagaaact ggatttaaag ttggcagaga acaaggacac gtgttggctg aaaagtatat
     2281 tccgtaagca tagaaaagcc tttagttatt tacagcatag tattgtgtgg caagccttat
     2341 tgccaaaact aacggttata gaagcgctac agcaggcaag tgctcttact gagcactcta
     2401 taacgacaag acctgttagc cagtctgtgc aacctaactc tgaagattta tctgttaagc
     2461 ataaagactg gcagcaacta gtgcataaat accaaggaat taaggcggca agacagtctt
     2521 tagagggtgg ggtgctatac gcttggcttt accgacatga cagggattgg ctagttcact
     2581 ggaatcaaca gcatcaacaa gagcgtctgg cacccgcccc tagagttgat tggaaccaaa
     2641 gagatcgaat tgctgtacga caactattaa gaatcataaa gcgtctagat agtagccttg
     2701 atcacccaag agcgacatcg agctggctgt taaagcaaac tcctaacgga acctctcttg
     2761 caaaaaatct acagaaactg cctttggtag cgctttgctt aaagcgttac tcagagagtg
     2821 tggaagatta tcaaattaga cggattagcc aagcttttat taagcttaaa caggaagatg
     2881 ttgagcttag gcgctggcga ttattaagaa gtgcaacgtt atctaaagag cggataactg
     2941 aggaagcaca aagattcttg gaaatggttt atggggaaga gtgagtggtt aggctagcta
     3001 catttaatga caatgtgcag gttgtacata ttggtcattt attccgtaac tcgggtcata
     3061 aggagtggcg tatttttgtt tggtttaatc caatgcaaga acggaaatgg actcgattta
     3121 ctcatttgcc tttattaagt cgagctaagg tggttaacag tacaacaaag caaataaata
     3181 aggcggatcg tgtgattgag tttgaagcat cggatcttca acgagccaaa ataatcgatt
     3241 ttcctaatct ctcgtccttt gcttccgtac gcaacaagga tggagcgcag agttcattta
     3301 tttacgaagc tgaaacacca tatagcaaga ctcgttatca catcccacag ttagagctag
     3361 ctcggtcatt atttttaggg gatcctctag agtcgacctg caggcatgca agcttggctg
     3421 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
     3481 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
     3541 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt
     3601 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     3661 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
     3721 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
     3781 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
     3841 ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3901 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3961 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4021 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4081 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4141 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     4201 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4261 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4321 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4381 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4441 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     4501 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4561 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4621 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4681 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4741 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4801 gaccaagttt actcatatat actttagatt gatttacgcg ccctgtagcg gcgcattaag
     4861 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4921 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccgccggcc agcctcgcag
     4981 agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc
     5041 gctcgcgggt gggcctactt cacctatcct gcccggcggc atcaccggcg ccacaggtgc
     5101 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     5161 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     5221 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     5281 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgat ccccgacagt
     5341 aagacgggta agcctgttga tgataccgct gccttactgg gtgcattagc cagtctgaat
     5401 gacctgtcac gggataatcc gaagtggtca gactggaaaa tcagagggca ggaactgctg
     5461 aacagcaaaa agtcagatag caccacatag cagacccgcc ataaaacgcc ctgagaagcc
     5521 cgtgacgggc ttttcttgta ttatgggtag tttccttgca tgaatccata aaaggcgcct
     5581 gtagtgccat ttacccccat tcactgccag agccgtgagc gcagcgaact gaatgtcacg
     5641 aaaaagacag cgactcaggt gcctgatggt cggagacaaa aggaatattc agcgatttgc
     5701 ccgagcttgc gagggtgcta cttaagcctt tagggtttta aggtctgttt tgtagaggag
     5761 caaacagcgt ttgcgacatc cttttgtaat actgcggaac tgactaaagt agtgagttat
     5821 acacagggct gggatctatt ctttttatct ttttttattc tttctttatt ctataaatta
     5881 taaccacttg aatataaaca aaaaaaacac acaaaggtct agcggaattt acagagggtc
     5941 tagcagaatt tacaagtttt ccagcaaagg tctagcagaa tttacagata cccacaactc
     6001 aaaggaaaag gactagtaat tatcattgac tagcccatct caattggtat agtgattaaa
     6061 atcacctaga ccaattgaga tgtatgtctg aattagttgt tttcaaagca aatgaactag
     6121 cgattagtcg ctatgactta acggagcatg aaaccaagct aattttatgc tgtgtggcac
     6181 tactcaaccc cacgattgaa aaccctacaa ggaaagaacg gacggtatcg ttcacttata
     6241 accaatacgt tcagatgatg aacatcagta gggaaaatgc ttatggtgta ttagctaaag
     6301 caaccagaga gctgatgacg agaactgtgg aaatcaggaa tcctttggtt aaaggctttg
     6361 agattttcca gtggacaaac tatgccaagt tctcaagcga aaaattagaa ttagttttta
     6421 gtgaagagat attgccttat cttttccagt taaaaaaatt cataaaatat aatctggaac
     6481 atgttaagtc ttttgaaaac aaatactcta tgaggattta tgagtggtta ttaaaagaac
     6541 taacacaaaa gaaaactcac aaggcaaata tagagattag ccttgatgaa tttaagttca
     6601 tgttaatgct tgaaaataac taccatgagt ttaaaaggct taaccaatgg gttttgaaac
     6661 caataagtaa agatttaaac acttacagca atatgaaatt ggtggttgat aagcgaggcc
     6721 gcccgactga tacgttgatt ttccaagttg aactagatag acaaatggat ctcgtaaccg
     6781 aacttgagaa caaccagata aaaatgaatg gtgacaaaat accaacaacc attacatcag
     6841 attcctacct acataacgga ctaagaaaaa cactacacga tgctttaact gcaaaaattc
     6901 agctcaccag ttttgaggca aaatttttga gtgacatgca aagtaagtat gatctcaatg
     6961 gttcgttctc atggctcacg caaaaacaac gaaccacact agagaacata ctggctaaat
     7021 acggaaggat ctgaggttct tatggctctt gtatctatca gtgaagcatc aagactaaca
     7081 aacaaaagta gaacaactgt tcaccgttac atatcaaagg gaaaactgtc catatgcaca
     7141 gatgaaaacg gtgtaaaaaa gatagataca tcagagcttt tacgagtttt tggtgcattt
     7201 aaagctgttc accatgaaca gatcgacaat gtaacagatg aacagcatgt aacacctaat
     7261 agaacaggtg aaaccagtaa aacaaagcaa ctagaacatg aaattgaaca cctgagacaa
     7321 cttgttacag ctcaacagtc acacatagac agcctgaaac aggcgatgct gcttatcgaa
     7381 tcaaagctgc cgacaacacg ggagccagtg acgcctcccg tggggaaaaa atcatggcaa
     7441 ttctggaaga aatagcgctt tcagcctgtg ggcggacaaa atagttggga actgggaggg
     7501 gtggaaatgg agtttttaag gattatttag ggaagagtga caaaatagat gggaactggg
     7561 tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc
     7621 acttatctgg ttggtcgaca ctagtattac cctgttatcc ctagatttaa atgatatcgg
     7681 atcctagtaa gccacgtttt aatcgttagt tagttatctt caagagaaaa tgtaatcaca
     7741 ctggctcacc ttcgggtggg cctttctgcg tttataagga gacactttga attcgagctc
     7801 aggcgcgcca gcggccgcac attcactgaa ctttgacgta ccggcacatg tcgtctaaac
     7861 cataagttga catatctaaa gcataagttg acatgatctt gcatcataag ctgacatagc
     7921 ctaaacctgt agtaaagtaa caacaggttg gtggtgtgtt tttaaatgat atcggatcct
     7981 agtaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     8041 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     8101 tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt
     8161 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt
     8221 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     8281 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     8341 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     8401 gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     8461 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     8521 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     8581 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     8641 taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     8701 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     8761 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     8821 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     8881 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     8941 gagcattacg ctgacttgac gggacggcgg ctttgttgaa taaatgatgc agttaagtgc
     9001 ctgctgtgta tctcgaacag attgctgaag tggtaaaata acaacaccgc aggggcaggg
     9061 cgtaggggtt gatgtcactc tatgtcagct tatgggtaat ttttatgtca acttatgggt
     9121 tataaaacac tcgtaagtca ctgatttatg gttgtgcgtt atgtcaactt atggtttaga
     9181 cgacagcaca acctcaccca gtccttcttc ggggggcggc cgcgtttaaa cgctgaggga
     9241 aagttcgaat ccgacgtccc tcctgtgtga aattgttatg gcgactcgag ttcgaatgtc
     9301 tcctcagccc tgcaggaagc tttacgacca gtctaaaaag cgcctgaatt cgcgaccttc
     9361 tcgttactga caggaaaatg ggccattggc aaccagggaa agatgaacgt gatgatgttc
     9421 acaatttgct gaattgtggt gatgtgatgc tcaccgcatt tccttaatta acgccggatg
     9481 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     9541 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     9601 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     9661 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg
     9721 gatggccttt ttgcgtttag ttagttatct cctaggacgc gccacggccg cgtcgacccc
     9781 acgcccctct ttaatacgac gggcaatttg cacttcagaa aatgaagagt ttgctttagc
     9841 cataacaaaa gtccagtatg ctttttcaca gcataactgg actgatttca gtttacaact
     9901 attctgtcta gtttaagact ttattgtcat agtttagatc tattttgttc agtttaagac
     9961 tttattgtcc gcccacaggc cacgatgcgt ccggcgtaga ggatctgctc atgtttgaca
    10021 gcttatcatc gatgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct
    10081 atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc
    10141 tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca
    10201 ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt
    10261 ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc
    10321 gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg
    10381 cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc
    10441 gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt
    10501 aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga
    10561 atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg
    10621 ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag
    10681 ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc
    10741 ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg
    10801 tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt
    10861 gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc
    10921 ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg
    10981 atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg
    11041 tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccaa
    11101 accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa
    11161 aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg
    11221 cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg
    11281 ctttttatcg caactctcta ctgtttctcc atacccgttt ttttgggcta gcgaattcga
    11341 gctcggtacc caagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat
    11401 gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag
    11461 tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa gactatattc
    11521 catggctaac agtacaagaa gttccttctt caggtcgttc ccaccgtatt tattctcata
    11581 agacgggacg agtccatcat ttgctatctg acttagagct tgctgttttt ctcagtcttg
    11641 agtgggagag cagcgtgcta gatatacgcg agcagttccc cttattacct agtgatacca
    11701 ggcagattgc aatagatagt ggtattaagc atcctgttat tcgtggtgta gatcaggtta
    11761 tgtctactga ttttttagtg gactgcaaag atggtccttt tgagcagttt gctattcaag
    11821 tcaaacctgc agcagcctta caagacgagc gtaccttaga aaaactagaa ctagagcgtc
    11881 gctattggca gcaaaagcaa attccttggt tcatttttac tgataaagaa ataaatcccg
    11941 tagtaaaaga aaatattgaa tggctttatt cagtgaaaac agaagaagtt tctgcggagc
    12001 ttttagcaca actatcccca ttggcccata tcctgcaaga aaaaggagat gaaaacatta
    12061 tcaatgtctg taagcaggtt gatattgctt atgatttgga gttaggcaaa acattgagtg
    12121 agatacgagc cttaaccgca aatggtttta ttaagttcaa tatttataag tctttcaggg
    12181 caaataagtg tgcagatctc tgtattagcc aagtagtgaa tatggaggag ttgcgctatg
    12241 tggcaaatta atgaggttgt gctatttgat aatgatccgt atcgcatttt ggctatagag
    12301 gatggccaag ttgtctggat gcaaataagc gctgataaag gagttccaca agctagggct
    12361 gagttgttgc taatgcagta tttagatgaa ggccgcttag ttagaactga tgacccttat
    12421 gtacatcttg atttagaaga gccgtctgta gattctgtca gcttccagaa gcgcgaggag
    12481 gattatcgaa aaattcttcc tattattaat agtaaggatc gtttcgaccc taaagtcaga
    12541 agcgaactcg ttgagcatgt ggtccaagaa cataaggtta ctaaggctac agtttataag
    12601 ttgttacgcc gttactggca gcgtggtcaa acgcctaatg cattaattcc tgactacaaa
    12661 aacagcggtg caccagggga aagacgttca gcgacaggaa cagcaaagat tggccgagcc
    12721 agagaatatg gtaagggtga aggaaccaag gtaacgcccg agattgaacg cctttttagg
    12781 ttgaccatag aaaagcacct gttaaatcaa aaaggtacaa agaccaccgt tgcctataga
    12841 cgatttgtgg acttgtttgc tcagtatttt cctcgcattc cccaagagga ttacccaaca
    12901 ctacgtcagt ttcgttattt ttatgatcga gaatacccta aagctcagcg cttaaagtct
    12961 agagttaaag caggggtata taaaaaagac gtacgaccct taagtagtac agccacttct
    13021 caggcgttag gccctgggag tcgttatgag attgatgcca cgattgctga tatttattta
    13081 gtggatcatc atgatcgcca aaaaatcata ggaagaccaa cgctttacat tgtgattgat
    13141 gtgtttagtc ggatgatcac gggcttttat atcggctttg aaaatccgtc ttatgtggtg
    13201 gcgatgcagg cttttgtaaa tgcttgctct gacaaaacgg ccatttgtgc ccagcatgat
    13261 attgagatta gtagctcaga ctggccgtgt gtaggtttgc cagatgtgtt gctagcggac
    13321 cgtggcgaat taatgagtca tcaggtcgaa gccttagttt ctagttttaa tgtgcgagtg
    13381 gaaagtgctc cacctagacg tggcgatgct aaaggcatag tggaaagcac ttttagaaca
    13441 ctacaagccg agtttaagtc ctttgcacct ggcattgtag agggcagtcg gatcaaaagc
    13501 catggtgaaa cagactatag gttagatgca tctctgtcgg tatttgagtt cacacaaatt
    13561 attttgcgta cgatcttatt cagaaataac catctggtga tggataaata cgatcgagat
    13621 gctgattttc ctacagattt accgtctatt cctgtccagc tatggcaatg gggtatgcag
    13681 catcgtacag gtagtttaag ggctgtggag caagagcagt tgcgagtagc gttactgcct
    13741 cgccgaaagg tctctatttc ttcatttggc gttaatttgt ggggtttgta ttactcgggg
    13801 tcagagattc tgcgtgaggg ttggttgcag cggagcactg atatagctag acctcaacat
    13861 ttagaagcgg cttatgaccc agtgctggtt gatacgattt atttgtttcc gcaagttggc
    13921 agccgtgtat tttggcgctg taatctgacg gaacgtagtc ggcagtttaa aggtctctca
    13981 ttttgggagg tttgggatat acaagcacaa gaaaaacaca ataaagccaa tgcgaagcag
    14041 gatgagttaa ctaaacgcag ggagcttgag gcgtttattc agcaaaccat tcagaaagcg
    14101 aataagttaa cgcccagtac tactgagccc aaatcaacac gcattaagca gattaaaact
    14161 aataaaaaag aagccgtgac ctcggagcgt aaaaaacgtg cggagcattt gaagccaagc
    14221 tcttcaggtg atgaggctaa agttattcct ttcaacgcag tggaagcgga tgatcaagaa
    14281 gattacagcc tacccacata cgtgcctgaa ttatttcagg atccaccaga aaaggatgag
    14341 tcatgagtgc tacccggatt caagcagttt atcgtgatac gggggtagag gcttatcgtg
    14401 ataatccttt tatcgaggcc ttaccaccat tacaagagtc agtgaatagt gctgcatcac
    14461 tgaaatcctc tttacagctt acttcctctg acttgcaaaa gtcccgtgtt atcagagctc
    14521 ataccatttg tcgtattcca gatgactatt ttcag
//
